Dual-Modality Imaging Unveil Inner Mitochondrial Membrane Viscosity and Respiratory Dynamics in Mitophagy DOI
Fei Peng,

Xiangnan Ai,

Bin Hao

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Mitophagy is a vital lysosome-dependent process that maintains mitochondrial integrity and cellular homeostasis, where respiration inner membrane (IMM) viscosity play key roles. Despite its critical importance, achieving high-resolution dynamic visualization of IMM during mitophagy remains significant challenge. In this study, we designed two innovative fluorescent probes: SiR-C8, viscosity-sensitive rotor-type probe based on silicon-rhodamine, specifically targeting the IMM, OR-ATP, rhodamine-derived utilizing an intramolecular spirolactam structure to respond ATP levels. Leveraging fluorescence intensity lifetime dual-modality imaging, successfully enabled high-resolution, real-time monitoring mitophagy. Remarkably, our results unveiled progressive increase in alongside attenuation induced by starvation, carbonyl cyanide, m-chlorophenyl hydrazone (CCCP), Oligomycin. Significantly, structured illumination microscopy super-resolution have uncovered novel quality control mechanism which lysosomes selectively engulf locally damaged regions. This discovery provides insights into intricate processes governing introduces platform for studying dynamics, dysfunction, their implications homeostasis pathology.

Language: Английский

Dual-Modality Imaging Unveil Inner Mitochondrial Membrane Viscosity and Respiratory Dynamics in Mitophagy DOI
Fei Peng,

Xiangnan Ai,

Bin Hao

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Mitophagy is a vital lysosome-dependent process that maintains mitochondrial integrity and cellular homeostasis, where respiration inner membrane (IMM) viscosity play key roles. Despite its critical importance, achieving high-resolution dynamic visualization of IMM during mitophagy remains significant challenge. In this study, we designed two innovative fluorescent probes: SiR-C8, viscosity-sensitive rotor-type probe based on silicon-rhodamine, specifically targeting the IMM, OR-ATP, rhodamine-derived utilizing an intramolecular spirolactam structure to respond ATP levels. Leveraging fluorescence intensity lifetime dual-modality imaging, successfully enabled high-resolution, real-time monitoring mitophagy. Remarkably, our results unveiled progressive increase in alongside attenuation induced by starvation, carbonyl cyanide, m-chlorophenyl hydrazone (CCCP), Oligomycin. Significantly, structured illumination microscopy super-resolution have uncovered novel quality control mechanism which lysosomes selectively engulf locally damaged regions. This discovery provides insights into intricate processes governing introduces platform for studying dynamics, dysfunction, their implications homeostasis pathology.

Language: Английский

Citations

0