Sub-10nm nanoscopic imaging reveals sub-10nm cellular architectures DOI
Guangjie Cui, Do Young Kim, Di Zu

et al.

Published: March 13, 2024

Phase intensity nanoscope (PINE) is a new super-resolution method to further improve the resolution of existing techniques. PINE utilizes an integrated phase-intensity device modulate phase differences between electric field components distinguish nanoprobes within diffraction-limited region. This separation enables continuous imaging without photobleaching. achieved sub-10nm cellular structures through precise localization populations randomly distributed nanorod probes. The distribution localized nanorods forms patterns underlying structures. By defining features from probe and minimizing distances each its feature projection, extracts structural information. will pave way for long-term investigations previously unexplored material, chemical biological dynamics.

Language: Английский

Two-color interferometric scattering (iSCAT) microscopy reveals structural dynamics in discrete plasmonic molecules DOI Creative Commons

Leslie Velasco,

Aniqa N. Islam,

Koustav Kundu

et al.

Nanoscale, Journal Year: 2024, Volume and Issue: 16(24), P. 11696 - 11704

Published: Jan. 1, 2024

Plasmonic molecules are discrete assemblies of noble metal nanoparticles (NPs) that interest as transducers in optical nanosensors. So far, NPs with diameters ∼40 nm have been the preferred building blocks for plasmonic intended single molecule sensors due to difficulties associated detecting smaller through elastic scattering conventional darkfield microscopy. Here, we apply 405 nm, 445 two-color interferometric (iSCAT) microscopy characterize polyethylene glycol (PEG) tethered dimers 10 and 20 Ag their monomers. Dimers both NP sizes can be discerned from respective monomers changes average iSCAT contrast. In case NPs, dimer formation induces a change sign contrast, providing characteristic signal binding events. 0.4 kDa 3.4 spacers show contrast distributions significantly different averages on wavelength channels. The measured individual PEG-tethered or function time shows fluctuations indicative rich structural dynamics assembled molecules, which provides an additional metric discern paves path new class plasmon rulers.

Language: Английский

Citations

2
Long-time nanoscopic bioimaging by phase-intensity nanoscopy DOI
Guangjie Cui, Yunbo Liu, Di Zu

et al.

Published: March 12, 2024

Light-matter interactions play essential roles in realizing a new generation of nanoscale imaging to overcome traditional trade-offs between spatial resolution and time capabilities. By harnessing engineered nanophotonic materials, scattered light can be shaped enhance matter for bioimaging. We achieved bioimaging with record long Long nanoscopy has the potential unlock wide range dynamical studies insights assembly, organization pattern formation biological material systems, where nanoscopic rearrangements drive group-level movements at macroscale over time.

Language: Английский

Citations

0
Interferometric phase-intensity nanoscopy DOI
Do Young Kim, Guangjie Cui, Somin Eunice Lee

et al.

Published: March 13, 2024

We present a novel approach, interferometric phase intensity nanoscopy, to clearly define nanometer-scale objects situated closely together, going beyond the traditional diffraction limits. Our approach capitalizes on strengths of scattering, which we've found remarkably effective for detecting entities as minute individual nanoparticles and proteins. integrated multiphase analysis with enhanced sensitivity reveal elliptical Airy patterns that directly correlate nanostructures we observe. A key aspect our methodology is use circular polarized illumination, essential differentiating are spaced below limit. This technique opens up new possibilities prolonged observation nanoscale dynamics in biology, biomedicine, bioengineering.

Language: Английский

Citations

0
Noninvasive nanoimaging-nanomanipulation of CRISPR-Cas in cells DOI
Di Zu,

Borui Li,

Somin Eunice Lee

et al.

Published: March 13, 2024

CRISPR-Cas9 is significantly potential and versatile gene-editing treatment for neurodegenerative disorders. The system incorporates a single guide RNA (sgRNA) Cas9 nuclease, which helps to bind the target sequence, makes double-strand break respectively. Viral vectors, as traditional delivery of CRISPRCas9, cause safety issue regrading immunogenic complications. In this work we present alternative non-viral vectors with plasmonic properties system. Our provides new perspective nanoparticles visualization applications

Language: Английский

Citations

0
Sub-10nm nanoscopic imaging reveals sub-10nm cellular architectures DOI
Guangjie Cui, Do Young Kim, Di Zu

et al.

Published: March 13, 2024

Phase intensity nanoscope (PINE) is a new super-resolution method to further improve the resolution of existing techniques. PINE utilizes an integrated phase-intensity device modulate phase differences between electric field components distinguish nanoprobes within diffraction-limited region. This separation enables continuous imaging without photobleaching. achieved sub-10nm cellular structures through precise localization populations randomly distributed nanorod probes. The distribution localized nanorods forms patterns underlying structures. By defining features from probe and minimizing distances each its feature projection, extracts structural information. will pave way for long-term investigations previously unexplored material, chemical biological dynamics.

Language: Английский

Citations

0