The dual effect of Selenium nanoparticles in rice seedlings: From increasing antioxidant activity to inducing oxidative stress

Plant Stress, Journal Year: 2024, Volume and Issue: 11, P. 100372 - 100372

Published: Jan. 26, 2024

Selenium deficiency has been a worldwide concern because it can pose risk to human health. nanoparticles (SeNPs) are in general less toxic and more bioavailable than ionic Se and, therefore, their use for biofortification of food crops attracted attention. Rice is staple consumed by half the world's population. Indeed, studies on SeNPs' impacts oxidative stress antioxidant responses rice seedlings scarce. Herein, effect different concentrations SeNPs growth, enzyme activity, reactive oxygen species, nitric oxide metabolites germinating seeds were investigated. Results revealed dual seedling growth. At low (0.5 mg L−1), did not affect while at higher root elongation was significantly decreased. Soaking with 0.5 L−1 effectively improved activity ascorbate peroxidase, catalase, superoxide dismutase enzymes. However, decrease observed increasing concentrations. All treatments decreased S-nitrosothiols nitrite compared control. Hydrogen peroxide also 5 L−1, Our results suggest beneficial optimal concentration stimulating metabolism mitigating species plant tissues without affecting physiological parameters.

Language: Английский

---

Hyaluronic Acid-Coated Silica Nanoparticles for Targeted Delivery of Nitric Oxide to Cancer Cells

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(6), P. 3796 - 3809

Published: May 22, 2024

Drug resistance and off-target toxicity are two of the greatest challenges to chemotherapeutic melanoma treatments. Nitric oxide (NO) represents an attractive alternative conventional therapeutics due its numerous anticancer properties low probability engendering resistance. As NO is highly reactive, macromolecular donors needed for controlled targeted delivery therapeutic applications. Herein, mesoporous silica nanoparticles (MSNs) coated with hyaluronic acid (HA) were developed as a system facilitate cancer cells through both passive active targeting via enhanced permeation retention effect directed binding HA CD44 receptors, respectively. The aminosilane modification, concentration, molecular weight systematically evaluated MSN coating loading. hydrodynamic diameter dispersity increased after hydrophilic nature HA, greater increases observed at higher weight. Lower starting concentrations aminosilanes longer alkyl chains favored more efficient coating. Faster NO-release kinetics lower payloads HA-coated MSNs relative uncoated MSNs. However, localized conferred by levels oxidative stress induced mitochondria-mediated apoptosis in cells. Cytotoxicity was also against human dermal fibroblasts, use 6 kDa resulting indices. Enhanced internalization into versus visualized confocal microscopy quantified fluorescence spectroscopy. In total, represent promising potential skin melanomas.

Language: Английский

---

Alternative Metal with an Alternative Mechanism for Metalloporphyrin-Enabled NO Reduction to N₂O: A Combined Computational and Experimental Investigation of NO Reduction by Cr Porphyrin with Lewis Acid

Inorganic Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Nitric oxide (NO) is an important molecule that regulates many physiological processes in humans and plants contributes to the formation of greenhouse gases. Bacterial NO reductases utilize a di-Fe heme/nonheme active site couple two NOs generate nitrous (N2O) via two-electron mechanism. Here, we report previously unexplored Cr porphyrin complex with Lewis acid (LA) BF3 for reduction reaction. Density functional theory calculations were first employed reveal its reaction mechanism reasonable barrier experimental realization. Subsequent synthesis work confirms this reactivity reports nitrosyl X-ray crystal structure. Theoretical analysis uncovered distinctive feature system compared Fe Co porphyrins: electron transfer from metal bound occurs before LA binding. A comparative study coupling mechanisms three representative metals suggests potential should be finely tuned, as found previous studies NOR enzymatic systems. Overall, offers new theoretical insights further facilitate development alternative compounds biological, environmental, industrial applications.

