Organoid‐Like Neurovascular Spheroids Promote the Recovery of Hypoxic‐Ischemic Skin Flaps Through the Activation of Autophagy DOI Creative Commons
Junjin Jie,

Jingyi Ju,

Zhenxing Wang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Crosstalk between nerves and blood vessels plays a crucial role in flap development, injury repair, homeostasis maintenance. However, most transplantation strategies, the interactions have been ignored, leading to unsatisfactory repair effects. In this study, highly sprouting organoid‐like neurovascular spheroids (NVUs) with P34HB porous microsphere cores embedding supportive microenvironment of Gelatin Methacryloyl hydrogel are developed. Cell‐laden microspheres successfully recapitulated coupling by providing biomimetic extracellular for neural vascular cells at an vivo cell density. The results demonstrated that spheres formed complex plexuses secreted matrix, improving regeneration skin flap. Autophagy activation regulated is detected along assembly networks, suggesting its neovascularization. By incorporating fibroblasts, models composed dermis, vasculature, innervation facilely developed mimic dermal tissues. This stable reproducible vitro model can be utilized organ mechanistic exploration.

Language: Английский

Organoid‐Like Neurovascular Spheroids Promote the Recovery of Hypoxic‐Ischemic Skin Flaps Through the Activation of Autophagy DOI Creative Commons
Junjin Jie,

Jingyi Ju,

Zhenxing Wang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Crosstalk between nerves and blood vessels plays a crucial role in flap development, injury repair, homeostasis maintenance. However, most transplantation strategies, the interactions have been ignored, leading to unsatisfactory repair effects. In this study, highly sprouting organoid‐like neurovascular spheroids (NVUs) with P34HB porous microsphere cores embedding supportive microenvironment of Gelatin Methacryloyl hydrogel are developed. Cell‐laden microspheres successfully recapitulated coupling by providing biomimetic extracellular for neural vascular cells at an vivo cell density. The results demonstrated that spheres formed complex plexuses secreted matrix, improving regeneration skin flap. Autophagy activation regulated is detected along assembly networks, suggesting its neovascularization. By incorporating fibroblasts, models composed dermis, vasculature, innervation facilely developed mimic dermal tissues. This stable reproducible vitro model can be utilized organ mechanistic exploration.

Language: Английский

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