Mesoporous Pd@Pt Nanoparticle Label/Lateral Flow Immunoassay Integrated with a 3D-Printed Smartphone Reader for Detection of Wood Smoke Biomarkers DOI

Zhansen Yang,

Xinyi Li,

Yonghao Fu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Wood smoke exposure poses significant health risks, particularly in occupations such as firefighting, where short-term to high levels of pollutants is common. The biomonitoring wood smoke-associated biomarkers crucial for assessing human exposure. S-phenylmercapturic acid (S-PMA), a key metabolite benzene, has been widely used reliable biomarker this purpose. However, current S-PMA detection methods lack the speed, portability, and user-friendliness required widespread, on-site applications. In study, we propose novel system that integrates mesoporous Pd@Pt nanoparticle-mediated lateral flow immunoassay (LFIA) with 3D-printed smartphone-based reader detecting S-PMA. Performance testing phosphate-buffered saline spiked urine samples yielded limits 0.5 ng/mL 2.5 ng/mL, respectively. use nanoparticles signal amplifiers LFIA, along integration device accurate image acquisition, significantly enhanced system's sensitivity, achieving well below threshold recommended by American Conference Governmental Industrial Hygienists. With remarkable stability reproducibility, our method provides noninvasive, highly sensitive, rapid, portable, low-cost, user-friendly solution assessment occupational settings, laying foundation future innovations real-time environmental monitoring.

Language: Английский

Mesoporous Pd@Pt Nanoparticle Label/Lateral Flow Immunoassay Integrated with a 3D-Printed Smartphone Reader for Detection of Wood Smoke Biomarkers DOI

Zhansen Yang,

Xinyi Li,

Yonghao Fu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Wood smoke exposure poses significant health risks, particularly in occupations such as firefighting, where short-term to high levels of pollutants is common. The biomonitoring wood smoke-associated biomarkers crucial for assessing human exposure. S-phenylmercapturic acid (S-PMA), a key metabolite benzene, has been widely used reliable biomarker this purpose. However, current S-PMA detection methods lack the speed, portability, and user-friendliness required widespread, on-site applications. In study, we propose novel system that integrates mesoporous Pd@Pt nanoparticle-mediated lateral flow immunoassay (LFIA) with 3D-printed smartphone-based reader detecting S-PMA. Performance testing phosphate-buffered saline spiked urine samples yielded limits 0.5 ng/mL 2.5 ng/mL, respectively. use nanoparticles signal amplifiers LFIA, along integration device accurate image acquisition, significantly enhanced system's sensitivity, achieving well below threshold recommended by American Conference Governmental Industrial Hygienists. With remarkable stability reproducibility, our method provides noninvasive, highly sensitive, rapid, portable, low-cost, user-friendly solution assessment occupational settings, laying foundation future innovations real-time environmental monitoring.

Language: Английский

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