Harnessing Theraoenergetics for Cartilage Regeneration: Development of a Therapeutic and Bioenergetic Loaded Janus Nanofiber Reinforced Hydrogel Composite for Cartilage Regeneration
ACS Biomaterials Science & Engineering,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 3, 2025
Advancements
in
tissue
engineering
and
regenerative
medicine
have
highlighted
different
strategies
of
designing
hydrogels
to
replicate
the
intricate
structure
cartilage
extracellular
matrix
(ECM)
for
effective
regeneration.
However,
despite
efforts
meet
elevated
structural
mechanical
demands
repair,
researchers
often
overlook
challenging
environmental
conditions
at
damaged
sites
such
as
inflammation,
hypoxia,
limited
availability
nutrients
energy,
which
are
critical
supporting
The
insufficient
oxygen,
nutrient
availability,
oxidative
stress
avascular
limit
phosphorylation-mediated
bioenergetics
cells
needed
energy
required
anabolic
biosynthesis,
cell
division,
migration
during
repair.
Thus,
there
is
a
need
develop
an
advanced
approach
engineer
unique
hydrogel
system
that
not
only
provides
properties
but
also
integrates
therapeutics
(like
anti-inflammatory,
reactive
oxygen
species
(ROS)
scavenging)
demand)
into
hydrogel,
may
offer
holistic
solution
repairing
defects
under
harsh
microenvironment.
In
this
study,
we
engineered
innovative
new
class
theraoenergetic
by
reinforcing
Janus
nanofiber
(JNF)
carrying
therapeutic
(MgO)
bioenergetic
(polyglutamic
acid),
PGA)
components
dual
network
photo-crosslinkable
hydrogel.
Reinforcement
JNF
microfragments
photo-crosslinking
synthesized
gelatin
methacryloyl
(GelMA)
carboxymethyl
chitosan
(CMCh)
enhances
hydrogel's
800%
withstand
load
ensures
controlled
release
magnesium,
PGA
over
30
days.
Co-delivery
magnesium
with
helped
synergistically
reduce
intracellular
ROS
inflammatory
markers
IL-6
TNF-α,
providing
supportive
environment
enhancing
mitochondrial
metabolism
leading
active
proliferation
chondrogenic
differentiation
stem
deposit
glycosaminoglycan
(GAG)-rich
regenerate
cartilage.
developed
represents
promising
regenerating
microenvironment
treat
osteoarthritis,
rising
global
health
burden.
Language: Английский
The Influence of Cuprorivaite Nanoparticles on the Physicomechanical and Biological Performance of 3D‐Printed Scaffold Based on Carboxymethyl Chitosan Combined With Zein for Bone Tissue Engineering
Mojtaba Ansari,
No information about this author
Hossein Eslami,
No information about this author
Afsaneh Karimi
No information about this author
et al.
Biopolymers,
Journal Year:
2025,
Volume and Issue:
116(2)
Published: Feb. 13, 2025
This
study
demonstrates
a
new
degradable
3D-printed
carboxymethyl
chitosan
(CMC)/zein
bone
scaffold
loaded
with
different
content
of
cuprorivaite
(Cup)
nanoparticles
which
labeled
as
CMCS/Z/Cup.
Only
few
studies
have
utilized
these
components
to
fabricate
three-component
porous
osteogenic
scaffold.
The
aim
this
was
comprehensively
assess
the
mechanical
and
biocompatibility
nanocomposite
synthesized
by
3D
printing
method.
For
purpose,
Cup
powder
initially
through
sol-gel
process
its
confirmation
proved
using
techniques
such
X-ray
diffraction
(XRD),
scanning
electron
microscopy
(SEM),
transmission
(TEM).
Then,
three
CMC/Z
scaffolds
were
made
contents:
group
A
(0
wt.%
Cup),
B
(2.5
Cup)
C
(5
Cup).
well-ordered
microporous
high
porosity
pore
connectivity,
observed
morphological
analysis
SEM.
Additionally,
size
more
homogeneous
than
that
groups
C.
There
no
significance
differences
in
physicochemical
characterization
among
groups.
Mechanical
properties
showed
values
compression
modulus
are
significantly
increased
addition
2.5%
into
CMCS/zein
matrix,
from
1.2
9.6
MPa.
incorporation
CMCS
along
zein
can
provide
suitable
substrate
for
growth
osteoblast
cells
after
implantation,
indicated
results
vitro
degradation.
cultured
MG-63
cells,
showing
cell
viability
content,
95%,
105%,
110%
pure
polymeric
scaffold,
reinforced
5%
Cup,
respectively.
As
result,
designed
possess
ability
be
used
tissue
engineering
due
having
characteristics
similar
natural
bone.
Language: Английский
Multifunctional nanofiber-based dressings in coordination with adipose-derived stem cells for accelerated burn wound healing
Materials & Design,
Journal Year:
2025,
Volume and Issue:
unknown, P. 113929 - 113929
Published: April 1, 2025
Language: Английский
Carboxymethyl chitosan-enhanced multi-level microstructured composite hydrogel scaffolds for bone defect repair
Yilong Wang,
No information about this author
Xingyu Zhou,
No information about this author
Junhui Jiang
No information about this author
et al.
Carbohydrate Polymers,
Journal Year:
2024,
Volume and Issue:
348, P. 122847 - 122847
Published: Oct. 14, 2024
Language: Английский
Icariin-loaded multilayered films deposited onto micro/nanostructured titanium enhances osteogenesis and reduces inflammation under diabetic conditions
Colloids and Interface Science Communications,
Journal Year:
2024,
Volume and Issue:
63, P. 100808 - 100808
Published: Oct. 29, 2024
Language: Английский