Illuminating anions in biology with genetically encoded fluorescent biosensors DOI

Mariah Cook,

Shelby M. Phelps, Jasmine N. Tutol

et al.

Current Opinion in Chemical Biology, Journal Year: 2024, Volume and Issue: 84, P. 102548 - 102548

Published: Dec. 9, 2024

Language: Английский

Peroxidase-like Nanoparticles of Noble Metals Stimulate Increasing Sensitivity of Flavocytochrome b2-Based L-Lactate Biosensors DOI Creative Commons
Galina Gayda, Olha Demkiv, Nataliya Stasyuk

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(11), P. 562 - 562

Published: Nov. 20, 2024

We report the development of amperometric biosensors (ABSs) employing flavocytochrome

Language: Английский

Citations

1
A Genetically Encoded Fluorescent Biosensor for Intracellular Measurement of Malonyl-CoA DOI Creative Commons
Brodie L. Ranzau,

Tiffany D. Robinson,

Jack M. Scully

et al.

ACS Bio & Med Chem Au, Journal Year: 2024, Volume and Issue: 5(1), P. 184 - 193

Published: Dec. 18, 2024

Malonyl-CoA is the essential building block of fatty acids and regulates cell function through protein malonylation allosteric regulation signaling networks. Accordingly, production use malonyl-CoA finely tuned by cellular energy status. Most studies dynamics rely on bulk approaches that take only a snapshot average metabolic state population cells, missing out heterogeneous differences in acid biosynthesis could be occurring among population. To overcome this limitation, we have developed genetically encoded fluorescent protein-based biosensor for can used to capture single cells. This biosensor, termed Malibu (malonyl-CoA intracellular understand dynamics), exhibits an excitation-ratiometric change response binding. We first monitor during inhibition using cerulenin Escherichia coli, observing increase time- dose-dependent manner. In HeLa impact finding two inhibitors biosynthesis, orlistat, which inhibit different steps levels. Altogether, new malonyl-CoA, study providing unparalleled view into biosynthesis.

Language: Английский

Citations

1
A Genetically Encoded Fluorescent Biosensor for Intracellular Measurement of Malonyl-CoA DOI Creative Commons
Brodie L. Ranzau,

Tiffany D. Robinson,

Jack M Scully

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 28, 2024

Abstract Malonyl-CoA is the essential building block of fatty acids and regulates cell function through protein malonylation allosteric regulation signaling networks. Accordingly, production use malonyl-CoA finely tuned by cellular energy status. Most studies dynamics rely on bulk approaches that take only a snapshot average metabolic state population cells, missing out dynamic changes in acid biosynthesis could be occurring within single cell. To overcome this limitation, we have developed genetically encoded fluorescent protein-based biosensor for can used to capture cells. This biosensor, termed Malibu (malonyl-CoA i ntracellular understand dynamics), exhibits an excitation-ratiometric change response binding. We first monitor during inhibition using cerulenin E. coli , observing increase time- dose-dependent manner. In HeLa impact finding two inhibitors biosynthesis, orlistat, which inhibit different steps levels. Altogether, new malonyl-CoA, sensitively study providing unparalleled view into biosynthesis.

Language: Английский

Citations

0
LACCO Series: Genetically Encoded L-Lactate Biosensors DOI Open Access
Yusuke Nasu

Seibutsu Butsuri, Journal Year: 2024, Volume and Issue: 64(3), P. 155 - 158

Published: Jan. 1, 2024

何かとネガティブな印象がある「乳酸」.実はエネルギー源やシグナル分子として重要であることがわかってきた.この古くて新しい分子「乳酸」を⽣細胞や動物で観察可能な蛍光バイオセンサー"LACCOシリーズ"を開発した.本稿では,タンパク質工学によるLACCOシリーズの開発とその性能を概説する.

Language: Английский

Citations

0
A user-friendly fluorescent biosensor for precise lactate detection and quantification in vitro DOI
Qiwei Wang, Sai Shi, Si Liu

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

A stable, specific single fluorescent biosensor for in vitro lactate detection.

Language: Английский

Citations

0
Exploring Aptamer-aTF Sandwich and CRISPR-Cas12a Methods for Sensitive L-Lactate Biosensing in Human Serum and Saliva DOI
Mohammad A. Ansari,

Nurul Ajeerah Ali,

Muhammad Nadzre Adzremeen

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: unknown, P. 137015 - 137015

Published: Nov. 1, 2024

Language: Английский

Citations

0
Illuminating anions in biology with genetically encoded fluorescent biosensors DOI

Mariah Cook,

Shelby M. Phelps, Jasmine N. Tutol

et al.

Current Opinion in Chemical Biology, Journal Year: 2024, Volume and Issue: 84, P. 102548 - 102548

Published: Dec. 9, 2024

Language: Английский

Citations

0