Microalgae
are
photosynthetic
microorganisms
which
can
grow
under
a
large
spectrum
of
conditions
and
may
produce
number
valuable
products.
However,
increased
chlorophyll
concentration
constitutes
limiting
factor,
especially
in
large-scale
photo-bioreactors,
causing
“shadow
effect”
negatively
influences
biomass
productivity.
The
main
scope
the
present
study
was
to
select
low
content
strain
Stichococcus
sp.,
order
increase
light
penetration
into
culture
photo-bioreactors.
Selection
through
random
mutation
using
ethyl
methanesulfonate
(EMS).
Two
promising
mutants
(EMS1
&
EMS2)
were
isolated
cultivated
for
three
generations
on
solid
medium
(petri
dishes).
selected
strains
then
open
beaker
glassware
with
200
mL
F/2
medium,
diluted
natural
seawater,
sterile
conditions.
EMS1,
best
performing
mutant
strain,
exhibited
lower
(43%),
higher
(12%),
lipids
(45%),
carbohydrates
(28%)
production,
compared
wild
type.
EMS1
proved
contain
least
(6.29
±
0.52
mg
g-1),
yet
highest
(2.47
0.10
g
L-1),
(21%
dry
biomass),
(44%
biomass)
type
strain.
Protein
did
not
differ
significantly
among
strains.
sp.
has
high
potential
further
investigation
production
various
bio-chemicals.
Water,
Journal Year:
2023,
Volume and Issue:
15(14), P. 2605 - 2605
Published: July 18, 2023
Marimo
is
a
type
of
microalgal-bacterial
granular
sludge
(MBGS)
that
exists
in
natural
water
bodies.
For
the
first
time,
this
paper
explored
feasibility
marimo
real
wastewater
effluent
polishing,
focusing
on
nutrient
removal
as
compared
with
MBGS.
The
results
showed
color
gradually
darkened
during
21-day
experiment,
and
chlorophyll
content
increased
significantly.
Although
MBGS
fairly
similar
performance
terms
NO3−-N
TN,
exhibited
better
phosphate
to
contained
different
algae
but
same
bacterial
phylum
Proteobacteria,
including
denitrifiers.
In
addition,
had
higher
relative
abundance
nitrite
reductase
than
MBGS,
suggesting
denitrification
process
might
also
happen
addition
assimilation.
This
study
expected
initiate
application
for
polishing
reclamation,
shedding
light
nature-based
self-purification
technology
era
carbon
neutrality.
