Loss of PA28γ exacerbates imbalanced differentiation of bone marrow stromal cells during bone formation and bone healing in mice
Journal of Bone and Mineral Research,
Journal Year:
2024,
Volume and Issue:
39(3), P. 326 - 340
Published: Jan. 31, 2024
Proteasome
activator
subunit
3
(PA28γ)
is
a
member
of
the
proteasome
family,
which
mainly
regulates
degradation
and
stability
proteins.
Studies
have
shown
that
it
plays
crucial
roles
in
lipid
formation,
stemness
maintenance,
blood
vessel
formation.
However,
few
studies
clarified
association
between
PA28γ
bone
diseases.
Herein,
we
identified
as
previously
unknown
regulator
homeostasis
coordinates
formation
accumulation.
PA28γ-knockout
mice
presented
with
characteristics
low
mass
accumulation
lipids.
Suppressed
expression
restrained
osteogenic
differentiation
enhanced
adipogenic
marrow
stromal
cells
(BMSCs).
Overexpression
promoted
inhibited
BMSCs.
Mechanistically,
interacted
Wnt5α,
two
interactors
appeared
to
be
positively
correlated.
activated
downstream
Wnt/β-catenin
signaling
pathway,
affects
BMSCs
homeostasis.
Deletion
Wnt5α
significantly
delayed
promotion
partially
alleviated
inhibitory
effect
PA28γ-overexpressing
group.
Furthermore,
demonstrated
had
an
rate
healing
drill-hole
femoral
defect
model
vivo.
Therefore,
our
results
confirm
effects
on
repair,
indicating
interacts
activate
pathway
regulating
Our
reveal
function
diseases
provide
new
theoretical
basis
for
expanding
treatment
Language: Английский
Investigation on the Microstructural Diversity of a Three-Dimensional Porous Hydroxyapatite/Wollastonite Skeleton via Biomineralization in Simulated Body Fluids
Bin Jiang,
No information about this author
Xin Li,
No information about this author
Bozhi Yang
No information about this author
et al.
Applied Sciences,
Journal Year:
2023,
Volume and Issue:
13(20), P. 11593 - 11593
Published: Oct. 23, 2023
The
occurrence
of
fractures
has
emerged
as
one
the
most
prevalent
injuries
in
human
body.
In
bone
reconstruction
surgery,
after
implantation
porous
hydroxyapatite
materials,
there
is
an
initial
infiltration
body
fluids
into
implant,
followed
by
biomineralization-mediated
apatite
crystal
formation
and
subsequent
ingrowth
cells.
Despite
extensive
research
efforts
this
field,
previous
investigations
have
primarily
focused
on
crystals
exposed
surfaces,
with
limited
literature
available
regarding
within
internal
microstructures
implants.
Herein,
we
demonstrate
dynamic
biomineralization
a
three-dimensional
hydroxyapatite/wollastonite
(HA/WS)
skeleton,
leading
to
abundant
nano-sized
across
diverse
environments.
Our
findings
reveal
that
these
nanocrystals
distinct
rates
nucleation,
packing
densities,
forms
comparison
those
formed
surface.
Therefore,
objective
study
was
elucidate
temporal
evolution
processes
investigating
surfaces
HA/WS
materials
at
stages
subsequently
explore
biological
activity
exhibited
when
combined
cell
investigation
mechanisms
nanoscale,
aiming
comprehend
natural
develop
efficacious
biomimetic
implants
for
tissue
engineering
applications.
simultaneous
examination
attachment
its
interaction
ongoing
nanocrystal
will
provide
valuable
insights
designing
optimal
scaffolds
conducive
growth,
which
imperative
endeavors.
Language: Английский