Exploring Nucleic Acid Nanozymes: A New Frontier in Biosensor Development

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 142 - 142

Published: Feb. 24, 2025

With the growing interest in nucleic acids and nanozymes, acid nanozymes (NANs) have emerged as a promising alternative to traditional enzyme catalysts, combining advantages of nanomaterials, are widely applied field biosensing. This review provides comprehensive overview recent studies on NAN-based biosensors. It classifies NANs based six distinct enzymatic activities: peroxidase-like, oxidase-like, catalase-like, superoxide dismutase-like, laccase-like, glucose oxidase-like. emphasizes how catalytic activity is significantly influenced by properties explores regulatory mechanisms governing NANs. Additionally, it systematically reviews important research progress colorimetric, fluorescent, electrochemical, SERS, chemiluminescent sensors, offering insights into development NAN biosensor applications.

Language: Английский

Spermine Enhances the Peroxidase Activities of Multimeric Antiparallel G-quadruplex DNAzymes

Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 12 - 12

Published: Jan. 2, 2025

G-quadruplex (G4) DNAzymes with peroxidase activities hold potential for applications in biosensing. While these nanozymes are easy to assemble, they not as efficient natural enzymes. Several approaches being used better understand the structural basis of their reaction mechanisms, a view designing constructs improved catalytic activities. Spermine alters structures and enhances some G4 DNAzymes. The reported effect spermine shifting conformation from antiparallel parallel has been tested on multimeric In this study, we examined effects multivalent Bcl2, c-MYC, PS2.M, PS5.M. Our findings show that significantly activity an DNAzyme, while there was no significant which already exists conformation. addition led substantial increase initial velocity PS2.M its form, enhancing it by approximately twofold. Therefore, enhancement offers promise expanding range available use biosensing tools.

Language: Английский

DNA nanosphere-enhanced DNAzyme system for sensitive and rapid detection of lead contamination in water and living cells

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 488, P. 137437 - 137437

Published: Jan. 28, 2025

Language: Английский