A Dual Nano-Signal Probe-Based Electrochemical Immunosensor for the Simultaneous Detection of Two Biomarkers in Gastric Cancer DOI Creative Commons

Li-Ting Su,

Zhenqing Yang, Hua‐Ping Peng

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 80 - 80

Published: Jan. 31, 2025

Detecting multiple tumor markers is of great importance. It helps in early cancer detection, accurate diagnosis, and monitoring treatment. In this work, gold nanoparticles–toluidine blue–graphene oxide (AuNPs-TB–GO) nanoparticles–carboxyl ferrocene–tungsten disulfide (AuNPs–FMC–WS2) nanocomposites were prepared for labeling Carcinoembryonic antigen (CEA) antibody Carbohydrate 72–4 (CA72-4) antibody, respectively, used as two kinds probes with different electrochemical signals. With the excellent magnetic performance biotin immune beads (IMBs), biofunctional IMBs firmly deposited on glassy carbon electrode (MGCE) surface by applying a constant field, then CEA CA72-4 immobilized avidin–biotin conjugation. The assay was based change detection peak current. Under optimum experimental conditions, linear range two-component immunosensor from 0.01 to 120 ng/mL, low limit 0.003 0.05 35 U/mL, 0.016 U/mL. results showed that proposed enabled simultaneous exhibited good reproducibility, high selectivity, sensitivity. particular, multiplexed immunoassay approach does not require sophisticated fabrication well-suited high-throughput biosensing application other areas.

Language: Английский

Wearable Aptasensors DOI
Navid Rabiee, Mohammad Rabiee

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19160 - 19182

Published: Nov. 27, 2024

This Perspective explores the revolutionary advances in wearable aptasensor (WA) technology, which combines devices and aptamer-based detection systems for personalized, real-time health monitoring. The leverage specificity sensitivity of aptamers to target specific molecules, offering broad applications from continuous glucose tracking early diagnosis diseases. integration data analytics artificial intelligence (AI) allows risk prediction guides preventive measures. While challenges miniaturization, power efficiency, security persist, these hold significant potential democratize healthcare reshape patient-doctor interactions.

Language: Английский

Citations

6
A Dual Nano-Signal Probe-Based Electrochemical Immunosensor for the Simultaneous Detection of Two Biomarkers in Gastric Cancer DOI Creative Commons

Li-Ting Su,

Zhenqing Yang, Hua‐Ping Peng

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 80 - 80

Published: Jan. 31, 2025

Detecting multiple tumor markers is of great importance. It helps in early cancer detection, accurate diagnosis, and monitoring treatment. In this work, gold nanoparticles–toluidine blue–graphene oxide (AuNPs-TB–GO) nanoparticles–carboxyl ferrocene–tungsten disulfide (AuNPs–FMC–WS2) nanocomposites were prepared for labeling Carcinoembryonic antigen (CEA) antibody Carbohydrate 72–4 (CA72-4) antibody, respectively, used as two kinds probes with different electrochemical signals. With the excellent magnetic performance biotin immune beads (IMBs), biofunctional IMBs firmly deposited on glassy carbon electrode (MGCE) surface by applying a constant field, then CEA CA72-4 immobilized avidin–biotin conjugation. The assay was based change detection peak current. Under optimum experimental conditions, linear range two-component immunosensor from 0.01 to 120 ng/mL, low limit 0.003 0.05 35 U/mL, 0.016 U/mL. results showed that proposed enabled simultaneous exhibited good reproducibility, high selectivity, sensitivity. particular, multiplexed immunoassay approach does not require sophisticated fabrication well-suited high-throughput biosensing application other areas.

Language: Английский

Citations

0