Loss of PA28γ exacerbates imbalanced differentiation of bone marrow stromal cells during bone formation and bone healing in mice

Journal of Bone and Mineral Research, Journal Year: 2024, Volume and Issue: 39(3), P. 326 - 340

Published: Jan. 31, 2024

Proteasome activator subunit 3 (PA28γ) is a member of the proteasome family, which mainly regulates degradation and stability proteins. Studies have shown that it plays crucial roles in lipid formation, stemness maintenance, blood vessel formation. However, few studies clarified association between PA28γ bone diseases. Herein, we identified as previously unknown regulator homeostasis coordinates formation accumulation. PA28γ-knockout mice presented with characteristics low mass accumulation lipids. Suppressed expression restrained osteogenic differentiation enhanced adipogenic marrow stromal cells (BMSCs). Overexpression promoted inhibited BMSCs. Mechanistically, interacted Wnt5α, two interactors appeared to be positively correlated. activated downstream Wnt/β-catenin signaling pathway, affects BMSCs homeostasis. Deletion Wnt5α significantly delayed promotion partially alleviated inhibitory effect PA28γ-overexpressing group. Furthermore, demonstrated had an rate healing drill-hole femoral defect model vivo. Therefore, our results confirm effects on repair, indicating interacts activate pathway regulating Our reveal function diseases provide new theoretical basis for expanding treatment

Language: Английский

Investigation on the Microstructural Diversity of a Three-Dimensional Porous Hydroxyapatite/Wollastonite Skeleton via Biomineralization in Simulated Body Fluids

Applied Sciences, Journal Year: 2023, Volume and Issue: 13(20), P. 11593 - 11593

Published: Oct. 23, 2023

The occurrence of fractures has emerged as one the most prevalent injuries in human body. In bone reconstruction surgery, after implantation porous hydroxyapatite materials, there is an initial infiltration body fluids into implant, followed by biomineralization-mediated apatite crystal formation and subsequent ingrowth cells. Despite extensive research efforts this field, previous investigations have primarily focused on crystals exposed surfaces, with limited literature available regarding within internal microstructures implants. Herein, we demonstrate dynamic biomineralization a three-dimensional hydroxyapatite/wollastonite (HA/WS) skeleton, leading to abundant nano-sized across diverse environments. Our findings reveal that these nanocrystals distinct rates nucleation, packing densities, forms comparison those formed surface. Therefore, objective study was elucidate temporal evolution processes investigating surfaces HA/WS materials at stages subsequently explore biological activity exhibited when combined cell investigation mechanisms nanoscale, aiming comprehend natural develop efficacious biomimetic implants for tissue engineering applications. simultaneous examination attachment its interaction ongoing nanocrystal will provide valuable insights designing optimal scaffolds conducive growth, which imperative endeavors.

Language: Английский