Oligo‐Adenine Derived Secondary Nucleic Acid Frameworks: From Structural Characteristics to Applications DOI Creative Commons
Yuwei Hu, Itamar Willner

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(45)

Published: Aug. 26, 2024

Abstract Oligo‐adenine (polyA) is primarily known for its critical role in mRNA stability, translational status, and gene regulation. Beyond biological functions, extensive research has unveiled the diverse applications of polyA. In response to environmental stimuli, single polyA strands undergo distinctive structural transitions into secondary configurations, which are reversible upon introduction appropriate counter‐triggers. this review, we systematically summarize recent advances noncanonical structures derived from polyA, including A‐motif duplex, A‐cyanuric acid triplex, A‐coralyne‐A T ⋅ A‐T triplex. The characteristics mechanisms underlying these conformations under specific external stimuli addressed, followed by examples their stimuli‐responsive DNA hydrogels, supramolecular fibre assembly, molecular electronics switches, biosensing bioengineering, payloads encapsulation release, others. A detailed comparison polyA‐derived provided, highlighting features. Furthermore, integrating stimuli‐responsiveness conformational characteristics, advanced material development, such as pH‐cascaded hydrogels fibres exhibiting dynamic adapting cues, introduced. An outlook future developments also discussed. These derived, stimuli‐responsive, enrich arsenal “toolbox”, offering frameworks applications.

Language: Английский

Cascade DNA Structural Transitions Enable Stimuli-Responsive Hydrogels DOI
Maria Ho, Ashley Lim, Yujie Ke

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Cascade interactions are fundamental to enzyme catalysis and cellular activities, enabling dynamic adaptive responses environmental stimuli. DNA-based cascade systems have been widely employed mimic biological processes, such as immune DNAzyme catalysis, achieved mainly through the hybridization interaction. Herein, we present a DNA system involving single-stranded sequences, noncanonical cofactor-bridged duplexes, canonical duplexes construct dissociate hydrogel matrices. In this work, thymine-rich oligonucleotides (T-strands) exist random coils in buffer at pH 7.2. Upon introduction of low-molecular-weight cofactor, melamine (MA), supramolecular configuration, termed T-MA-T duplex, is formed. Subsequent addition adenine-rich (A-strands) leads replacement MA cofactors formation more energetically favorable A-T duplex structures. These consecutive structural transitions further utilized bridging elements stimuli-responsive hydrogels, facilitating liquid-hydrogel-liquid phase transitions. Moreover, demonstrate precisely controlled release profiles doxorubicin from hydrogel. This approach, leveraging both configurations triggered transitions, opens avenues for developing molecular switches, electronic nanodevices, materials, other advanced applications.

Language: Английский

Citations

0
Oligo‐Adenine Derived Secondary Nucleic Acid Frameworks: From Structural Characteristics to Applications DOI Creative Commons
Yuwei Hu, Itamar Willner

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(45)

Published: Aug. 26, 2024

Abstract Oligo‐adenine (polyA) is primarily known for its critical role in mRNA stability, translational status, and gene regulation. Beyond biological functions, extensive research has unveiled the diverse applications of polyA. In response to environmental stimuli, single polyA strands undergo distinctive structural transitions into secondary configurations, which are reversible upon introduction appropriate counter‐triggers. this review, we systematically summarize recent advances noncanonical structures derived from polyA, including A‐motif duplex, A‐cyanuric acid triplex, A‐coralyne‐A T ⋅ A‐T triplex. The characteristics mechanisms underlying these conformations under specific external stimuli addressed, followed by examples their stimuli‐responsive DNA hydrogels, supramolecular fibre assembly, molecular electronics switches, biosensing bioengineering, payloads encapsulation release, others. A detailed comparison polyA‐derived provided, highlighting features. Furthermore, integrating stimuli‐responsiveness conformational characteristics, advanced material development, such as pH‐cascaded hydrogels fibres exhibiting dynamic adapting cues, introduced. An outlook future developments also discussed. These derived, stimuli‐responsive, enrich arsenal “toolbox”, offering frameworks applications.

Language: Английский

Citations

3
Oligo‐Adenine Derived Secondary Nucleic Acid Frameworks: From Structural Characteristics to Applications DOI Creative Commons
Yuwei Hu, Itamar Willner

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(45)

Published: Aug. 26, 2024

Abstract Oligo‐adenine (polyA) is primarily known for its critical role in mRNA stability, translational status, and gene regulation. Beyond biological functions, extensive research has unveiled the diverse applications of polyA. In response to environmental stimuli, single polyA strands undergo distinctive structural transitions into secondary configurations, which are reversible upon introduction appropriate counter‐triggers. this review, we systematically summarize recent advances noncanonical structures derived from polyA, including A‐motif duplex, A‐cyanuric acid triplex, A‐coralyne‐A T ⋅ A‐T triplex. The characteristics mechanisms underlying these conformations under specific external stimuli addressed, followed by examples their stimuli‐responsive DNA hydrogels, supramolecular fibre assembly, molecular electronics switches, biosensing bioengineering, payloads encapsulation release, others. A detailed comparison polyA‐derived provided, highlighting features. Furthermore, integrating stimuli‐responsiveness conformational characteristics, advanced material development, such as pH‐cascaded hydrogels fibres exhibiting dynamic adapting cues, introduced. An outlook future developments also discussed. These derived, stimuli‐responsive, enrich arsenal “toolbox”, offering frameworks applications.

Language: Английский

Citations

0