Out in the Open: Investigating Passive Airborne eDNA Detection of Bats at Artificial Feeding Stations
Environmental DNA,
Journal Year:
2025,
Volume and Issue:
7(3)
Published: May 1, 2025
ABSTRACT
Environmental
DNA
(eDNA)
is
a
valuable
biomonitoring
tool,
but
application
in
terrestrial
settings
remains
challenging
due
to
lack
of
generalizable
sampling
approaches.
With
bat
species
needing
urgent
research
attention,
airborne
eDNA
may
offer
this
generalizability,
as
current
for
bats
mostly
limited
conspicuous
sources
(e.g.,
guano).
While
previous
studies
detected
from
roosts
and
open‐air
sites
using
active
air
sampling,
it
uncertain
whether
can
be
readily
the
open
passive
In
central
Texas,
we
used
determine
if
could
recover
assemblages
with
metabarcoding
an
imperiled
focal
(tricolored
bat,
Perimyotis
subflavus
)
qPCR.
Outside
two
cave
locations,
positioned
samplers
(two
collection
media
per
sampler;
n
=
24
media)
near
artificial
prey
patches,
monitoring
acoustically
activity
foraging.
lab,
subjected
multiple
extraction
methods,
direct
extraction,
resuspension‐concentration
approaches
(filtration
pelleting).
Metabarcoding
allowed
detection
within
single
sample,
while
qPCR
P.
samples.
Although
detections
all
came
pelleting
substantially
improved
taxonomic
recovery
sample
success
vertebrates
overall.
Detection
establishes
lower
bound
possibility
settings,
low
number
highlights
need
strategies.
We
recommendations
enhance
future
efforts
introduce
assay
that
variety
contexts.
Language: Английский
Cross-cutting research and future directions under the GAPS networks
Environmental Science Advances,
Journal Year:
2024,
Volume and Issue:
3(6), P. 798 - 807
Published: Jan. 1, 2024
The
GAPs
networks
are
engaging
in
cross-cutting
studies
across
fields
of
science
and
policy
to
address
future
challenges.
Language: Английский
Evaluation of the feasibility of reusing polyurethane foam (PUF) cartridges for monitoring bioaerosols
MOJ Ecology & Environmental Sciences,
Journal Year:
2024,
Volume and Issue:
9(4), P. 186 - 188
Published: Aug. 26, 2024
Bioaerosols
are
a
fundamental
component
of
particulate
matter
present
in
the
air,
and
numerous
sampling
detection
techniques
have
been
developed
to
study
them.
While
simultaneous
determination
organics
microorganisms
could
offer
advantages,
this
approach
has
not
well
explored.
Some
for
analyzing
organic
compounds
involving
using
polyurethane
foam
(PUF)
cartridge
or
disk
which,
depending
on
analytic
procedure,
may
need
be
cleaned
with
chemicals
that
compromise
growth
recovery
if
used
purpose.
This
aims
determine
whether
chemical
pretreatment
PUF
affect
negatively
results
sampling.
Additionally,
potential
microorganism
previously
was
evaluated.
After
sterilization,
demonstrated
is
possible
both
chemically
treated
reused
PUF.
These
findings
suggest
reusing
reduce
costs
waste
after
Language: Английский