A Novel Large-Particle Slow-Release Fertilizer Improves Nutrient Use Efficiency and Yield of Cassava by Boundary Layer Limitation

Agronomy, Journal Year: 2025, Volume and Issue: 15(2), P. 261 - 261

Published: Jan. 21, 2025

Cassava is a crucial food and economic crop in tropical regions globally. In response to challenges fertilizer use efficiency for cassava cultivation, which traditionally compromised by extensive leaching broad root zone distribution, novel large-particle slow-release (LPF) was developed this study. This synthesized through solution polymerization using non-metallic minerals seaweed extract. Compared conventional SFs that release 99% of nutrients within 1 min, the LPF prolonged duration 51 min under optimal synthesis conditions: drying temperature 80 °C, total extrusion force 40 t, air pressure −0.40 bar, auxiliary mineral proportion 50%, water content 15%. Microbeam characterization (e.g., FTIR) kinetic modeling revealed superior performance resulted from crystal enrichment outer layer granules, facilitating intra-particle diffusion processes imposing boundary limitations on nutrient N, P, K). Field experiments validated fertilizer. Notably, soil treated with exhibited retention topsoil (0–20 cm) both horizontally vertically. Even two-thirds relative fertilizers, also displayed significant improvements yield, partial factor productivity, agronomic 33.56%, 200.01%, 513.84%, respectively. These results indicate presents promising sustainable cultivation.

Language: Английский

Engineering of Microbial Cell Factories for Enhanced Acetic Acid and Ethanol Production Via Heterologous Overexpression of the CODH Gene in CO₂ Fermentation

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

This study evaluates the performance of carbon monoxide dehydrogenase (codh)-embedded strains in bench-scale microbial electrochemical systems (MES) for CO2 reduction to biofuels and biochemicals. fermentation efficiency was evaluated by comparing wild-type Clostridium acetobutylicum (Wild), a negative control E. coli strain lacking codh gene (NC-BL21), engineered (Eng) alone with IPTG induction (Eng+IPTG). Four were used, viz. Wild+E, NC-BL21+E, Eng+E, Eng+IPTG+E, poised potential -0.6 V applied working electrode. bicarbonate supplemented total inorganic (IC) concentration 40 g/L, retention time 60 h. The demonstrated enhanced metabolic compared strains, yielding maximum formic acid (2.1 g/L) acetic (9.3 under Eng+IPTG+E condition. Solventogenesis also influenced positively same system ethanol yield 3.9 substantially exceeding biochemicals observed (2.4 acid). exhibited superior cumulative yields (0.40 g/g), CODH-mediated charge flux stability (60 vs 5 wild type), upregulated expression key genes Wood-Ljungdahl pathway (WLP). Bioelectrochemical analysis elevated reductive catalytic currents, reduction, optimal transfer kinetics. highlights synergistic genetic engineering, specifically CODH overexpression, combined electro-fermentation biofuel biochemical production from C1 gases.

Language: Английский

Nano-micro materials regulated biocatalytic metabolism for efficient environmental remediation: fine engineering the mass and electron transfer in multicellular environments

Water Research, Journal Year: 2024, Volume and Issue: 268, P. 122759 - 122759

Published: Nov. 6, 2024

Language: Английский