Bioengineered Tumor‐Stroma Prostate Cancer In Vitro Models for Screening Therapeutics DOI Open Access
Maria V. Monteiro, Filipa Moreira‐Silva,

Matilde R. Lagarto

et al.

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

ABSTRACT Cancer‐associated fibroblasts are increasingly recognized to have a high impact on prostate tumor growth and drug resistance. Here, we bioengineered organotypic cancer 3D in vitro models better understand tumor‐stroma interplay, the metabolomic profile underlying such interactions, their standard‐of‐care therapeutics performance. The assembly of robust uniform spheroids was evaluated compared monotypic PC‐3 heterotypic microtumors comprised either healthy or malignant stroma cells. Our findings demonstrate that precise inclusion stromal elements is crucial generating with reproducible morphology size. cancer‐associated promoted establishment more compact exhibiting characteristic expression major proteins. Exometabolomic analysis also highlighted cells metabolism. optimized were additionally exploited for screening therapeutics, higher resistance when counterparts. including use as testing platforms, being relevant candidate anti‐cancer discovery potential combinations emerging anti‐stroma therapies.

Language: Английский

Bioengineered Tumor‐Stroma Prostate Cancer In Vitro Models for Screening Therapeutics DOI Open Access
Maria V. Monteiro, Filipa Moreira‐Silva,

Matilde R. Lagarto

et al.

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

ABSTRACT Cancer‐associated fibroblasts are increasingly recognized to have a high impact on prostate tumor growth and drug resistance. Here, we bioengineered organotypic cancer 3D in vitro models better understand tumor‐stroma interplay, the metabolomic profile underlying such interactions, their standard‐of‐care therapeutics performance. The assembly of robust uniform spheroids was evaluated compared monotypic PC‐3 heterotypic microtumors comprised either healthy or malignant stroma cells. Our findings demonstrate that precise inclusion stromal elements is crucial generating with reproducible morphology size. cancer‐associated promoted establishment more compact exhibiting characteristic expression major proteins. Exometabolomic analysis also highlighted cells metabolism. optimized were additionally exploited for screening therapeutics, higher resistance when counterparts. including use as testing platforms, being relevant candidate anti‐cancer discovery potential combinations emerging anti‐stroma therapies.

Language: Английский

Citations

0