Recent progress and challenges in biosensing of carcinoembryonic antigen

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 180, P. 117964 - 117964

Published: Sept. 7, 2024

Language: Английский

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Label-Free Electrochemical Cell-Based Biosensor for Toxicity Assay of Water-Soluble Form of Phosphatidylcholine

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 996 - 996

Published: April 20, 2025

Background/Objectives: Our study brings a new method to properly evaluating drug efficacy at the non-invasive in vitro level. Methods: In this work, electrochemical mediator-free and reagent-free analysis of cell lines based on registration profiles membrane proteins was developed. We studied specificity Wi-38 HepG2 toxic effects drugs cell-on-electrode systems. Results: A linear dependence peak current concentration cells applied electrode range from 1 × 105 6 cells/electrode registered (R2 0.932 for R2 0.912 HepG2). The water-soluble form phosphatidylcholine (wPC) nanoparticles recommended atherosclerosis treatment prevention cardiovascular diseases did not show effect human fibroblast cells, Wi-38, or hepatocellular carcinoma HepG2, sufficiently high concentrations (such as 0.1–1 mg/mL). antitumor doxorubicin, 3 10 μg/mL, showed pronounced tested lines, where percentage living 50–55%. Conclusions: comparative cytotoxicity wPC (0.1–1 mg/mL) doxorubicin (3–10 μg/mL) using MTT test approach their clear adequacy.

Language: Английский

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CRISPR Diagnostics for WHO High-Priority Sexually Transmitted Infections

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118054 - 118054

Published: Nov. 1, 2024

Language: Английский

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MIP-Modified Porous Silicon Optical Sensor for Interleukin-6 Label-Free Quantification

Biosensors, Journal Year: 2025, Volume and Issue: 15(5), P. 320 - 320

Published: May 17, 2025

In this study, we present an innovative optical biosensor designed to detect Interleukin-6 (IL-6), a pivotal cytokine implicated in many pathological conditions. Our sensing platform is made of porous silicon (PSi) nanostructured substrate modified with thin (~5 nm) molecularly imprinted polymer (MIP), ensuring both high specificity and sensitivity toward IL-6 molecules. The fabrication process involves electrochemical etching chips create the structure, followed by electrodeposition MIP, which tailored selectively bind target. Extensive testing over broad concentration range demonstrates clear, proportional response, yielding limit detection (LOD) 13 nM. Moreover, robustness was verified evaluating its performance bovine serum, complex biological matrix. Despite presence various interfering components, sensor maintained selectivity displayed minimal matrix effects, underlining practical applicability real-world diagnostic scenarios.

Language: Английский

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Development of a label-free electrochemical immunosensor based on self-assembled peptide-templated Au/Cu bimetallic nanozymes for sensitive detection of carcinoembryonic antigen (CEA)

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(6)

Published: May 26, 2025

Language: Английский

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Electrochemical biosensor based on Novozym 435 enzymatic ring-opening polymerization for CEA detection

Journal of Electroanalytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118846 - 118846

Published: Dec. 1, 2024

Language: Английский

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State-of-the-Art Signal Amplification Strategies for Nucleic Acid and Non-Nucleic Acid Biosensors

Sensors and Actuators Reports, Journal Year: 2024, Volume and Issue: unknown, P. 100268 - 100268

Published: Dec. 1, 2024

Language: Английский

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