Synergistic hybrid nanostructures for Ultra-Sensitive photoelectrochemical detection of hepatocellular carcinoma ctDNA DOI
Meltem Okan, Zeynep Çağlayan Arslan, Firdevs Aydın

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 112547 - 112547

Published: Dec. 1, 2024

Language: Английский

Liquid Biopsies in the Early Diagnosis, Prognosis, and Tailored Treatment of Colorectal Cancer DOI Open Access
Εfstathia Liatsou,

Ioannis Kollias,

Maria Trapali

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(6), P. 927 - 927

Published: March 8, 2025

Introduction: Liquid biopsies provide a less-invasive option to tissue for the early diagnosis, prognosis, and tailored therapy of colorectal cancer (CRC). CRC is major cause cancer-related death, identification essential improving patient outcomes. Review: Conventional diagnostic techniques, including colonoscopy biopsy, may be enhanced by liquid that examine circulating tumor cells (CTCs), DNA (ctDNA), extracellular vesicles (EVs), other indicators present in body fluids. These markers significant insights into biology, heterogeneity, therapeutic response. CTCs detected early-stage have prognostic significance disease recurrence survival, while ctDNA investigation uncover genetic mutations, epigenetic alterations, development. The minimal residual (MRD) postsurgery correlates with an elevated risk unfavorable underscoring its use assessing treatment effectiveness. Furthermore, non-coding RNAs (ncRNAs) contained inside EVs potential prospective biomarkers targets, facilitating diagnosis assessment. Notwithstanding biopsies, obstacles persist assay standardization, sensitivity enhancement, management heterogeneity. Additional extensive research required determine their function clinical practice. Conclusion: Overall, serve as instrument real-time monitoring, evaluating responses, directing individualized strategies patients.

Language: Английский

Citations

0
Recent advances in DNA nanotechnology for cancer detection and therapy: A review DOI

Donya Esmaeilpour,

Matineh Ghomi, Ehsan Nazarzadeh Zare‬

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142136 - 142136

Published: March 1, 2025

Language: Английский

Citations

0
Immuno-PCR: A high-sensitivity approach for biomarker analysis DOI

Hansen Shi,

Hansen Shi,

Biyun Zeng

et al.

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 120289 - 120289

Published: April 1, 2025

Language: Английский

Citations

0
CRISPR/Cas12a Corona Nanomachine for Detecting Circulating Tumor Nucleic Acids in Serum DOI

Aijiao Yuan,

T. R. Sun, Leyuan Chen

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

Circulating tumor nucleic acids (CTNAs), which consist of cell-free DNA or RNA released from cells, are utilized as potential biomarkers for diagnosing and managing prognosis. There is a significant demand developing highly sensitive reliable assay CTNAs detection. In this study, we engineered CRISPR/Cas12a corona nanomachine capable detecting circulating in serum. This consists protein shell incorporating Cas12a/crRNA ribonucleoprotein complexes scaffold AuNP core decorated with substrate ssDNA strands. The protective CRISPR shields the acid degradation by nuclease DNase/RNase, thereby enhancing stability biological fluids, even tolerating up to undiluted human serum FBS. Upon encountering target CTNAs, activated through sequence-specific hybridization between crRNA CTNAs. Subsequently, autonomously cleaves collateral substrates on AuNPs, releasing fluorophore-labeled fragment generating an increasing fluorescent signal. was successfully employed detect various including (ct)DNA/RNA (EGFR L858R) microRNA-21, achieving limit detection 0.14 pM ctDNA 1.0 RNA. enables simultaneous both complex samples, offering promising tool early diagnosis.

Language: Английский

Citations

3
Pop-Up Paper-Based Biosensor for a Dual-Mode Lung Cancer ctDNA Assay Based on Novel CoB Nanosheets with Dual-Enzyme Activities and a Portable Smartphone/Barometer for Readout DOI
Siyi Yang,

Zhu Jia-jia,

Liyu Yang

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Timely monitoring of circulating tumor DNA (ctDNA) in serum is meaningful for personalized diagnosis and treatment lung cancer. Cheap efficient point-of-care testing (POCT) has emerged as a promising method, especially low-resource setting. Herein, (i) 3D pop-up paper-based POCT device was designed manufactured via cheap method; it used saving time efficiently building biosensor; (ii) novel cobalt boride nanosheet (CoB NS) nanozyme with abundant groups dual-mode signal transduction then portable smartphone/pressure meter to readout; (iii) user-friendly smartphone app fabricated achieving more convenient POCT. Detailly, the generated based on CoB NS peroxidase activity catalyze chromogenic agent develop color catalase decomposition H

Language: Английский

Citations

1
Synergistic hybrid nanostructures for Ultra-Sensitive photoelectrochemical detection of hepatocellular carcinoma ctDNA DOI
Meltem Okan, Zeynep Çağlayan Arslan, Firdevs Aydın

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 112547 - 112547

Published: Dec. 1, 2024

Language: Английский

Citations

0