Analytical and Bioanalytical Chemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 23, 2024
Language: Английский
Clinical Proteomics, Journal Year: 2024, Volume and Issue: 21(1)
Published: Feb. 22, 2024
Abstract Serum or plasma is frequently utilized in biomedical research; however, its application impeded by the requirement for invasive sample collection. The non-invasive nature of urine collection makes it an attractive alternative disease characterization and biomarker discovery. Mass spectrometry-based protein profiling has led to discovery several disease-associated biomarkers. Proteomic analysis not only been applied disorders kidney urinary bladder but also conditions affecting distant organs because proteins excreted originate from multiple organs. This review provides a progress update on proteomics carried out over past decade. Studies summarized this have expanded catalog detected variety clinical conditions. wide range applications analysis—from characterizing diseases discovering predictive, diagnostic prognostic markers—continues drive investigations proteome.
Language: Английский
Citations
20
Journal of Chromatography B, Journal Year: 2025, Volume and Issue: 1252, P. 124461 - 124461
Published: Jan. 10, 2025
Language: Английский
Citations
1
PROTEOMICS, Journal Year: 2024, Volume and Issue: 24(12-13)
Published: Jan. 3, 2024
Abstract Glycosylation, the major post‐translational modification of proteins, significantly increases diversity proteoforms. Glycans are involved in a variety pivotal structural and functional roles changes glycosylation profoundly connected to progression numerous diseases. Mass spectrometry (MS) has emerged as gold standard for glycan glycopeptide analysis because its high sensitivity wealth fragmentation information that can be obtained. Various separation techniques have been employed resolve isomers at front end MS. However, differentiating structures isobaric isomeric glycopeptides constitutes challenge MS‐based characterization. Many reports described use various ion mobility–mass (IM–MS) glycomic analyses. Nevertheless, very few studies focused on N ‐ O ‐linked site‐specific glycopeptidomic analysis. Unlike glycomics, glycoproteomics presents multitude inherent challenges microheterogeneity, which further exacerbated by lack dedicated bioinformatics tools. In this review, we cover recent advances made towards growing field using IM–MS with specific emphasis MS capabilities resolving peptidoglycan structures. Furthermore, discuss commonly used software supports data glycopeptides.
Language: Английский
Citations
5
Acta Biochimica et Biophysica Sinica, Journal Year: 2024, Volume and Issue: 56(8), P. 1208 - 1220
Published: Aug. 1, 2024
Aging, a complex biological process, involves the progressive decline of physiological functions across various systems, leading to increased susceptibility neurodegenerative diseases. In society, demographic aging imposes significant economic and social burdens due these conditions. This review specifically examines association protein glycosylation with Glycosylation, critical post-translational modification, influences numerous aspects function that are pivotal in pathophysiology diseases such as Alzheimer's disease, Parkinson's other We highlight alterations patterns observed during aging, their implications onset progression diseases, potential profiles biomarkers for early detection, prognosis, monitoring age-associated conditions, delve into mechanisms glycosylation. Furthermore, this explores role regulating mediating interactions By examining changes associated each part, underscores research tool enhance our understanding its related
Language: Английский
Citations
4
Analytical and Bioanalytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 13, 2025
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: April 5, 2025
Postoperative recurrence of pancreatic adenocarcinoma (PAAD) remains a major challenge. This study aims to establish and validate lipid metabolism-related prognostic model predict in PAAD patients. The TCGA-PAAD database was used training cohort, which validated using the ICGC multiple center cohorts. A based on LASSO Cox regression nomogram developed further validated. Among 196 genes, four were selected for model. Patients stratified into high- low-risk groups risk score. Univariate multivariate analyses showed that tumor site, T stage, N M score significantly associated with progression-free interval (PFI). High-risk patients had worse PFI, overall survival (OS), disease-specific (DSS) (all P < 0.05). Time-dependent ROC decision curve confirmed superior diagnostic capacity nomogram. GSEA revealed enrichment G2M checkpoint, glycolysis, estrogen response, hypoxia pathways high-risk group. Additionally, scores correlated poor immune infiltration, gene mutations, mutational burden (TMB). Single-cell analysis suggested genes interact various cell types promote progression. novel patients, strong accuracy stability.
Language: Английский
Citations
0
Acta Biochimica et Biophysica Sinica, Journal Year: 2024, Volume and Issue: unknown
Published: June 1, 2024
N-glycans play important roles in a variety of biological processes. In recent years, analytical technologies with high resolution and sensitivity have advanced exponentially, enabling analysts to investigate N-glycomic changes different states. Specific glycan glycosylation signatures been identified multiple diseases, including cancer, autoimmune nervous system disorders, metabolic cardiovascular diseases. These glycans demonstrate comparable or superior indicating capability disease diagnosis prognosis over routine biomarkers. Moreover, synchronous alterations concurrent initiation progression provide novel insights into pathogenetic mechanisms potential treatment targets. This review elucidates the significance N-glycans, compares existing glycomic technologies, delineates clinical performance across range
Language: Английский
Citations
2
Expert Review of Proteomics, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 22, 2024
The identification and characterization of glycopeptides through LC-MS/MS advanced enrichment techniques are crucial for advancing clinical glycoproteomics, significantly impacting the discovery disease biomarkers therapeutic targets. Despite progress in methods like Lectin Affinity Chromatography (LAC), Hydrophilic Interaction Liquid (HILIC), Electrostatic Repulsion (ERLIC), issues with specificity, efficiency, scalability remain, impeding thorough analysis complex glycosylation patterns understanding.
Language: Английский
Citations
1
Glycobiology, Journal Year: 2024, Volume and Issue: 34(9)
Published: July 26, 2024
Abstract N-linked glycoproteins are rich in seminal plasma, playing essential roles supporting sperm function and fertilization process. The alteration of plasma glycans its correspond may lead to dysfunction even infertility. In present study, an integrative analysis glycoproteomic proteomic was performed investigate the changes site-specific glycoptoteins asthenozoospermia. By large scale profiling quantifying 5,018 intact N-glycopeptides we identified 92 from 34 changed Especially, fucosylated containing lewis x, y core fucosylation were significantly up-regulated asthenozoospermia compared healthy donors. up-regulation interfere surface compositions regulation immune response, which subsequently disrupts function. Three differentiated expression vesicle-specific (fibronectin, seminogelin-2, glycodelin) also detected with interpretation altered glycan structures provides data for diagnosis etiology male infertility, as well providing new insights into potential therapeutic targets
Language: Английский
Citations
0
Molecules, Journal Year: 2024, Volume and Issue: 29(22), P. 5241 - 5241
Published: Nov. 6, 2024
Extracellular vesicles (EVs) are a population of nanoscale particles surrounded by phospholipid bilayer, enabling intercellular transfer bioactive molecules. Once released from the parental cell, EVs can be found in most biological fluids human body and isolated them. For this reason, have significant diagnostic potential serve as an excellent source circulating disease biomarkers. Protein glycosylation plays key role many processes, aberrant is hallmark various diseases. been shown to carry multiple glycoproteins, but little known about specific roles these glycoproteins context EVs. Moreover, changes EV described for several diseases, including cancers metabolic, cardiovascular, neurological or kidney Urine richest EVs, providing almost unlimited (in terms volume) opportunities non-invasive isolation. Recent studies also revealed pathological link between urinary urological cancers, well other pathologies tract. In review, we discuss recent research advances field diagnostic/prognostic glycosylation. addition, summarize common methods isolating urine techniques used study their
Language: Английский
Citations
0