Facile Universal Strategy of Presenting Multifunctional Short Peptides for Customizing Desired Surfaces DOI Creative Commons

Ruofei Lu,

Bingyang Zhao,

Kaiyuan Huo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 19, 2024

Abstract Interfacial properties determine biomaterial performances, such as cell adhesion, signal exchange, and biomineralization, which affect the tissue repair cycle efficiency of clinical applications. Peptides, short protein sequences that have defined functionalities, are highly stable easy to synthesize enormous potential reshape interfacial properties. However, lack a universal strategy for presenting peptides on various substrates substantially hinders application peptides. In this study, we report facile customizing desired functionalities by well-known layer-by-layer (LbL) technique through assembly polyphenols with positively charged peptide-coupling functional sequences. Polyphenol–peptide interactions were elucidated in detail assembling possessing different characteristics (charged, uncharged, hydrophobic, sequence length) combination molecular dynamics simulations, isothermal titration calorimetry further revealed favorable enthalpy change due electrostatic is main driving force polyphenols. LbL coatings assembled from exhibited good substrate generalization, stability, proliferation, antioxidant properties, when prepared hollow capsules sacrificing template, significant pH ultrasound stimulation responses, could be suitable candidates drug carriers. Most importantly, utilized present molecules (such arginyl–glycyl–aspartic acid (RGD), adhesion motif; CM15, an antibacterial peptide; PEG, antifouling surface) surfaces. This study not only provides new insights into understanding regulation between proteins/peptides but also paves way toward functionalization biomaterials.

Language: Английский

Protein-based bioactive coatings: from nanoarchitectonics to applications DOI
Chengyu Fu,

Zhengge Wang,

Xingyu Zhou

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(3), P. 1514 - 1551

Published: Jan. 1, 2024

Assembly strategy and application direction of protein-based bioactive coatings.

Language: Английский

Citations

24
Facile universal strategy of presenting multifunctional short peptides for customizing desired surfaces DOI Creative Commons

Ruofei Lu,

Bingyang Zhao,

Kaiyuan Huo

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 2, 2025

Interfacial properties determine biomaterial performances, such as cell adhesion, signal exchange, and biomineralization, which affect the tissue repair cycle efficiency of clinical applications. Peptides, short protein sequences that have defined functionalities, are highly stable easy to synthesize enormous potential reshape interfacial properties. However, lack a universal strategy for presenting peptides on various substrates substantially hinders application peptides. In this study, we report facile customizing desired functionalities by well-known layer-by-layer (LbL) technique through assembly polyphenols with positively charged peptide-coupling functional sequences. Polyphenol–peptide interactions were elucidated in detail assembling possessing different characteristics (charged, uncharged, hydrophobic, sequence length) combination molecular dynamics simulations, isothermal titration calorimetry further revealed favorable enthalpy change due electrostatic is main driving force polyphenols. LbL coatings assembled from exhibited good substrate generalization, stability, proliferation, antioxidant properties, when prepared hollow capsules sacrificing template, significant pH ultrasound stimulation responses, could be suitable candidates drug carriers. Most importantly, utilized present molecules (such arginyl–glycyl–aspartic acid (RGD), adhesion motif; CM15, an antibacterial peptide; PEG, antifouling surface) surfaces. This study not only provides new insights into understanding regulation between proteins/peptides but also paves way toward functionalization biomaterials.

Language: Английский

Citations

1
A strategy of de novo peptides for customizing supramolecular self-assembly coating with desired biological functionalities DOI
Na Li, Zeyu Shou,

Wenjie Liu

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 154058 - 154058

Published: July 18, 2024

Language: Английский

Citations

8
Recent Progress in Protein-Polyphenol Assemblies for Biomedical Applications DOI
Yan Guo,

Xinyue Zhai,

Na Li

et al.

Langmuir, Journal Year: 2024, Volume and Issue: 40(4), P. 2005 - 2014

Published: Jan. 16, 2024

Nowadays, natural materials as smart building blocks for assembling functional have aroused extensive interest in the scientific community. Proteins and polyphenols are typical that widely used. On one hand, proteins of most versatile classes biomolecules, serving catalysts, signaling molecules, transporters, receptors, scaffolds maintain integrity cell tissue, more. other facile adhesion naturally abundant with substances their potential biomedical applications been highly attractive biomaterials fabrication. Additionally, there a variety interactions between polyphenols, mainly hydrogen bonding, hydrophobic, ionic interactions. These reversible dynamic enable to form stable protein-polyphenol assemblies inherent structures biological activities assemblies. Therefore, can be applied design advanced applications. Herein, recent progress particles, capsules, coatings, hydrogels is summarized, preparation application these introduced detail, future field prospected.

Language: Английский

Citations

7
Presenting dual-functional peptides on implant surface to direct in vitro osteogenesis and in vivo osteointegration DOI Creative Commons

Nan Hui,

Yong Gou,

Chunkai Bao

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 27, P. 101108 - 101108

Published: June 4, 2024

The complex biological process of osseointegration and the bio-inertness bone implants are major reasons for high failure rate long-term implants, have also promoted rapid development multifunctional implant coatings in recent years. Herein, through special design peptides, we use layer-by-layer assembly technology to simultaneously display two peptides with different functions on surface address this issue. A variety characterization techniques (ellipsometry, atomic force microscopy, photoelectron spectroscopy, dissipation-quartz crystal microbalance) were used study detail preparation dual peptide functional coating physical chemical properties, such as composition, mechanical modulus, stability, roughness coating. Compared single coatings, dual-peptide functionalized had much better performances antioxidant, cellular adhesion early stage, proliferation osteogenic differentiation long term, well vivo osteogenesis capabilities. These findings will promote designs a coping strategy complexity during osteointegration.

Language: Английский

Citations

5
Engineering tunable dual peptide hybrid coatings promote osseointegration of implants DOI Creative Commons
Zeyu Shou, Zhibiao Bai, Han Zhou

et al.

Materials Today Bio, Journal Year: 2023, Volume and Issue: 24, P. 100921 - 100921

Published: Dec. 16, 2023

Utilizing complementary bioactive peptides is a promising surface engineering strategy for bone regeneration on osteogenesis. In this study, we designed block peptides, (Lysine)

Language: Английский

Citations

13
Construction of Mg2+ loaded multifunctional casein phosphopeptide/alendronate sodium antioxidative coating for repairing osteoporotic fracture DOI

Shuoshuo Yang,

Yuhua Huang, Yonggang Lv

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 305, P. 141333 - 141333

Published: Feb. 19, 2025

Language: Английский

Citations

0
The De Novo Strategy for Bifunctional Peptides Coating to Enhance Osteointegration Capacity of the Implant DOI
Siwang Hu, Zeyu Shou, Chengwei Xu

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: unknown, P. 114642 - 114642

Published: March 1, 2025

Language: Английский

Citations

0
Induction of multicomponent peptide assembly via solvent exchange strategy for enhanced structure and bioactivities DOI
Yajuan Li,

David Julian McClements,

Fei Pan

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 500, P. 157484 - 157484

Published: Nov. 1, 2024

Language: Английский

Citations

3
Impact of pH on sodium caseinate binding and structural changes on montmorillonite surface DOI
Mohamed Brahmi, Kamal Essifi, Abdesselam Tahani

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 290, P. 138978 - 138978

Published: Dec. 19, 2024

Language: Английский

Citations

1