Chemistry - An Asian Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 22, 2025
Abstract
Cathepsin
S
(CatS)
is
a
lysosomal
enzyme
belonging
to
the
papain
family
of
cysteine
proteases.
Studies
have
demonstrated
that
CatS
plays
pivotal
role
in
antigen
presentation
and
associated
with
Alzheimer's
disease,
atherosclerosis,
obesity,
positioning
it
as
potential
target
related
disorders.
Consequently,
development
selective
straightforward
method
for
detecting
crucial.
In
this
study,
we
introduce
colorimetric
approach
utilizing
gold
nanoparticles
(AuNPs)
CatS‐specific
peptides
detection
activity.
The
cleavage
peptides,
PMGAPC
or
PMGAPC‐NH
2
,
can
stabilize
AuNPs
hydrosaline
solution
without
aggregation.
Upon
hydrolysis
by
CatS,
aggregate
color
change
from
red
blue.
After
optimizing
size
concentration
limits
were
both
be
10
nM,
when
used
protect
AuNPs.
Therefore,
assay,
which
employs
AuNPs,
enables
not
only
but
also
simple
visible
naked
eye.
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(5), P. 275 - 275
Published: April 29, 2025
Ecosystem
pollution
by
mercury
ions
(Hg2+)
is
a
major
health
concern,
yet
classical
analytical
methods
for
analysis
are
limited.
This
paper
reviews
the
advances
in
Hg2+
detection
using
DNA
as
recognition
elements
sensors.
molecule
inexpensive,
simple,
and
appropriate
real-time
of
Hg2+.
discusses
DNA-based
sensors
that
were
used
These
can
be
carried
out
electrochemistry,
field
effect
transistors
(FET),
Raman
spectroscopy,
colorimetry,
fluorescence
resonance
energy
transfer
(FRET).
The
principles
advantages
these
also
revealed.
Finally,
provides
an
overview
prospects
potential
challenges
field.
Chemistry - An Asian Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 22, 2025
Abstract
Cathepsin
S
(CatS)
is
a
lysosomal
enzyme
belonging
to
the
papain
family
of
cysteine
proteases.
Studies
have
demonstrated
that
CatS
plays
pivotal
role
in
antigen
presentation
and
associated
with
Alzheimer's
disease,
atherosclerosis,
obesity,
positioning
it
as
potential
target
related
disorders.
Consequently,
development
selective
straightforward
method
for
detecting
crucial.
In
this
study,
we
introduce
colorimetric
approach
utilizing
gold
nanoparticles
(AuNPs)
CatS‐specific
peptides
detection
activity.
The
cleavage
peptides,
PMGAPC
or
PMGAPC‐NH
2
,
can
stabilize
AuNPs
hydrosaline
solution
without
aggregation.
Upon
hydrolysis
by
CatS,
aggregate
color
change
from
red
blue.
After
optimizing
size
concentration
limits
were
both
be
10
nM,
when
used
protect
AuNPs.
Therefore,
assay,
which
employs
AuNPs,
enables
not
only
but
also
simple
visible
naked
eye.
