Noncanonical Amino Acids: Bringing New-to-Nature Functionalities to Biocatalysis

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(19), P. 10877 - 10923

Published: Sept. 27, 2024

Biocatalysis has become an important component of modern organic chemistry, presenting efficient and environmentally friendly approach to synthetic transformations. Advances in molecular biology, computational modeling, protein engineering have unlocked the full potential enzymes various industrial applications. However, inherent limitations natural building blocks sparked a revolutionary shift.

Language: Английский

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Engineering of a (R)-selective Baeyer-Villiger monooxygenase to minimize overoxidation activity for asymmetric synthesis of active pharmaceutical prazoles

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 139561 - 139561

Published: Jan. 1, 2025

Language: Английский

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Codeine 3-O-demethylase catalyzed biotransformation of morphinan alkaloids in Escherichia coli: site directed mutagenesis of terminal residues improves enzyme expression, stability and biotransformation yield

Journal of Biological Engineering, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 19, 2025

The cultivation of opium poppy is the only commercially viable source most morphinan alkaloids. Bioproduction alkaloids in recombinant whole-cell systems provides a promising alternate these valuable compounds. enzyme codeine 3-O-demethylase can transform by O-demethylation and has been applied single step biotransformation reactions or as part larger biosynthetic cascade, however, productivity for remains low suboptimal properties could be improved. This mutagenesis study targeted non-conserved N-and C-terminal residues, which were replaced with equivalent residues from thebaine 6-O-demethylase. Whole cell performance was significantly improved Escherichia coli expressing mutants, ~ 2.8-fold increase production oripavine 1.3-fold morphine codeine. Statistical analysis yield, expression stability, predicted using changes Gibbs free energy (ΔΔG) deep-learning-based model DDmut, suggested that altered stability and/or soluble protein may contribute to observed improvements biotransformation. approach beneficial screening future mutations other enzymes.

Language: Английский

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Unleashing potential of microbial biosynthesis of monoterpenes via enzyme and metabolic engineering

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108525 - 108525

Published: Feb. 1, 2025

Language: Английский

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Digitalization of biocatalysis: Best practices to research data management

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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