Rapid discovery and evolution of nanosensors containing fluorogenic amino acids DOI Creative Commons
Erkin Kuru, Jonathan Rittichier,

Helena de Puig

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 5, 2024

Language: Английский

“Zero” Intrinsic Fluorescence Sensing‐Platforms Enable Ultrasensitive Whole Blood Diagnosis and In Vivo Imaging DOI
Gangwei Jiang, Hong Liu,

Guohui Deng

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(16)

Published: Feb. 27, 2024

Abnormal physiological processes and diseases can lead to content or activity fluctuations of biocomponents in organelles whole blood. However, precise monitoring these abnormalities remains extremely challenging due the insufficient sensitivity accuracy available fluorescence probes, which be attributed background arising from two sources, 1) biocomponent autofluorescence (BCAF) 2) probe intrinsic (PIF). To overcome obstacles, we have re-engineered far-red NIR II rhodol derivatives that possess weak BCAF interference. And a series "zero" PIF sensing-platforms were created by systematically regulating open-loop/spirocyclic forms. Leveraging advancements, devised various ultra-sensitive indicators, achieving substantial boosts (190 1300-fold). Among 8-LAP demonstrated accurate tracking quantifying leucine aminopeptidase (LAP) blood at stages tumor metastasis. Furthermore, coupling with an endoplasmic reticulum-targeting element enabled detection ERAP1 HCT116 cells p53 abnormalities. This delicate design eliminating provides insights into enhancing existing probes toward imaging abnormal diseases.

Language: Английский

Citations

10
Recent advances in minimal fluorescent probes for optical imaging DOI Creative Commons

Fabio De Moliner,

Ferran Nadal‐Bufí, Marc Vendrell

et al.

Current Opinion in Chemical Biology, Journal Year: 2024, Volume and Issue: 80, P. 102458 - 102458

Published: April 25, 2024

Fluorescent probes have revolutionized biological imaging by enabling the real-time visualization of cellular processes under physiological conditions. However, their size and potential perturbative nature can pose challenges in retaining integrity functions. This manuscript highlights recent advancements development small fluorescent for optical studies. Single benzene-based fluorophores offer versatility with minimal disruption, exhibiting diverse properties like aggregation-induced emission pH responsiveness. nucleobases enable precise labeling nucleic acids without compromising function, offering high sensitivity compatibility biochemistry Bright yet amino provide an interesting alternative to bulky fusion proteins, facilitating non-invasive events precision. These miniaturized promise enhanced capabilities studying systems a manner, fostering further innovations molecular imaging.

Language: Английский

Citations

9
Recent trends in nanocellulose: Metabolism-related, gastrointestinal effects, and applications in probiotic delivery DOI
Ao Liu,

Hailian Wu,

Zishu Dong

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 343, P. 122442 - 122442

Published: July 1, 2024

Language: Английский

Citations

6
Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies DOI Creative Commons
Lei Zhang,

Dongwen Shen,

Jia-Zhen Yang

et al.

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
“Zero” Intrinsic Fluorescence Sensing‐Platforms Enable Ultrasensitive Whole Blood Diagnosis and In Vivo Imaging DOI
Gangwei Jiang, Hong Liu,

Guohui Deng

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(16)

Published: Feb. 27, 2024

Abstract Abnormal physiological processes and diseases can lead to content or activity fluctuations of biocomponents in organelles whole blood. However, precise monitoring these abnormalities remains extremely challenging due the insufficient sensitivity accuracy available fluorescence probes, which be attributed background arising from two sources, 1) biocomponent autofluorescence (BCAF) 2) probe intrinsic (PIF). To overcome obstacles, we have re‐engineered far‐red NIR II rhodol derivatives that possess weak BCAF interference. And a series “zero” PIF sensing‐platforms were created by systematically regulating open‐loop/spirocyclic forms. Leveraging advancements, devised various ultra‐sensitive indicators, achieving substantial boosts (190 1300‐fold). Among 8 ‐LAP demonstrated accurate tracking quantifying leucine aminopeptidase (LAP) blood at stages tumor metastasis. Furthermore, coupling with an endoplasmic reticulum‐targeting element enabled detection ERAP1 HCT116 cells p53 abnormalities. This delicate design eliminating provides insights into enhancing existing probes toward imaging abnormal diseases.

Language: Английский

Citations

2
Late-Stage Minimal Labeling of Peptides and Proteins for Real-Time Imaging of Cellular Trafficking DOI Creative Commons
Ferran Nadal‐Bufí, Raj V. Nithun,

Fabio De Moliner

et al.

