Plant Extracellular Nanovesicle-Loaded Hydrogel for Topical Antibacterial Wound Healing In Vivo DOI

Saroj Saroj,

Sunita Saha, Akbar Ali

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Bacterial infections impede wound healing and pose significant challenges in clinical care. There is an immediate need for safe targeted antivirulence agents to fight bacterial effectively. In this regard, bioderived nanovesicles have shown promise. This work demonstrated antibacterial properties of extracellular derived from plant (mint) leaf juice (MENV). A hydrogel (HG) was developed using oxidized alginate chitosan loaded with MENVs (MENV-HG). formulation investigated topical HG dressings treat Gram-positive Micrococcus luteus Gram-negative Escherichia coli-invasive wounds. The injectable, biocompatible (>95% cell viable), nonhemolytic (<5% hemolytic capacity), self-healing exhibited strong physical mechanical interactions the bacteria cells (MENV-HG-treated were significantly more elastic compared control both M. (1.01 ± 0.3 MPa, p < 0.005 vs 5.03 2.6) E. coli (5.81 2.1 MPa 10.81 3.8, 0.005). MENV-HG topically applied on wounds a slow MENV release profile, ensuring effective healing. These vivo results decreased inflammation expedited within 10 days treatment (wound area closure 99% 87% control). Taken together, MENV-HGs potential scalable sustainable dressing strategy that works satisfactorily bacteria-infected be validated trials.

Language: Английский

Chitosan-based hydrogels in cancer therapy: Drug and gene delivery, stimuli-responsive carriers, phototherapy and immunotherapy DOI

Hailin Zhu,

Haowei Sun, Jingyuan Dai

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 282, P. 137047 - 137047

Published: Nov. 1, 2024

Language: Английский

Citations

5
Chitosan-based nanoarchitectures for siRNA delivery in cancer therapy: A review of pre-clinical and clinical importance DOI

Xiaobo Bian,

Xiaopeng Yu, Shiyang Lu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137708 - 137708

Published: Nov. 1, 2024

Language: Английский

Citations

5
Application of graphene oxide in Agrobacterium-mediated genetic transformation and construction of a novel DNA delivery system for watermelon DOI Creative Commons
Yuanyuan Qin, Shumei Hua, Lili Zhu

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 14, 2025

Graphene oxide (GO) is widely used in biotechnology. The purpose of this study was to improve the efficiency genetic transformation by constructing a delivery system based on GO. First, GO applied traditional scheme for watermelons. We hydroponics and tissue culture methods determine optimal concentration watermelon plant growth, we then found that can inhibit growth Agrobacterium tumefaciens promote explants. This discovery simplify replacement various media after explant infection, regeneration rate transgenic plants, reduce experimental costs. To transformation, polymer-functionalized graphene nanoparticle (GO-PEG-PEI) nanodelivery constructed, results showed GO-PEG-PEI transfer pCAMBIA1300-GFP plasmids into intact cells. sheet-like effectively load GFP form small GO-PEG-PEI-GFP complexes, which deliver research provides new technique molecular breeding.

Language: Английский

Citations

0
Plant‐Based Anticancer Compounds With a Focus on Breast Cancer DOI Creative Commons
Amin Moradi Hasan‐Abad, Amir Atapour, Ali Sobhani‐Nasab

et al.

Cancer Reports, Journal Year: 2024, Volume and Issue: 7(10)

Published: Oct. 1, 2024

Breast cancer is a common form of among women characterized by the growth malignant cells in breast tissue. The most treatments for this condition include chemotherapy, surgical intervention, radiation therapy, hormone and biological therapy. primary issues associated with chemotherapy therapy are their adverse events significant financial burden patients underdeveloped countries. This highlights need to explore develop superior therapeutic options that less detrimental more economically efficient. Plants provide an abundant supply innovative compounds present promising new avenue investigating cancer. derivations undergoing revolution due reduced toxicity, expediency, cost-effectiveness, safety, simplicity comparison conventional treatment methods. Natural products considered candidates development anticancer drugs, perhaps diverse pleiotropic effects on target events. plant-derived limited while leaving healthy unaffected. Identification strong properties plant-based medications might be crucial steps Although bioactive have potent properties, they also drawbacks resolved before application clinical trials improved approved drugs. study aims give comprehensive information known compounds, including sources molecular mechanisms actions, along opportunities challenges therapies.

Language: Английский

Citations

1
Graphene/carbohydrate polymer composites as emerging hybrid materials in tumor therapy and diagnosis DOI
Zhenwang Zhang, Jinxiang Wang, Lingmi Hou

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 287, P. 138621 - 138621

Published: Dec. 10, 2024

Language: Английский

Citations

1
Plant Extracellular Nanovesicle-Loaded Hydrogel for Topical Antibacterial Wound Healing In Vivo DOI

Saroj Saroj,

Sunita Saha, Akbar Ali

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Bacterial infections impede wound healing and pose significant challenges in clinical care. There is an immediate need for safe targeted antivirulence agents to fight bacterial effectively. In this regard, bioderived nanovesicles have shown promise. This work demonstrated antibacterial properties of extracellular derived from plant (mint) leaf juice (MENV). A hydrogel (HG) was developed using oxidized alginate chitosan loaded with MENVs (MENV-HG). formulation investigated topical HG dressings treat Gram-positive Micrococcus luteus Gram-negative Escherichia coli-invasive wounds. The injectable, biocompatible (>95% cell viable), nonhemolytic (<5% hemolytic capacity), self-healing exhibited strong physical mechanical interactions the bacteria cells (MENV-HG-treated were significantly more elastic compared control both M. (1.01 ± 0.3 MPa, p < 0.005 vs 5.03 2.6) E. coli (5.81 2.1 MPa 10.81 3.8, 0.005). MENV-HG topically applied on wounds a slow MENV release profile, ensuring effective healing. These vivo results decreased inflammation expedited within 10 days treatment (wound area closure 99% 87% control). Taken together, MENV-HGs potential scalable sustainable dressing strategy that works satisfactorily bacteria-infected be validated trials.

Language: Английский

Citations

0