Phenylboronic Acid-Modified Polyethyleneimine: A Glycan-Targeting Anti-Biofilm Polymer for Inhibiting Bacterial Adhesion to Mucin and Enhancing Antibiotic Efficacy DOI Creative Commons

Lorcan J. P. Rooney,

Andrew Marshall, Michael M. Tunney

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Bacterial biofilms present significant therapeutic challenges due to their resistance conventional antimicrobial treatment. Mucins typically serve as a protective barrier against pathogens, yet certain bacteria, such Pseudomonas aeruginosa (P. aeruginosa), can exploit these glycoproteins attachment sites for biofilm formation. This study introduces boronic acid-functionalized polyethyleneimine (PEI-BA) promising antibiofilm agent that effectively blocks bacterial adhesion mucin-rich surfaces. Through the multivalent presentation of acid groups, PEI-BA reversibly forms boronate ester bonds with mucin glycans, creating barrier. Our findings show prevents through nonbactericidal mechanism, potentially reducing risk development. Notably, synergizes antibiotic, tobramycin, significantly enhancing inhibition compared either treatment alone. Systematic evaluation formulations identified optimal functionalization levels, balancing glycan-binding capability solubility. From biomaterials design perspective, we demonstrate how rational polymer modification transform potent but cytotoxic (i.e., PEI) into safe and effective material, opening further possibilities managing biofilm-associated infections in clinical settings. work establishes acid-based nanomaterials candidates prevention antibiotic enhancement, particularly conditions like cystic fibrosis, where mucin-bacterial interactions contribute disease progression.

Language: Английский

Phenylboronic Acid-Modified Polyethyleneimine: A Glycan-Targeting Anti-Biofilm Polymer for Inhibiting Bacterial Adhesion to Mucin and Enhancing Antibiotic Efficacy DOI Creative Commons

Lorcan J. P. Rooney,

Andrew Marshall, Michael M. Tunney

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Bacterial biofilms present significant therapeutic challenges due to their resistance conventional antimicrobial treatment. Mucins typically serve as a protective barrier against pathogens, yet certain bacteria, such Pseudomonas aeruginosa (P. aeruginosa), can exploit these glycoproteins attachment sites for biofilm formation. This study introduces boronic acid-functionalized polyethyleneimine (PEI-BA) promising antibiofilm agent that effectively blocks bacterial adhesion mucin-rich surfaces. Through the multivalent presentation of acid groups, PEI-BA reversibly forms boronate ester bonds with mucin glycans, creating barrier. Our findings show prevents through nonbactericidal mechanism, potentially reducing risk development. Notably, synergizes antibiotic, tobramycin, significantly enhancing inhibition compared either treatment alone. Systematic evaluation formulations identified optimal functionalization levels, balancing glycan-binding capability solubility. From biomaterials design perspective, we demonstrate how rational polymer modification transform potent but cytotoxic (i.e., PEI) into safe and effective material, opening further possibilities managing biofilm-associated infections in clinical settings. work establishes acid-based nanomaterials candidates prevention antibiotic enhancement, particularly conditions like cystic fibrosis, where mucin-bacterial interactions contribute disease progression.

Language: Английский

Citations

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