Evaluation of the feasibility of reusing polyurethane foam (PUF) cartridges for monitoring bioaerosols DOI Open Access
Guillermo M. Horta-Valerdi,

Paula Montserrat Crespo-Barrera,

Amado Enrique Navarro-Frómeta

et al.

MOJ Ecology & Environmental Sciences, Journal Year: 2024, Volume and Issue: 9(4), P. 186 - 188

Published: Aug. 26, 2024

Bioaerosols are a fundamental component of particulate matter present in the air, and numerous sampling detection techniques have been developed to study them. While simultaneous determination organics microorganisms could offer advantages, this approach has not well explored. Some for analyzing organic compounds involving using polyurethane foam (PUF) cartridge or disk which, depending on analytic procedure, may need be cleaned with chemicals that compromise growth recovery if used purpose. This aims determine whether chemical pretreatment PUF affect negatively results sampling. Additionally, potential microorganism previously was evaluated. After sterilization, demonstrated is possible both chemically treated reused PUF. These findings suggest reusing reduce costs waste after

Language: Английский

Out in the Open: Investigating Passive Airborne eDNA Detection of Bats at Artificial Feeding Stations DOI Creative Commons
Daniel E. Sanchez, Faith M. Walker,

Savannah J. Marriott

et al.

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(3)

Published: May 1, 2025

ABSTRACT Environmental DNA (eDNA) is a valuable biomonitoring tool, but application in terrestrial settings remains challenging due to lack of generalizable sampling approaches. With bat species needing urgent research attention, airborne eDNA may offer this generalizability, as current for bats mostly limited conspicuous sources (e.g., guano). While previous studies detected from roosts and open‐air sites using active air sampling, it uncertain whether can be readily the open passive In central Texas, we used determine if could recover assemblages with metabarcoding an imperiled focal (tricolored bat, Perimyotis subflavus ) qPCR. Outside two cave locations, positioned samplers (two collection media per sampler; n = 24 media) near artificial prey patches, monitoring acoustically activity foraging. lab, subjected multiple extraction methods, direct extraction, resuspension‐concentration approaches (filtration pelleting). Metabarcoding allowed detection within single sample, while qPCR P. samples. Although detections all came pelleting substantially improved taxonomic recovery sample success vertebrates overall. Detection establishes lower bound possibility settings, low number highlights need strategies. We recommendations enhance future efforts introduce assay that variety contexts.

Language: Английский

Citations

0

Cross-cutting research and future directions under the GAPS networks DOI Creative Commons
Tom Harner, Amandeep Saini, Pourya Shahpoury

et al.

Environmental Science Advances, Journal Year: 2024, Volume and Issue: 3(6), P. 798 - 807

Published: Jan. 1, 2024

The GAPs networks are engaging in cross-cutting studies across fields of science and policy to address future challenges.

Language: Английский

Citations

0

Evaluation of the feasibility of reusing polyurethane foam (PUF) cartridges for monitoring bioaerosols DOI Open Access
Guillermo M. Horta-Valerdi,

Paula Montserrat Crespo-Barrera,

Amado Enrique Navarro-Frómeta

et al.

MOJ Ecology & Environmental Sciences, Journal Year: 2024, Volume and Issue: 9(4), P. 186 - 188

Published: Aug. 26, 2024

Bioaerosols are a fundamental component of particulate matter present in the air, and numerous sampling detection techniques have been developed to study them. While simultaneous determination organics microorganisms could offer advantages, this approach has not well explored. Some for analyzing organic compounds involving using polyurethane foam (PUF) cartridge or disk which, depending on analytic procedure, may need be cleaned with chemicals that compromise growth recovery if used purpose. This aims determine whether chemical pretreatment PUF affect negatively results sampling. Additionally, potential microorganism previously was evaluated. After sterilization, demonstrated is possible both chemically treated reused PUF. These findings suggest reusing reduce costs waste after

Language: Английский

Citations

0