Metrics to relate COVID-19 wastewater data to clinical testing dynamics

Water Research, Journal Year: 2022, Volume and Issue: 212, P. 118070 - 118070

Published: Jan. 14, 2022

Wastewater surveillance has emerged as a useful tool in the public health response to COVID-19 pandemic. While wastewater been applied at various scales monitor population-level dynamics, there is need for quantitative metrics interpret data context of trends. 24-hour composite samples were collected from March 2020 through May 2021 Massachusetts treatment plant and SARS-CoV-2 RNA concentrations measured using RT-qPCR. The relationship between copy numbers gene fragments clinical cases deaths varies over time. We demonstrate utility three new changes epidemiology: (1) ratio (WC ratio), (2) time lag reporting, (3) transfer function case curves. WC increases after key events, providing insight into balance disease spread response. Time analysis showed that preceded clinically reported first wave pandemic but did not serve leading indicator second wave, likely due increased testing capacity, which allows more timely detection reporting. These could help further integrate future pandemics.

Language: Английский

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Does normalization of SARS-CoV-2 concentrations by Pepper Mild Mottle Virus improve correlations and lead time between wastewater surveillance and clinical data in Alberta (Canada): comparing twelve SARS-CoV-2 normalization approaches

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 856, P. 158964 - 158964

Published: Sept. 24, 2022

Language: Английский

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Wastewater-based prediction of COVID-19 cases using a highly sensitive SARS-CoV-2 RNA detection method combined with mathematical modeling

Environment International, Journal Year: 2023, Volume and Issue: 173, P. 107743 - 107743

Published: Jan. 7, 2023

Wastewater-based epidemiology (WBE) has the potential to predict COVID-19 cases; however, reliable methods for tracking SARS-CoV-2 RNA concentrations (C

Language: Английский

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Impact of the COVID-19 pandemic on the prevalence of influenza A and respiratory syncytial viruses elucidated by wastewater-based epidemiology

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 880, P. 162694 - 162694

Published: March 8, 2023

Since the COVID-19 pandemic, a decrease in prevalence of Influenza A virus (IAV) and respiratory syncytial (RSV) has been suggested by clinical surveillance. However, there may be potential biases obtaining an accurate overview infectious diseases community. To elucidate impact on IAV RSV, we quantified RSV RNA wastewater collected from three treatment plants (WWTPs) Sapporo, Japan, between October 2018 January 2023, using highly sensitive EPISENS™ method. From to April 2020, M gene concentrations were positively correlated with confirmed cases corresponding area (Spearman's r = 0.61). Subtype-specific HA genes also detected, their showed trends that consistent clinically reported cases. B serotypes detected wastewater, 0.36-0.52). The detection ratios decreased 66.7 % (22/33) 42.4 (14/33) 4.56 (12/263) 32.7 (86/263), respectively city after prevalence. present study demonstrates usefulness wastewater-based epidemiology combined preservation (wastewater banking) as tool for better management viral diseases.

Language: Английский

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Wastewater-based epidemiology predicts COVID-19-induced weekly new hospital admissions in over 150 USA counties

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 28, 2023

Although the coronavirus disease (COVID-19) emergency status is easing, COVID-19 pandemic continues to affect healthcare systems globally. It crucial have a reliable and population-wide prediction tool for estimating COVID-19-induced hospital admissions. We evaluated feasibility of using wastewater-based epidemiology (WBE) predict weekly new hospitalizations in 159 counties across 45 states United States America (USA), covering population nearly 100 million. Using county-level wastewater surveillance data (over 20 months), WBE-based models were established through random forest algorithm. accurately predicted admissions, allowing preparation window 1-4 weeks. In real applications, periodically updated showed good accuracy transferability, with mean absolute error within 4-6 patients/100k upcoming hospitalization numbers. Our study demonstrated potential WBE as an effective method provide early warnings systems.

