Zein/ZnO-Modified 3D-Printed PCL/Sphene Scaffolds with Improved Bacterial Inhibition and Osteoblast Activity for Bone Regeneration Applications DOI
Monireh Kouhi, Mohammad Khodaei, Bahareh Behrouznejad

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

3D printing offers a significant advantage in creating bioengineering scaffolds for patient-specific treatments of bony defects. In this study, 3D-printed polycaprolactone (PCL)/sphene (SP, CaTiSiO5) scaffold coated with zein/ZnO was fabricated to provide suitable environment bone regeneration. SP nanoparticles were synthesized using mechanochemical method and characterized by SEM-EDS, FTIR, XRD. 0-30 wt % prepared used fabricate PCL-based scaffolds. Incorporation into PCL (up 20 %) significantly increased compressive strength (from 37.5 65.2 MPa) modulus 0.33 0.63 MPa). vitro bioactivity evaluation simulated body fluid demonstrated the apatite formation ability PCL/SP scaffolds, as confirmed SEM-EDS analysis. Compared PCL/SP, zein/ZnO-modified showed surface hydrophilicity higher values bactericidal potency against S. aureus E. coli. Additionally, MTT assay, cell attachment, alkaline phosphatase activity revealed that zein ZnO coexistence on resulted proliferation, improved adhesion, enhanced osteogenic differentiation MG-63 cells compared unmodified samples. Overall, nanocomposite desirable physicochemical, mechanical, biological characteristics can serve superior platforms regeneration applications.

Language: Английский

Zein/ZnO-Modified 3D-Printed PCL/Sphene Scaffolds with Improved Bacterial Inhibition and Osteoblast Activity for Bone Regeneration Applications DOI
Monireh Kouhi, Mohammad Khodaei, Bahareh Behrouznejad

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

3D printing offers a significant advantage in creating bioengineering scaffolds for patient-specific treatments of bony defects. In this study, 3D-printed polycaprolactone (PCL)/sphene (SP, CaTiSiO5) scaffold coated with zein/ZnO was fabricated to provide suitable environment bone regeneration. SP nanoparticles were synthesized using mechanochemical method and characterized by SEM-EDS, FTIR, XRD. 0-30 wt % prepared used fabricate PCL-based scaffolds. Incorporation into PCL (up 20 %) significantly increased compressive strength (from 37.5 65.2 MPa) modulus 0.33 0.63 MPa). vitro bioactivity evaluation simulated body fluid demonstrated the apatite formation ability PCL/SP scaffolds, as confirmed SEM-EDS analysis. Compared PCL/SP, zein/ZnO-modified showed surface hydrophilicity higher values bactericidal potency against S. aureus E. coli. Additionally, MTT assay, cell attachment, alkaline phosphatase activity revealed that zein ZnO coexistence on resulted proliferation, improved adhesion, enhanced osteogenic differentiation MG-63 cells compared unmodified samples. Overall, nanocomposite desirable physicochemical, mechanical, biological characteristics can serve superior platforms regeneration applications.

Language: Английский

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