Rational design of short‐chain dehydrogenase/reductase for enantio‐complementary synthesis of chiral 1,2‐diols by successive hydroxymethylation and reduction of aldehydes DOI

Xiu‐Xin Ren,

Bingmei Su, Xinqi Xu

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract Enantiopure 1,2‐diols are widely used in the production of pharmaceuticals, cosmetics, and functional materials as essential building blocks or bioactive compounds. Nevertheless, developing a mild, efficient environmentally friendly biocatalytic route for manufacturing enantiopure from simple substrate remains challenge. Here, we designed realized step‐wise cascade to access chiral starting aromatic aldehyde formaldehyde enabled by newly mined benzaldehyde lyase Sphingobium sp . combined with pair tailored‐made short‐chain dehydrogenase/reductase Pseudomonas monteilii (PmSDR‐MuR PmSDR‐MuS) capable producing ( R )‐ S )‐1‐phenylethane‐1,2‐diol 99% ee The planned could synthesize series broad scope (16 samples), excellent conversions (94%–99%), outstanding enantioselectivity (up ), making it an effective technique more sustainable manner.

Language: Английский

Innovative coupling pathway for second- and third-generation biomass: Efficient L-lactate synthesis from xylose and C1 compounds DOI

Ya Wu,

Gen Lu,

Ruiqi Zeng

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159573 - 159573

Published: Jan. 1, 2025

Language: Английский

Citations

0
Rational design of short‐chain dehydrogenase/reductase for enantio‐complementary synthesis of chiral 1,2‐diols by successive hydroxymethylation and reduction of aldehydes DOI

Xiu‐Xin Ren,

Bingmei Su, Xinqi Xu

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract Enantiopure 1,2‐diols are widely used in the production of pharmaceuticals, cosmetics, and functional materials as essential building blocks or bioactive compounds. Nevertheless, developing a mild, efficient environmentally friendly biocatalytic route for manufacturing enantiopure from simple substrate remains challenge. Here, we designed realized step‐wise cascade to access chiral starting aromatic aldehyde formaldehyde enabled by newly mined benzaldehyde lyase Sphingobium sp . combined with pair tailored‐made short‐chain dehydrogenase/reductase Pseudomonas monteilii (PmSDR‐MuR PmSDR‐MuS) capable producing ( R )‐ S )‐1‐phenylethane‐1,2‐diol 99% ee The planned could synthesize series broad scope (16 samples), excellent conversions (94%–99%), outstanding enantioselectivity (up ), making it an effective technique more sustainable manner.

Language: Английский

Citations

1