ACS Central Science, Journal Year: 2024, Volume and Issue: 11(1), P. 66 - 75

Published: Nov. 26, 2024

The cellular uptake routes of peptides and proteins are complex diverse, often handicapping therapeutic success. Understanding their mechanisms internalization requires chemical derivatization with approaches that compatible wash-free real-time imaging. In this work, we developed a new late-stage labeling strategy for unprotected proteins, which retains biological activity while enabling live-cell imaging intracellular trafficking. Benzo-2,1,3-thiadiazoles were selectively incorporated into Cys residues both linear cyclic via Pd-mediated arylation good yields high purities. resulting labeled chemically stable under physiological conditions display strong fluorogenic character studies. We utilized approach to prepare native-like analogues cell-penetrating performed time-course analysis in live cells by fluorescence lifetime Furthermore, applied our label the chemokine protein mCCL2 monitor its receptor-mediated endocytosis macrophages. This study provides straightforward intact small direct visualization dynamic events.

Language: Английский

Citations

2
Introducing of an Unexplored Aza-BODIPY Diradicaloids with 4-(2,6-Ditert-butyl)phenoxyl Radicals Located in 1,7-Positions of the Aza-BODIPY Core DOI

Morris Oyelowo,

Jacob W. Schaffner,

Towhidi Illius Jeaydi

et al.

Inorganic Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

We have prepared and characterized two diradicaloid systems 5a 5b that originated from the oxidation of a 1,7-(4-(2,6-di-tert-butyl)phenol)-substituted aza-BODIPY core. The diradicaloids were by large array experimental computational methods. diamagnetic closed-shell state was postulated as ground in solution solid-state with substantial thermal population originating both open-shell diradical triplet states observed at room temperature. Transient absorption spectroscopy indicates fast (<10 ps) excited deactivation pathways associated target compounds' character Variable-temperature 1H NMR spectra indicate solvent dependency 5b. could be stepwise reduced to mixed-valence radical-anion dianion upon consequent single-electron reductions. Similarly, deprotonated aza-BODIPYs can oxidized form. Both dianionic forms exhibit an intense NIR region. Density functional theory (DFT) time-dependent DFT calculations used explain transformations UV–Vis-NIR all compounds.

Language: Английский

Citations

2
Rational Design of Pyrido[3,2-b]indolizine as a Tunable Fluorescent Scaffold for Fluorogenic Bioimaging DOI Creative Commons
Sihyeong Yi, Dahham Kim, Wansang Cho

et al.

JACS Au, Journal Year: 2024, Volume and Issue: 4(8), P. 2896 - 2906

Published: June 10, 2024

Novel fluorescent scaffolds are highly demanding for a wide range of applications in biomedical investigation. To meet this demand, the pyrido[3,2-

Language: Английский

Citations

1
Chemo-Click: Receptor-Controlled and Bioorthogonal Chemokine Ligation for Real-Time Imaging of Drug-Resistant Leukemic B Cells DOI Creative Commons
Marco Bertolini, Lorena Mendive‐Tapia, Utsa Karmakar

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(44), P. 30565 - 30572

Published: Oct. 23, 2024

Drug resistance in B cell leukemia is characterized by the coexpression of CXCR5 and CXCR3 chemokine receptors, making it a valuable biomarker for patient stratification. Herein, we report novel platform activatable chemokines to selectively image drug-resistant leukemic cells first time. The C-terminal derivatization human CXCL13 CXCL10 with bioorthogonal tetrazine-BODIPY BCN groups retained binding internalization via their cognate receptors enabled rapid fluorescence labeling CXCR5+ CXCR3+ resistant cells─but not drug-susceptible cells─via intracellular ligation. This modular chemical approach offers versatile strategy real-time immunophenotyping populations distinct profiles will accelerate design new precision medicine tools advance personalized therapies blood tumors.

Language: Английский

Citations

1
Near-Infrared-II-Activated Transition Metal(II)-Coordinated Ligand Radical Primes Robust Anticancer Immunity DOI

Qi Fan,

Yaming Wang, Hao Zhang

et al.

Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 67(23), P. 21329 - 21343

Published: Nov. 25, 2024

Photoactivatable metallodrugs combining tumor cell eradication and immune stimulation hold immense promise for targeted cancer therapy. However, limitations such as oxygen dependence, narrow visible light responsiveness, poor immunogenicity hinder their efficacy in deep solid tumors with hypoxic immunosuppressive microenvironments. Herein, we present a novel design strategy transition metal(II)-coordinated ligand radicals exhibiting intense near-infrared-II (NIR-II) absorption, unique endoplasmic reticulum-targeting capability, oxygen-independent photothermal performance, effectively addressing these constraints. Proof-of-concept results demonstrate the potent of our cobalt(II)-coordinated radical (

Language: Английский

Citations

1