Decellularized Scaffolds for Tissue Regeneration: Techniques and Applications DOI Creative Commons
Esmaeil Biazar

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Regenerative medicine has shown good potential in regenerating tissues and organs. Tissue engineering, as a sub-branch of this technology, helps the process tissue organ regeneration by using enginenering techniques. Biomaterials play pivotal role success engineering. Of course, challenges such origin biomaterials, synthesis extraction, biocompatibility cell adhesion, scaffold design techniques, physical, chemical, mechanical properties scaffolds designed with synthetic biomaterials can create limitations inlimit their use design. A promising option for designing is extracellular matrix (ECM). By removing cellular factors inflammatory factors, unique microenvironment called decellularized (dECM) prepared, which regulates directs processes. In chapter, we discuss various decellularization techniques to preserve structural, physicochemical, biological dECM products some its applications regeneration.

Language: Английский

Biofabrication of anisotropic articular cartilage based on decellularized extracellular matrix DOI Creative Commons
Anna Puiggalı́-Jou, I.K. Hui,

Lucrezia Baldi

et al.

Biofabrication, Journal Year: 2025, Volume and Issue: 17(1), P. 015044 - 015044

Published: Jan. 1, 2025

Tissue-engineered grafts that mimic articular cartilage show promise for treating injuries. However, engineering cell-based therapies to match zonal architecture and biochemical composition remains challenging. Decellularized extracellular matrix (dECM) has gained attention its chondro-inductive properties, yet dECM-based bioinks have limitations in mechanical stability printability. This study proposes a rapid light-based bioprinting method using tyrosine-based crosslinking mechanism, which does not require chemical modifications of dECM thereby preserves structure bioactivity. Combining this resin with Filamented Light (FLight) biofabrication enables the creation cellular, porous, anisotropic scaffolds composed aligned microfilaments. Specifically, we focus on effects various biopolymer compositions (i.e. hyaluronic acid, collagen I, dECM) inner bulk light vs FLight) immune response cell morphology, investigate their influence nascent ECM production long-term tissue maturation. Our findings highlight importance FLight directing deposition resembling promoting construct maturation, they emphasize superiority biological-rich over single-component materials cartilage, offering new avenues development effective strategies.

Language: Английский

Citations

2
Immunomodulatory Bioadhesive Technologies DOI Creative Commons
Xiaoyi Lan,

Eleanor Johnston,

Tianqin Ning

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123274 - 123274

Published: March 1, 2025

Bioadhesives have found significant use in medicine and engineering, particularly for wound care, tissue surgical applications. Compared to traditional closure methods such as sutures staples, bioadhesives offer advantages, including reduced damage, enhanced healing, ease of implementation. Recent progress highlights the synergy immunoengineering strategies, leading immunomodulatory capable modulating immune responses at local sites where are applied. They foster favorable therapeutic outcomes inflammation wounds implants or improve cancer therapy efficacy. The dual functionalities bioadhesion immunomodulation benefit management, regeneration, implantable medical devices, post-surgical management. This review delves into interplay between immunomodulation, highlighting mechanobiological coupling involved. Key areas focus include modulation through chemical physical well application these healing treatment. Discussed remaining challenges achieving long-term stability effectiveness, necessitating further research fully harness clinical potential bioadhesives.

Language: Английский

Citations

0
Light-Activated Decellularized Extracellular Matrix-Based Bioinks for Enhanced Mechanical Integrity DOI Creative Commons
Minji Kim, Dayoon Kang, Hohyeon Han

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101859 - 101859

Published: May 1, 2025

Language: Английский

Citations

0
Biomimetic multizonal scaffolds for the reconstruction of zonal articular cartilage in chondral and osteochondral defects DOI Creative Commons
Xiaoqi Lin, Ye Zhang, Jiarong Li

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 43, P. 510 - 549

Published: Oct. 11, 2024

Language: Английский

Citations

3
Anisotropic Articular Cartilage Biofabrication based on Decellularized Extracellular Matrix DOI Open Access
Anna Puiggalı́-Jou, I.K. Hui,

Lucrezia Baldi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 30, 2024

Tissue-engineered grafts that mimic articular cartilage show promise for treating injuries. However, engineering cell-based therapies to match zonal architecture and biochemical composition remains challenging. Decellularized extracellular matrix (dECM) has gained attention its chondro-inductive properties, yet dECM-based bioinks have limitations in mechanical stability printability. This study proposes a rapid light-based bioprinting method using tyrosine-based crosslinking mechanism, which does not require chemical modifications of dECM thereby preserves structure bioactivity. Combining this resin with Filamented Light (FLight) biofabrication enables the creation cellular, porous, anisotropic scaffolds composed aligned microfilaments. Specifically, we investigate effects various biopolymer compositions (i.e., hyaluronic acid, collagen I, dECM) inner bulk light vs FLight) on immune response cell morphology, their influence nascent ECM production long-term tissue maturation. Our findings highlight importance FLight directing deposition resembling promoting construct maturation, they emphasize superiority biological-rich over single-component materials cartilage, offering new avenues development effective strategies.

Language: Английский

Citations

0
Decellularized Scaffolds for Tissue Regeneration: Techniques and Applications DOI Creative Commons
Esmaeil Biazar

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Regenerative medicine has shown good potential in regenerating tissues and organs. Tissue engineering, as a sub-branch of this technology, helps the process tissue organ regeneration by using enginenering techniques. Biomaterials play pivotal role success engineering. Of course, challenges such origin biomaterials, synthesis extraction, biocompatibility cell adhesion, scaffold design techniques, physical, chemical, mechanical properties scaffolds designed with synthetic biomaterials can create limitations inlimit their use design. A promising option for designing is extracellular matrix (ECM). By removing cellular factors inflammatory factors, unique microenvironment called decellularized (dECM) prepared, which regulates directs processes. In chapter, we discuss various decellularization techniques to preserve structural, physicochemical, biological dECM products some its applications regeneration.

Language: Английский

Citations

0