Exquisite Complex Reaction Cascade in the Natural 1,2,4-Triazine Assembly DOI

Yiyuan Cheng,

Haoran Pang, Wenjun Zhang

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 29, 2025

1,2,4-Triazine ring is a scaffold widely found in biologically active compounds, but how nature makes it remains enigmatic. In this study, we unveil the complex enzymatic and nonenzymatic cascade reactions that assemble 1,2,4-triazine moiety structures of natural products pseudoiodinine toxoflavin. Through biochemical studies, isotope labeling, application substrate analogues, propose plausible pathway for assembly from common precursor riboflavin biosynthesis. This process involves four two-electron oxidation steps, C-N bond formation, decarboxylation, N-N forming step catalyzed by metal-dependent WD40-repeat (WDR) protein. study thus not only provides first biocatalytic route also identifies previously unrecognized catalytic role large WDR protein family.

Language: Английский

Exquisite Complex Reaction Cascade in the Natural 1,2,4-Triazine Assembly DOI

Yiyuan Cheng,

Haoran Pang, Wenjun Zhang

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 29, 2025

1,2,4-Triazine ring is a scaffold widely found in biologically active compounds, but how nature makes it remains enigmatic. In this study, we unveil the complex enzymatic and nonenzymatic cascade reactions that assemble 1,2,4-triazine moiety structures of natural products pseudoiodinine toxoflavin. Through biochemical studies, isotope labeling, application substrate analogues, propose plausible pathway for assembly from common precursor riboflavin biosynthesis. This process involves four two-electron oxidation steps, C-N bond formation, decarboxylation, N-N forming step catalyzed by metal-dependent WD40-repeat (WDR) protein. study thus not only provides first biocatalytic route also identifies previously unrecognized catalytic role large WDR protein family.

Language: Английский

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