ACHIEVEMENTS IN PHYSICAL CHEMISTRY IN THE FIELD OF MICROSCOPY AND VISUALIZATION OF NANOSYSTEMS DOI Open Access
V. M. Оgenko

Ukrainian Chemistry Journal, Journal Year: 2023, Volume and Issue: 89(8), P. 63 - 77

Published: Sept. 29, 2023

The review presents modern views and the history of development microscopic studies nanosystems which heve been started 2014, after Nobel Prize in Chemistry was awarded to Eric Betzig, William Mörner, Stefan Gell "for super-resolved fluorescence microscopy". Their work ushered a new era optical microscopy, enabling precise examination individual molecules molecular clusters by using microscopes. By circumventing diffraction limitations that had constrained traditional microscopes, scientists gained access nanoscale realm, investigating structures within 1–100 nanometer range. Special attention is paid use carbon quantum dots plasmon resonance enhance when obtaining effect super-resolution images, allow microscopes estimation sizes cluster single molecules. This breakthrough removing li­mitation allowed working range nm obtain 3D images living cells. Particular achievements prospects high-resolution fluorescent nanoscopy SRM, successfully deve­lo­ping studying nanoworld at level scanning electron microscopy. In cell biology, nanomedicine, etc. are developing roadmaps for scientific breakthroughs visualization me­thods "live" images. Prospects Immuno-­SERS microscopy medicine indivi­dual diagnosis considered Key Findings: article highlights future SRM. High-resolution has proven instrumental advancing our understanding world range, akin capabilities microscopy. Within domains biology emerging, fueled super-re­so­lution imaging techniques, providing insights into cellular processes. horizons Immuno-SERS Microscopy Personalized Diagnostics Medicine expanding, promising exciting field medical diagnostics.

Language: Английский

Design strategies for tetrazine fluorogenic probes for bioorthogonal imaging DOI
Aiwen Yu, Xinyu He, Tianruo Shen

et al.

Chemical Society Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

In this review, recent progress in tetrazine-based fluorogenic probes for bioorthogonal chemistry is comprehensively analyzed, with a focus on advancements probe design and their imaging applications molecular processes live cells.

Language: Английский

Citations

1
Bioorthogonal Reactions in Bioimaging DOI Creative Commons
Eszter Kozma, Péter Kele

Topics in Current Chemistry, Journal Year: 2024, Volume and Issue: 382(1)

Published: Feb. 24, 2024

Abstract Visualization of biomolecules in their native environment or imaging-aided understanding more complex biomolecular processes are one the focus areas chemical biology research, which requires selective, often site-specific labeling targets. This challenging task is effectively addressed by bioorthogonal chemistry tools combination with advanced synthetic methods. Today, smart elements toolbox allows selective installation multiple markers to selected targets, enabling multicolor multimodal imaging biomolecules. Furthermore, recent developments bioorthogonally applicable probe design that meet growing demands superresolution microscopy enable questions be addressed. These novel, probes highly sensitive, low-background, single- multiphoton biological species and events live organisms at resolutions comparable size biomolecule interest. Herein, latest fluorescent schemes will discussed context cellulo/in vivo (multicolor and/or superresolved) schemes. The second part focuses on importance genetically engineered minimal tags, a particular interest protein tagging applications answer questions.

Language: Английский

Citations

7
Bioorthogonally activated probes for precise fluorescence imaging DOI Creative Commons
Youxin Fu, Xing Zhang, Luling Wu

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

This article provides a comprehensive overview of recent advances in the development bioorthogonally activated probes for labelling and precise situ fluorescence imaging both vitro vivo .

Language: Английский

Citations

7
Thioketal-photocage: a universal modification strategy for constructing new photochemical tools for real-time imaging in living cells DOI

Ding‐Heng Zhou,

Shanyong Chen, Yan-Zhao Liu

et al.

Science Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Recent advancements of fluorescent biosensors using semisynthetic probes DOI
Shahi Imam Reja, Masafumi Minoshima, Yuichiro Hori

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 247, P. 115862 - 115862

Published: Dec. 10, 2023

Language: Английский

Citations

8
MINFLUX nanoscopy: Visualising biological matter at the nanoscale level DOI Creative Commons
Alexander Carsten, Antonio Virgilio Failla, Martin Aepfelbacher

et al.

