Enhanced Whole Tumor Cell-Based Vaccines by a RAFT and Protein Fusion Strategy for Tumor Immunotherapy DOI
Yang He,

Ruyan Feng,

Xingyu Heng

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Inactivated whole tumor cell-based vaccines (WTVs) are a promising strategy for immunotherapy, but have exhibited limited antitumor effects clinically. Aiming at constructing enhanced WTVs, we developed glycopolymer-engineered WTVs (G-WTVs) using Halo-Tag protein (HTP) fusion technique and reversible addition-fragmentation chain transfer (RAFT) polymerization. In our study, G-WTVs with varying molecular weights of glycopolymers were constructed. Compared to unmodified cells, all effectively induced the polarization macrophages toward M1 phenotype promoted secretion pro-inflammatory cytokines. This immune response was attributed improved interactions between macrophages. Among G-WTVs, medium weight variant demonstrated most pronounced enhancement responses. Notably, administration optimized inhibited growth B16 melanoma in mice. Our findings provide new approach enhance efficacy via cell membrane glycopolymer engineering, offering immunotherapy.

Language: Английский

Enhanced Whole Tumor Cell-Based Vaccines by a RAFT and Protein Fusion Strategy for Tumor Immunotherapy DOI
Yang He,

Ruyan Feng,

Xingyu Heng

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Inactivated whole tumor cell-based vaccines (WTVs) are a promising strategy for immunotherapy, but have exhibited limited antitumor effects clinically. Aiming at constructing enhanced WTVs, we developed glycopolymer-engineered WTVs (G-WTVs) using Halo-Tag protein (HTP) fusion technique and reversible addition-fragmentation chain transfer (RAFT) polymerization. In our study, G-WTVs with varying molecular weights of glycopolymers were constructed. Compared to unmodified cells, all effectively induced the polarization macrophages toward M1 phenotype promoted secretion pro-inflammatory cytokines. This immune response was attributed improved interactions between macrophages. Among G-WTVs, medium weight variant demonstrated most pronounced enhancement responses. Notably, administration optimized inhibited growth B16 melanoma in mice. Our findings provide new approach enhance efficacy via cell membrane glycopolymer engineering, offering immunotherapy.

Language: Английский

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