Cell Sheets Formation Enhances Therapeutic Effects of Human Umbilical Cord Mesenchymal Stem Cells on Spinal Cord Injury DOI Creative Commons
Yulin Zhao, Zhixian Wu, Yuchen Zhou

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2024, Volume and Issue: 30(12)

Published: Dec. 1, 2024

ABSTRACT Background In recent years, the utilization of stem cell therapy and sheet technology has emerged as a promising approach for addressing spinal cord injury (SCI). However, most appropriate type mechanism action remain unclear at this time. This study sought to develop an SCI rat model evaluate therapeutic effects human umbilical mesenchymal (hUC‐MSC) sheets in model. Furthermore, mechanisms underlying vascular repair effect hUC‐MSC following were investigated. Methods A temperature‐responsive culture method was employed preparation sheets. The extracellular matrix (ECM) produced by hUC‐MSCs serves two distinct yet interrelated purposes. Firstly, it acts biologically active scaffold transplanted cells, facilitating their attachment proliferation. Secondly, provides mechanical support bridges stumps, thereby restoration function. formation cavity within evaluated using Hematoxylin Eosin (H&E) staining method. Subsequently, endothelial cells cultivated with conditioned medium (CM) obtained from or pro‐angiogenic impact (MSC‐CM) (CS‐CM) through CCK‐8 assay, wound healing tube assay vitro context. development glial scars, blood vessels, neurons, axons assessed immunofluorescence staining. Results comparison hUC‐MSCs, demonstrated more pronounced capacity facilitate induce regeneration newborn neurons site, while also reducing scar significantly enhancing motor function rats. Notably, under identical conditions, been associated paracrine increase ability themselves secrete growth factors. During course experiment, observed that secretion uPAR among factors present MSC‐CM CS‐CM. finding subsequently corroborated subsequent experiments, wherein promote angiogenesis via PI3K/Akt signaling pathway. Conclusion creation not only enhances biological but effectively retains locally injury. Therefore, transplantation can maximize greatly formation, promoting axons. Additionally, research findings prove activate pathway enhance angiogenesis. transfer entire sheets, absence introduction additional exogenous synthetic biomaterials, further augment potential clinical application.

Language: Английский

Cell Sheets Formation Enhances Therapeutic Effects of Human Umbilical Cord Mesenchymal Stem Cells on Spinal Cord Injury DOI Creative Commons
Yulin Zhao, Zhixian Wu, Yuchen Zhou

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2024, Volume and Issue: 30(12)

Published: Dec. 1, 2024

ABSTRACT Background In recent years, the utilization of stem cell therapy and sheet technology has emerged as a promising approach for addressing spinal cord injury (SCI). However, most appropriate type mechanism action remain unclear at this time. This study sought to develop an SCI rat model evaluate therapeutic effects human umbilical mesenchymal (hUC‐MSC) sheets in model. Furthermore, mechanisms underlying vascular repair effect hUC‐MSC following were investigated. Methods A temperature‐responsive culture method was employed preparation sheets. The extracellular matrix (ECM) produced by hUC‐MSCs serves two distinct yet interrelated purposes. Firstly, it acts biologically active scaffold transplanted cells, facilitating their attachment proliferation. Secondly, provides mechanical support bridges stumps, thereby restoration function. formation cavity within evaluated using Hematoxylin Eosin (H&E) staining method. Subsequently, endothelial cells cultivated with conditioned medium (CM) obtained from or pro‐angiogenic impact (MSC‐CM) (CS‐CM) through CCK‐8 assay, wound healing tube assay vitro context. development glial scars, blood vessels, neurons, axons assessed immunofluorescence staining. Results comparison hUC‐MSCs, demonstrated more pronounced capacity facilitate induce regeneration newborn neurons site, while also reducing scar significantly enhancing motor function rats. Notably, under identical conditions, been associated paracrine increase ability themselves secrete growth factors. During course experiment, observed that secretion uPAR among factors present MSC‐CM CS‐CM. finding subsequently corroborated subsequent experiments, wherein promote angiogenesis via PI3K/Akt signaling pathway. Conclusion creation not only enhances biological but effectively retains locally injury. Therefore, transplantation can maximize greatly formation, promoting axons. Additionally, research findings prove activate pathway enhance angiogenesis. transfer entire sheets, absence introduction additional exogenous synthetic biomaterials, further augment potential clinical application.

Language: Английский

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