Journal of Microscopy, Journal Year: 2024, Volume and Issue: unknown

Published: April 25, 2024

Abstract Since its introduction in 2017, MINFLUX nanoscopy has shown that it can visualise fluorescent molecules with an exceptional localisation precision of a few nanometres. In this overview, we provide brief insight into technical implementations, marker developments and biological studies have been conducted connection imaging tracking. We also formulate ideas on how derived technologies could influence bioimaging the future. This is intended as general starting point for audience looking overview from theory to application.

Language: Английский

Citations

2
Photo-uncaging Triggers on Self-Blinking to Control Single-Molecule Fluorescence Kinetics for Super-resolution Imaging DOI
Ying Zheng, Zhiwei Ye, Xue Zhang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(28), P. 18477 - 18484

Published: June 28, 2024

Super-resolution imaging, especially a single-molecule localization approach, has raised fluorophore engineering revolution chasing sparse dark-bright blinking transforms. Yet, it is challenge to structurally devise fluorophores manipulating the kinetics. In this pursuit, we have developed triggering strategy by innovatively integrating photoactivatable nitroso-caging into self-blinking sulfonamide form nitroso-caged rhodamine (NOSR). Our demonstrated controllable events upon phototriggered caging unit release. This exceptional blink kinetics improved super-resolution imaging integrity on microtubules compared analogues. With aid of paramount fluorescence kinetics, successfully reconstructed ring structure nuclear pores and axial morphology mitochondrial outer membranes. We foresee that our synthetic approach photoactivation would facilitate devising for imaging.

Language: Английский

Citations

2
Chemo-Click: Receptor-Controlled and Bioorthogonal Chemokine Ligation for Real-Time Imaging of Drug-Resistant Leukemic B Cells DOI Creative Commons
Marco Bertolini, Lorena Mendive‐Tapia, Utsa Karmakar

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(44), P. 30565 - 30572

Published: Oct. 23, 2024

Drug resistance in B cell leukemia is characterized by the coexpression of CXCR5 and CXCR3 chemokine receptors, making it a valuable biomarker for patient stratification. Herein, we report novel platform activatable chemokines to selectively image drug-resistant leukemic cells first time. The C-terminal derivatization human CXCL13 CXCL10 with bioorthogonal tetrazine-BODIPY BCN groups retained binding internalization via their cognate receptors enabled rapid fluorescence labeling CXCR5+ CXCR3+ resistant cells─but not drug-susceptible cells─via intracellular ligation. This modular chemical approach offers versatile strategy real-time immunophenotyping populations distinct profiles will accelerate design new precision medicine tools advance personalized therapies blood tumors.

Language: Английский

Citations

2
Fluorogenic Tetrazine Bioorthogonal Probes for Advanced Application in Bioimaging and Biomedicine DOI Creative Commons
Wuyu Mao, Ping Dong, Wei Du

et al.

Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

A variety of bioorthogonal chemical tools have been developed and widely used in the study biological phenomena situ. Tetrazine chemistry exhibits ultrafast reaction kinetics, excellent biocompatibility, precise optical regulatory capabilities. Fluorogenic tetrazine probes achieved particularly diverse applications bioimaging disease diagnosis treatment. This Viewpoint briefly introduces characteristics advantages chemistry, some design strategies fluorogenic probes, status these to vivo imaging, as well Finally, we discuss challenges propose future trends field probes. offers insights into development new for biology research drugs.

Language: Английский

Citations

2
Photoactivatable aggregation‐induced emission luminogens based on photodehydrogenation reactions for biomedical applications DOI

Yuanyuan You,

Chengwei Tang,

Songling Lin

et al.

Luminescence, Journal Year: 2023, Volume and Issue: 39(2)

Published: Dec. 10, 2023

Abstract The development of photoactivatable aggregation‐induced emission (AIE) probes is one the hotspots for bioimaging and imaging‐guided precise disease therapy due to distinct advantages high spatiotemporal resolution, controllability, noninvasiveness light. To design develop novel AIE probes, functional groups based on photodehydrogenation reaction mechanisms are combined with AIE‐active skeleton. Here, recent progress in biomedical applications photocyclodehydrogenation photo‐oxidative dehydrogenation reactions summarized briefly. Moreover, outlook discussed aim at promoting innovative research applications.

Language: Английский

Citations

3