Expression, purification, and biochemical characterization of micro- and macroalgal kainoid synthases DOI

Austin R. Hopiavuori,

Radcliff T Huffman,

Shaun M. K. McKinnie

et al.

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2024, Volume and Issue: unknown, P. 233 - 258

Published: Jan. 1, 2024

Language: Английский

Computational Study Into the Oxidative Ring‐Closure Mechanism During the Biosynthesis of Deoxypodophyllotoxin DOI Creative Commons

Fintan G. Hardy,

Henrik P. H. Wong, Sam P. de Visser

et al.

Chemistry - A European Journal, Journal Year: 2024, Volume and Issue: 30(22)

Published: Feb. 7, 2024

The nonheme iron dioxygenase deoxypodophyllotoxin synthase performs an oxidative ring-closure reaction as part of natural product synthesis in plants. How the enzyme enables (-)-yatein and avoids substrate hydroxylation remains unknown. To gain insight into mechanism understand details pathways leading to products by-products we performed a comprehensive computational study. work shows that is bound tightly binding pocket with C

Language: Английский

Citations

8
Discovery of Noncanonical Iron and 2-Oxoglutarate Dependent Enzymes Involved in C–C and C–N Bond Formation in Biosynthetic Pathways DOI Creative Commons
Yaoyao Shen, Aimin Sun, Yisong Guo

et al.

ACS Bio & Med Chem Au, Journal Year: 2025, Volume and Issue: 5(2), P. 238 - 261

Published: March 10, 2025

Iron and 2-oxoglutarate dependent (Fe/2OG) enzymes utilize an FeIV═O species to catalyze the functionalization of otherwise chemically inert C–H bonds. In addition more familiar canonical reactions hydroxylation chlorination, they also several other types that contribute diversity complexity natural products. past decade, new Fe/2OG C–C C–N bond formation have been reported in biosynthesis structurally complex Compared with catalytic cycles these involve distinct mechanistic features enable noncanonical reaction outcomes. This Review summarizes recent discoveries involved a focus on mechanisms their roles product biosynthesis.

Language: Английский

Citations

0
Unraveling the Mechanism of the Oxidative C–C Bond Coupling Reaction Catalyzed by Deoxypodophyllotoxin Synthase DOI

Yanzhuang Su,

Wenzhen Lai

Inorganic Chemistry, Journal Year: 2024, Volume and Issue: 63(30), P. 13948 - 13958

Published: July 15, 2024

Deoxypodophyllotoxin synthase (DPS), a nonheme Fe(II)/2-oxoglutarate (2OG)-dependent oxygenase, is key enzyme that involved in the construction of fused-ring system (-)-podophyllotoxin biosynthesis by catalyzing C-C coupling reaction. However, mechanistic details DPS-catalyzed ring formation remain unclear. Herein, our quantum mechanics/molecular mechanics (QM/MM) calculations reveal novel mechanism involves recycling CO

Language: Английский

Citations

2
Expression, purification, and biochemical characterization of micro- and macroalgal kainoid synthases DOI

Austin R. Hopiavuori,

Radcliff T Huffman,

Shaun M. K. McKinnie

et al.

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2024, Volume and Issue: unknown, P. 233 - 258

Published: Jan. 1, 2024

Language: Английский

Citations

1