Cyclooctatetraene-conjugated cyanine mitochondrial probes minimize phototoxicity in fluorescence and nanoscopic imaging DOI Creative Commons
Zhongtian Yang, Liuju Li, Jing Ling

et al.

Chemical Science, Journal Year: 2020, Volume and Issue: 11(32), P. 8506 - 8516

Published: Jan. 1, 2020

Cyclooctatetraene-conjugated cyanine dyes represent an effective strategy to improve biocompatibility under light in live-cell fluorescence imaging and analysis of mitochondria.

Language: Английский

Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP‐Tag Technologies DOI

Conner A. Hoelzel,

Xin Zhang

ChemBioChem, Journal Year: 2020, Volume and Issue: 21(14), P. 1935 - 1946

Published: March 17, 2020

Visualizing and manipulating the behavior of proteins is crucial to understanding physiology cell. Methods biorthogonal protein labeling are important tools attain this goal. In review, we discuss advances in probe technology specific for self-labeling tags, focusing mainly on application HaloTag SNAP-tag systems. We describe latest developments small-molecule probes that enable fluorogenic (no wash) imaging super-resolution fluorescence microscopy. addition, cover several methodologies perturbation or manipulation function towards control biological pathways. Thus, current technical systems means they becoming powerful visualization processes, providing invaluable scientific insights difficult obtain by traditional methodologies. As multiplex tag continues be developed expanded, utility these tags will allow researchers address previously inaccessible questions at forefront biology.

Language: Английский

Citations

107
Spatiotemporal coordination of transcription preinitiation complex assembly in live cells DOI Creative Commons
Vu Q. Nguyen, Anand Ranjan, Sheng Liu

et al.

Molecular Cell, Journal Year: 2021, Volume and Issue: 81(17), P. 3560 - 3575.e6

Published: Aug. 9, 2021

Language: Английский

Citations

99
Advanced imaging and labelling methods to decipher brain cell organization and function DOI
Daniel Choquet, Matthieu Sainlos, Jean‐Baptiste Sibarita

et al.

Nature reviews. Neuroscience, Journal Year: 2021, Volume and Issue: 22(4), P. 237 - 255

Published: March 12, 2021

Language: Английский

Citations

93
Illuminating RNA Biology: Tools for Imaging RNA in Live Mammalian Cells DOI Creative Commons
Esther Braselmann,

Colin M. Rathbun,

Erin M. Richards

et al.

Cell chemical biology, Journal Year: 2020, Volume and Issue: 27(8), P. 891 - 903

Published: July 7, 2020

Language: Английский

Citations

92
Cyclooctatetraene-conjugated cyanine mitochondrial probes minimize phototoxicity in fluorescence and nanoscopic imaging DOI Creative Commons
Zhongtian Yang, Liuju Li, Jing Ling

et al.

Chemical Science, Journal Year: 2020, Volume and Issue: 11(32), P. 8506 - 8516

Published: Jan. 1, 2020

Cyclooctatetraene-conjugated cyanine dyes represent an effective strategy to improve biocompatibility under light in live-cell fluorescence imaging and analysis of mitochondria.

Language: Английский

Citations

79