Language: Английский

---

Metal-Organic Frameworks: An Overview of a Possible Solution for Modern Agriculture

Microporous and Mesoporous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 113609 - 113609

Published: March 1, 2025

Language: Английский

---

Innovative nitric oxide-releasing nanomaterials: current progress, trends, challenges, and perspectives in cardiovascular therapies

Nitric Oxide, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

---

Nitric oxide-delivering surface modification for tailoring cell-material interactions on medical device surface: From surface-functionalized design to therapeutic applications

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162378 - 162378

Published: April 1, 2025

Language: Английский

---

Nitric Oxide-Photodelivering Materials with Multiple Functionalities: From Rational Design to Therapeutic Applications

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(44), P. 59697 - 59720

Published: Oct. 24, 2024

The achievement of materials that are able to release therapeutic agents under the control light stimuli improve efficacy is a significant challenge in health care. Nitric oxide (NO) one most studied molecules fascinating realm biomedical sciences, not only for its crucial role as gaseous signaling molecule human body but also great potential an unconventional variety diseases including cancer, bacterial and viral infections, neurodegeneration. Handling difficulties due nature, reduced region action short half-life, strict dependence biological effects on concentration generation site critical questions be solved appropriate uses NO. Light-activatable NO precursors, namely, photodonors (NOPDs), address above issues since they stable dark permit noninvasive fashion remote-controlled delivery demand with spatiotemporal precision. Engineering biocompatible NOPDs their combination additional imaging, therapeutic, phototherapeutic components leads intriguing light-responsive multifunctional constructs exhibiting promising applications. This contribution illustrates progress made over last five years achieving engineered nanoparticles, gels, thin films, sharing common feature deliver exclusive visible/near infrared inputs. We will highlight logical design behind fabrication these systems, illustrating applications particular emphasis cancer infections.

Language: Английский

---

Nanoparticle-Based Nitric Oxide Donors: Exploring Their Antimicrobial and Anti-Biofilm Capabilities

Antibiotics, Journal Year: 2024, Volume and Issue: 13(11), P. 1047 - 1047

Published: Nov. 5, 2024

Nitric oxide (NO) is an antimicrobial and anti-biofilm agent with significant potential for combating biofilm-associated infections antibiotic resistance. However, owing to its high reactivity due the possession of a free radical short half-life (1-5 s), practical application NO in clinical settings challenging.

Language: Английский

---

Antimicrobial Effects of Nitric Oxide against Plant Pathogens

ACS Omega, Journal Year: 2024, Volume and Issue: 9(24), P. 26066 - 26074

Published: June 3, 2024

Pathogen infection represents the greatest challenge to agricultural crop production, resulting in significant economic loss. Conventional pesticides are used control such but can result antimicrobial resistance and detrimental effects on plant, environment, human health. Due nitric oxide's (NO) endogenous roles plant immune responses, treatment with exogenous NO an attractive nonpesticide approach for eradicating pathogens. In this work, activity of small-molecule donors varying NO-release kinetics was evaluated against Pseudomonas syringae Botrytis cinerea, two prevalent Intermediate proved be most effective at these pathogens vitro. A selected donor (methyl tris diazeniumdiolate; MD3) capable treating both bacterial leaves fungal tomato fruit without exerting toxicity earthworms. Taken together, results demonstrate potential utilizing as a broad-spectrum, environmentally safe pesticide may guide development other application.

Language: Английский

---

Nitric Oxide-Releasing Topical Treatments for Cutaneous Melanoma

Molecular Pharmaceutics, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Melanoma is an aggressive skin cancer notorious for high levels of drug resistance. Additionally, current treatments such as immunotherapies are often associated with numerous adverse side effects. The use nitric oxide (NO) may represent attractive treatment melanoma due to NO's various anticancer properties, unlikeliness foster resistance, and limited toxicity toward healthy tissues. effects chemical NO donors have been explored previously but understanding the needed characteristics exerting optimal antimelanoma activity. Herein, in vitro therapeutic efficacy three macromolecular donor systems (i.e., cyclodextrin, mesoporous silica nanoparticles, hyaluronic acid) tunable NO-release kinetics was by evaluating permeation along against cells. Cytotoxicity cells dependent on payload not identity or kinetics. In contrast, cytotoxicity primarily influenced donor, cyclodextrin- acid-based having highest indices. both size charge smaller, more neutral resulting greater permeation. A Pluronic F127 organogel optimized delivery a cyclodextrin-based donor. Delivery this manner resulted increased reduced tumor growth vivo model.

Language: Английский

---