3D Extrusion-Printed Alginate–Gelatin Hydrogel Modified with Nanoscale Hydroxyapatite: A Comprehensive Understanding of Process Science and Evaluation of the Antimicrobial Property DOI Creative Commons
Sulob Roy Chowdhury, K. Dey, Bikramjit Basu

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

In developing hydrogel scaffolds for the soft tissue regeneration, a number of inorganic or carbonaceous fillers are embedded in alginate/gelatin-based while manufacturing shape fidelity compliant constructs using three-dimensional (3D) extrusion printing. Among spectrum nanofillers, nanohydroxyapatite (nHAP), due to its intrinsic bioactivity, could promote mineralization and interaction with host tissues conferring superior mechanical properties (strength elastic modulus). Against this backdrop, study demonstrates effectiveness nHAP reinforcement tuning several clinically relevant such as rheological properties, swelling, degradation, antimicrobial properties. At higher concentrations (0.75%) matrix (3A5G0.75H), 3.13-fold increment compressive strength was observed, gel stability window thermal cross-linked graft being extended greater than 40 143 °C, respectively. This demonstrated printability nHAP-reinforced ink by fabricating matrix-shaped dimension 20 mm diameter 10 thickness, buildability established making bulk-sized construct up 62 layers (20 height) well-maintained pore interconnectivity, micro-CT analysis. Interestingly, CFU revealed 2.9- 1.5-fold improvement reduction bacterial adhesion 3A5G0.75H respect Escherichia coli Staphylococcus aureus bacteria. Cell culture studies on 3D printed w.r.to NIH 3T3 fibroblast cell line consistent increase viability pronounced filopodial extensions, confirming cytocompatibility their ability support cellular growth during an vitro culture. Taken together, present uncovers process science-based understanding biophysical different inks

Language: Английский

3D Printing of a Self-Healing, Bioactive, and Dual-Cross-Linked Polysaccharide-Based Composite Hydrogel as a Scaffold for Bone Tissue Engineering DOI
Saba Nasiripour, Fatemehsadat Pishbin, S.A. Seyyed Ebrahimi

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Although 3D printing is becoming a dominant technique for scaffold preparation in bone tissue engineering (TE), developing hydrogel-based ink compositions with bioactive and self-healing properties remains challenge. This research focuses on based composite hydrogel, which maintains its after incorporating glass 3D-printable. The plain hydrogel was synthesized using natural polymers of 1 wt % N-carboxyethyl chitosan, 2 hyaluronic acid aldehyde, 0.3 adipic hydrazide, alginate (ALG) (2, 5, 10 %). Bioactive (BG) (0 5 w/v %) particles were incorporated into the matrix to obtain an osteogenic hydrogel. material characterized via rheology, field emission scanning electron microscopy/energy-dispersive X-ray spectroscopy (FESEM/EDS), Fourier transform infrared (FTIR) spectroscopy, diffraction (XRD), swelling, degradation, bioactivity, vitro cellular assessments. Rheological evaluations confirmed that specimen 0 BG ALG exhibited highest G', G″, viscosity values. All specimens self-healing, provided by two reversible dynamic bonds, namely, imine acylhydrazone. Bioactivity evaluation SBF immersion revealed formation HA hydrogels. MTT cytotoxicity assay MG63 indicated sample containing had cell viability (95 ± 1.02%) culture day 3. developed approach presents promising formulation high potential extrusion-based TE constructs.

Language: Английский

Citations

1
3D bioprinted GelMA scaffolds for clinical applications: promise and challenges DOI
Soumitra Das, Remya Valoor, Jeyapriya Thimukonda Jegadeesan

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: unknown, P. e00365 - e00365

Published: Oct. 1, 2024

Language: Английский

Citations

4
High-strength and excellent biological activity of modified tricalcium silicate/bioglass composite bone cement for biomaterials DOI
Yin Zhang, Zilong Zhang,

Qiuli Fang

et al.

Ceramics International, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Phase-adapted Metal Ion Supply for Spinal Cord Repair with a Mg-Zn Incorporated Chimeric Microsphere DOI
Xiangyu Liu,

Biao Ma,

S L Hu

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: 320, P. 123253 - 123253

Published: March 11, 2025

Language: Английский

Citations

0
Dual Crosslinkable Gelatin Glycidyl Methacrylate-based Hydrogel: Key Insights to Biophysical Properties and Applicability Towards 3D Printing DOI

Garga Mondal,

Sulob Roy Chowdhury,

Priyanka Paul

et al.

Regenerative Engineering and Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Language: Английский

Citations

0
3D Extrusion-Printed Alginate–Gelatin Hydrogel Modified with Nanoscale Hydroxyapatite: A Comprehensive Understanding of Process Science and Evaluation of the Antimicrobial Property DOI Creative Commons
Sulob Roy Chowdhury, K. Dey, Bikramjit Basu

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

In developing hydrogel scaffolds for the soft tissue regeneration, a number of inorganic or carbonaceous fillers are embedded in alginate/gelatin-based while manufacturing shape fidelity compliant constructs using three-dimensional (3D) extrusion printing. Among spectrum nanofillers, nanohydroxyapatite (nHAP), due to its intrinsic bioactivity, could promote mineralization and interaction with host tissues conferring superior mechanical properties (strength elastic modulus). Against this backdrop, study demonstrates effectiveness nHAP reinforcement tuning several clinically relevant such as rheological properties, swelling, degradation, antimicrobial properties. At higher concentrations (0.75%) matrix (3A5G0.75H), 3.13-fold increment compressive strength was observed, gel stability window thermal cross-linked graft being extended greater than 40 143 °C, respectively. This demonstrated printability nHAP-reinforced ink by fabricating matrix-shaped dimension 20 mm diameter 10 thickness, buildability established making bulk-sized construct up 62 layers (20 height) well-maintained pore interconnectivity, micro-CT analysis. Interestingly, CFU revealed 2.9- 1.5-fold improvement reduction bacterial adhesion 3A5G0.75H respect Escherichia coli Staphylococcus aureus bacteria. Cell culture studies on 3D printed w.r.to NIH 3T3 fibroblast cell line consistent increase viability pronounced filopodial extensions, confirming cytocompatibility their ability support cellular growth during an vitro culture. Taken together, present uncovers process science-based understanding biophysical different inks

Language: Английский

Citations

0