Language: Английский

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Monitoring human arboviral diseases through wastewater surveillance: Challenges, progress and future opportunities

Water Research, Journal Year: 2022, Volume and Issue: 223, P. 118904 - 118904

Published: July 25, 2022

Language: Английский

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Wastewater surveillance of SARS-CoV-2 and chemical markers in campus dormitories in an evolving COVID − 19 pandemic

Journal of Hazardous Materials, Journal Year: 2022, Volume and Issue: 446, P. 130690 - 130690

Published: Dec. 28, 2022

In this study, we report the implementation of a comprehensive wastewater surveillance testing program at university campus in Singapore to identify Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infected individuals and usage pharmaceuticals personal care products (PPCPs) as well other emerging contaminants (ECs). This unique co-monitoring simultaneously measured SARS-CoV-2 with chemical markers/contaminants COVID-19 situation evolved from pandemic endemic stages, following nationwide mass vaccination drive. RNA concentrations dormitories were using real-time reverse transcription-polymerase chain reaction (RT-qPCR) corroborated number symptomatic cases confirmed antigen rapid test (ART). Consistent results observed where detected increased proportionately residing on campus. Similarly, wide range ECs, including disinfectants antibiotics, through sensitive liquid chromatography tandem spectrometry (LC-MS/MS) techniques establish PPCPs consumption patterns during various stages Singapore. Statistical correlation was few ECs belonging disinfectants, PCPs antibiotics. A high concentration subsequent positive reported indicates that could serve marker such unprecedented times.

Language: Английский

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A multistate assessment of population normalization factors for wastewater-based epidemiology of COVID-19

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(4), P. e0284370 - e0284370

Published: April 12, 2023

Wastewater-based epidemiology (WBE) has become a valuable tool for monitoring SARS-CoV-2 infection trends throughout the COVID-19 pandemic. Population biomarkers that measure relative human fecal contribution to normalize wastewater concentrations are needed improved analysis and interpretation of community trends. The Centers Disease Control Prevention National Wastewater Surveillance System (CDC NWSS) recommends using flow rate or indicators as population normalization factors. However, there is no consensus on which factor performs best. In this study, we provided first multistate assessment effects (crAssphage, F+ Coliphage, PMMoV) correlation cases CDC NWSS dataset 182 communities across six U.S. states. Flow normalized produced strongest with cases. from three were significantly lower than rate. Additionally, reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) values over samples analyzed real-time quantitative (rRT-qPCR). Our shows utilizing RT-ddPCR generate between

Language: Английский

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The Present and Future Landscapes of Molecular Diagnostics

Annual Review of Analytical Chemistry, Journal Year: 2024, Volume and Issue: 17(1), P. 459 - 474

Published: Feb. 16, 2024

Nucleic acid testing is the cornerstone of modern molecular diagnostics. This review describes current status and future directions diagnostics, focusing on four major techniques: polymerase chain reaction (PCR), next-generation sequencing (NGS), isothermal amplification methods such as recombinase (RPA) loop-mediated (LAMP), clustered regularly interspaced short palindromic repeats (CRISPR)-based detection methods. We explore advantages limitations each technique, describe how overlaps with or complements other techniques, examine clinical offerings. provides a broad perspective into landscape diagnostics highlights potential in this rapidly evolving field.

Language: Английский

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Long-term monitoring of SARS-CoV-2 variants in wastewater using a coordinated workflow of droplet digital PCR and nanopore sequencing

Water Research, Journal Year: 2024, Volume and Issue: 254, P. 121338 - 121338

Published: Feb. 18, 2024

Quantitative polymerase chain reaction (PCR) and genome sequencing are important methods for wastewater surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The reverse transcription-droplet digital PCR (RT-ddPCR) is a highly sensitive method quantifying SARS-CoV-2 RNA in samples to track the trends viral activity levels but cannot identify new variants. It also takes time develop PCR-based assays targeting variants interest. Whole (WGS) can be used monitor known variants, it generally not quantitative. Several short-read techniques expensive might experience delayed turnaround times when outsourced due inadequate in-house resources. Recently, portable nanopore system offers an affordable real-time wastewater. This technology has potential enable swift response disease outbreaks without relying on clinical results. In addressing concerns related rapid accurate variant analysis, both RT-ddPCR were employed emergence was conducted at 23 sewer maintenance hole sites five treatment plants Michigan from 2020 2022. 2020, dominated by parental (20A, 20C 20G), followed 20I (Alpha, B.1.1.7) early 2021 Delta concern (VOC) late 2021. For year 2022, Omicron dominated. Nanopore validate suspected cases that initially undetermined assays. concordance rate between identifying clade-level 76.9%. Notably, instances disagreement two most prominent identification We showed with N gene concentrations >104 GC/100ml as measured improve recovery coverage depth using MinION device. better detecting key spike protein mutations A67V, del69-70, K417N, L452R, N501Y, N679K, R408S (p-value <0.05) compared sequencing. suggested should coordinated where preliminary quantification confirmatory detection or reported here adopted reliable analysis community level public health response.

Language: Английский

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