An overview on glycoside hydrolases and glycosyltransferases DOI
Maria Elena Ortiz‐Soto, Jürgen Seibel

Zeitschrift für Naturforschung C, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Language: Английский

Enzyme-enhanced acidogenic fermentation of waste activated sludge: Insights from sludge structure, interfaces, and functional microflora DOI
Ge Song, Shunan Zhao, Jiaqi Wang

et al.

Water Research, Journal Year: 2023, Volume and Issue: 249, P. 120889 - 120889

Published: Nov. 17, 2023

Language: Английский

Citations

20
Opportunities and challenges for plastic depolymerization by biomimetic catalysis DOI Creative Commons

Yanfen Wu,

Qikun Hu,

Yizhen Che

et al.

Chemical Science, Journal Year: 2024, Volume and Issue: 15(17), P. 6200 - 6217

Published: Jan. 1, 2024

Plastic waste has imposed significant burdens on the environment. Chemical recycling allows for repeated regeneration of plastics without deterioration in quality, but often requires harsh reaction conditions, thus being environmentally unfriendly. Enzymatic catalysis offers a promising solution under mild it faces inherent limitations such as poor stability, high cost, and narrow substrate applicability. Biomimetic may provide new avenue by combining enzyme-like activity with stability inorganic materials. demonstrated great potential biomass conversion recently shown progress plastic degradation. This perspective discusses biomimetic degradation from two perspectives: imitation active centers substrate-binding clefts. Given chemical similarity between plastics, relevant work is also included discussion to draw inspiration. We conclude this highlighting challenges opportunities achieving sustainable via approach.

Language: Английский

Citations

7
Structural insights into terminal arabinosylation of mycobacterial cell wall arabinan DOI Creative Commons

Yaqi Liu,

Chelsea M. Brown, Satchal K. Erramilli

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 29, 2025

Language: Английский

Citations

0
Hydrolytic nanozymes: Classification, activity regulation, and applications in sensing and degradation of environmental pollutants DOI
X.B. Liao, Xiao Wang, Bai Li

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 541, P. 216807 - 216807

Published: May 22, 2025

Language: Английский

Citations

0
Structural basis for inhibition of a GH116 β-glucosidase and its missense mutants by GBA2 inhibitors: Crystallographic and quantum chemical study DOI

Wijitra Meelua,

Natechanok Thinkumrob, Patchreenart Saparpakorn

et al.

Chemico-Biological Interactions, Journal Year: 2023, Volume and Issue: 384, P. 110717 - 110717

Published: Sept. 17, 2023

Language: Английский

Citations

7
Novel β-galactosidase activity and first crystal structure of Glycoside Hydrolase family 154 DOI Creative Commons
Lisanne Hameleers, Tjaard Pijning,

Brandon B. Gray

et al.

New Biotechnology, Journal Year: 2023, Volume and Issue: 80, P. 1 - 11

Published: Dec. 30, 2023

Polysaccharide Utilization Loci (PULs) are physically linked gene clusters conserved in the Gram-negative phylum of Bacteroidota and valuable sources for Carbohydrate Active enZyme (CAZyme) discovery. This study focuses on BD-β-Gal, an enzyme encoded a metagenomic PUL member Glycoside Hydrolase family 154 (GH154). BD-β-Gal showed exo-β-galactosidase activity with regiopreference hydrolyzing β-d-(1,6) glycosidic linkages. Notably, it exhibited preference d-glucopyranosyl (d-Glcp) over d-galactopyranosyl (d-Galp) d-fructofuranosyl (d-Fruf) at reducing end investigated disaccharides. In addition, we determined high resolution crystal structure thus providing first structural characterization GH154 enzyme. Surprisingly, this revealed (α/α)6 topology, which has not been observed before β-galactosidases. displayed low homology characterized CAZymes, but conservation analysis suggested that active site was located central cavity, E73, R252, D253 as putative catalytic residues. Interestingly, tetrameric flexible loop from neighboring protomer may contribute to its reaction specificity. Finally, founding GH154, BT3677 Bacteroides thetaiotaomicron, described β-glucuronidase, like lacked structure.

Language: Английский

Citations

4
Theoretical Insights into N-Glycoside Bond Cleavage of 5-Carboxycytosine by Thymine DNA Glycosylase: A QM/MM Study DOI

Wen-Juan Wang,

Tian Wang, Ying Zhao

et al.

The Journal of Physical Chemistry B, Journal Year: 2024, Volume and Issue: 128(19), P. 4621 - 4630

Published: May 2, 2024

Thymine DNA glycosylase (TDG)-mediated excision of 5-formylcytosine and 5-carboxylcytosine (5-caC) is a critical step in active demethylation. Herein, we employed combined quantum mechanics/molecular mechanics approach to investigate the reaction mechanism TDG-catalyzed N-glycosidic bond cleavage 5-caC. The calculated results show that 5-caC follows concerted (SN2) which glycosidic dissociation coupled with nucleophile attack. Protonation anion contributes N-glycoside bond, N3-protonated zwitterion imino tautomers are more favorable than carboxyl-protonated amino tautomers. This consistent experimental data. Furthermore, our reveal configuration rearrangement process protonated would lower stability substantially reduce barrier height for subsequent C1′–N1 cleavage. should be attributed smaller electrostatic repulsion between leaving base negative phosphate group as result structural rearrangement.

Language: Английский

Citations

1
Structural Characterization of β-xylosidase XynB2 from Geobacillus stearothermophilus CECT43: A Member of the Glycoside Hydrolase Family GH52 DOI Creative Commons
J.A. Gavira, Lellys M. Contreras,

Hassan Mohamad Alshamaa

et al.

Crystals, Journal Year: 2023, Volume and Issue: 14(1), P. 18 - 18

Published: Dec. 24, 2023

β-xylosidases (4-β-d-xylan xylohydrolase, E.C. 3.2.1.37) are glycoside hydrolases (GH) catalyzing the hydrolysis of (1→4)-β-d-xylans, allowing for removal β-d-xylose residues from its non-reducing termini. Together with other xylan-degrading enzymes, involved in enzymatic lignocellulosic biomass, making them highly valuable biotechnological field. Whereas different GH families deeply characterized a structural point view, GH52 family has been barely described. In this work, we report 2.25 Å resolution structure Geobacillus stearothermophilus CECT43 XynB2, providing second characterization family. A plausible dynamic loop closing entrance catalytic cleft is proposed based on comparison available structures, suggesting relevance dimeric members The glycone specificity at −1 site and GH116 also explained by our studies.

Language: Английский

Citations

1
Selective labelling of GBA2 in cells with fluorescent β-d-arabinofuranosyl cyclitol aziridines DOI Creative Commons
Qin Su, Max Louwerse, Rob Lammers

et al.

Chemical Science, Journal Year: 2024, Volume and Issue: 15(37), P. 15212 - 15220

Published: Jan. 1, 2024

Fluorescent β- d -arabinofuranosyl cyclitol aziridine activity-based probes potently and selectively label GBA2 both in vitro cellulo , allowing visualization of overexpressed using fluorescent microscopy.

Language: Английский

Citations

0
An overview on glycoside hydrolases and glycosyltransferases DOI
Maria Elena Ortiz‐Soto, Jürgen Seibel

Zeitschrift für Naturforschung C, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Language: Английский

Citations

0