Cyclization diversity of meroditerpenoids from endophytic fungi of medicinal plants driven by synthetic biology strategies

Science of Traditional Chinese Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Abstract Background Medicinal plants rich in endophytic fungi are a significant source of natural lead compounds. Meroterpenoids, which hybrid products originating from partially terpenoid pathways, exhibit impressive structural complexity and substantial potential as drug candidates. The diversity meroterpenoids is largely attributed to the functional cyclases, generate variety compounds with different ring systems. This enzymatic versatility underscores biochemical their invaluable role discovery. Objective aim study was investigate Paris polyphylla var. yunnanensis facilitating diverse cyclization modifications meroditerpenoids through four terpene cyclases (TCs) Pyr4 family. Methods utilized recombinant strategy successfully reconstruct distinct TCs heterologous host, Aspergillus oryzae NSAR1. characterization resulting secondary metabolites performed using mass spectrometry NMR techniques. Results substitution endophytes felis 0260 Fusarium graminearum 1962 hydrophobic intermediates 1 2 , led production sartorypyrone C ( 3 ) new compound, 4′-methylchevalone E 4 ), respectively. demonstrates critical enhancing diversity. Conclusions These findings provide valuable insights into compatibility pathway combinations interchangeability derived medicinal plants, advanced understanding meroditerpenoid biosynthesis highlighted importance

Language: Английский

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Formation of N-Hydroxyethylisoindolinone Derivatives in Fungi Requires Highly Coordinated Consecutive Oxidation Steps

Organic Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Gene duplication significantly contributes to the diversification of biosynthetic potential and increases structural diversity secondary metabolites. Here, we report second alkyl salicylaldehyde derivative gene cluster in Penicillium roqueforti, being responsible for formation ethanolamine-containing derivatives. Heterologous expression feeding experiments provided evidence their via collaboration modification with one cytochrome P450 two flavin-containing monooxygenases a highly ordered manner before after ethanolamine incorporation.

Language: Английский

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DMSO enhances the biosynthesis of epoxyquinols in Pestalotiopsis sp. (strain IQ-011) and yields new [4 + 2] cycloaddition dimers

Organic & Biomolecular Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

DMSO-induced epigenetic modulation of Pestalotiopsis sp. IQ-011, and combined with metabolomic analysis, led to the discovery four novel metabolites, including three dimeric epoxyquinols generated via [4 + 2] cycloaddition.

Language: Английский

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Cupin-domain containing protein is not essential for the alkyl salicylaldehyde formation in Aspergillus ustus

Chemical Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Previous studies demonstrated the requirement of four enzymes including a cupin-domain containing protein for formation alkyl salicylaldehydes and derivatives. Heterologous expression three biosynthetic genes from

Language: Английский

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