Cytochrome P450-Catalyzed Allylic Oxidation of Pentalenene to 1-Deoxypentalenic Acid in Pentalenolactone Biosynthesis DOI Creative Commons
Jing Li, Chengde Zhang, Shiwen Wu

et al.

Engineering Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 100206 - 100206

Published: April 1, 2025

Language: Английский

The role and mechanisms of canonical and non-canonical tailoring enzymes in bacterial terpenoid biosynthesis DOI Creative Commons
Yuya Kakumu,

Ayesha Ahmed Chaudhri,

Eric J. N. Helfrich

et al.

Natural Product Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review explores recent insights into the roles and enzymatic mechanisms of canonical non-canonical tailoring enzymes in shaping modifying terpene hydrocarbon scaffolds during bacterial biosynthesis.

Language: Английский

Citations

0
Characterization of an α‐ketoglutarate‐dependent oxygenase involved in converting 2‐(2‐phenylethyl)chromones into 2‐styrylchromones in agarwood DOI Open Access
Mingliang Zhang,

Jiangping Fan,

Zekun Zhang

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(5)

Published: March 1, 2025

2-Phenylethylchromones (PECs) and 2-styrylchromones (SCs) are the primary components responsible for delightful fragrance bioactivity of agarwood, a highly valuable aromatic resinous heartwood. PECs derived from common precursor with diarylpentanoid skeleton (C6-C5-C6). However, biosynthesis SCs remains unclear. In this study, based on successful conversion PEC skeleton, rather than dehydrogenated diarylpentanoid, into by Aquilaria sinensis suspension cells, we demonstrated that double bond formation styryl group in occurs after creation not before step precursor. Through transcriptomic data mining, transient expression Nicotiana benthamiana A. identified new 2-oxoglutarate-dependent oxygenase (As2OG1) plays crucial role SCs. Further protein structure prediction mutagenesis studies, combined probing catalytic potential As2OG1 using chemically synthesized hydroxylated intermediates, suggested possibly uses diradical or carbocation to install bonds The results only provide insights molecular mechanism agarwood but also facilitate overproduction pharmaceutically important metabolic engineering approaches.

Language: Английский

Citations

0
Concise Biosynthesis of Antifungal Papulacandins DOI
Chao Yu,

Niandi Zhang,

Jinmei Li

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

We report the complete biosynthesis of antifungal papulacandins through heterologous expression and enzymatic assays. The papulacandin follows a convergent five-component strategy, involving linear polyketide chain that is synthesized installed to aryl-glucoside by synthase fused with C-terminal acyltransferase domain. formation challenging tricyclic benzannulated spiroketal core initiated C-glycosylation 5-(hydroxymethyl)resorcinol, followed spirocyclization catalyzed Fe(II)/α-ketoglutarate-dependent oxygenase PpcE.

Language: Английский

Citations

0
Cytochrome P450-Catalyzed Allylic Oxidation of Pentalenene to 1-Deoxypentalenic Acid in Pentalenolactone Biosynthesis DOI Creative Commons
Jing Li, Chengde Zhang, Shiwen Wu

et al.

Engineering Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 100206 - 100206

Published: April 1, 2025

Language: Английский

Citations

0