Campus node-based wastewater surveillance enables COVID-19 case localization and confirms lower SARS-CoV-2 burden relative to the surrounding community DOI Creative Commons
Jangwoo Lee, Nicole Acosta, Barbara Waddell

et al.

Water Research, Journal Year: 2023, Volume and Issue: 244, P. 120469 - 120469

Published: Aug. 8, 2023

Wastewater-based surveillance (WBS) has been established as a powerful tool that can guide health policy at multiple levels of government. However, this approach not well assessed more granular scales, including large work sites such University campuses. Between August 2021 and April 2022, we explored the occurrence SARS-CoV-2 RNA in wastewater using qPCR assays from complimentary sewer catchments residential buildings spanning Calgary's campus how compared to municipal treatment plant servicing campus. Real-time contact tracing data was used evaluate an association between burden clinically confirmed cases assess potential WBS for disease monitoring across worksites. Concentrations N1 N2 varied significantly six sampling - regardless several normalization strategies with certain consistently demonstrating values 1-2 orders higher than others. Relative clinical identified specific sewersheds, provided one-week leading indicator. Additionally, our comprehensive strategy enabled estimation total per capita, which lower surrounding community (p≤0.001). Allele-specific variants were representative large, no time did emerging first debut on This study demonstrates be efficiently applied locate hotspots activity very scale, predict complex

Language: Английский

Predicting COVID-19 cases using SARS-CoV-2 RNA in air, surface swab and wastewater samples DOI Creative Commons
Helena M. Solo‐Gabriele,

Shelja Kumar,

Samantha Abelson

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 857, P. 159188 - 159188

Published: Oct. 4, 2022

Language: Английский

Citations

29
Wastewater-Based Epidemiology of SARS-CoV-2: Assessing Prevalence and Correlation with Clinical Cases DOI Open Access
Hima Wani,

Smita Menon,

Dipen Desai

et al.

Food and Environmental Virology, Journal Year: 2023, Volume and Issue: 15(2), P. 131 - 143

Published: May 3, 2023

Language: Английский

Citations

21
Wastewater surveillance of SARS-CoV-2 at intra-city level demonstrated high resolution in tracking COVID-19 and calibration using chemical indicators DOI Open Access
Prakit Saingam, Bo Li, Bao Nguyen Quoc

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 866, P. 161467 - 161467

Published: Jan. 7, 2023

Language: Английский

Citations

18
Monkeypox viral nucleic acids detected using both DNA and RNA extraction workflows DOI Creative Commons

Mark Sharkey,

Kristina M. Babler, Bhavarth Shukla

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 890, P. 164289 - 164289

Published: May 20, 2023

Language: Английский

Citations

18
Wastewater based surveillance can be used to reduce clinical testing intensity on a university campus DOI Creative Commons

Ayaaz Amirali,

Kristina M. Babler,

Mark Sharkey

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 918, P. 170452 - 170452

Published: Jan. 29, 2024

Language: Английский

Citations

7
Small-scale wastewater-based epidemiology (WBE) for infectious diseases and antibiotic resistance: A scoping review DOI Creative Commons
Dennis Schmiege, Timo Haselhoff, A. G. R. Thomas

et al.

International Journal of Hygiene and Environmental Health, Journal Year: 2024, Volume and Issue: 259, P. 114379 - 114379

Published: April 15, 2024

Wastewater analysis can serve as a source of public health information. In recent years, wastewater-based epidemiology (WBE) has emerged and proven useful for the detection infectious diseases. However, insights from wastewater treatment plant do not allow small-scale differentiation within sewer system that is needed to analyze target population under study in more detail. Small-scale WBE offers several advantages, but there been no systematic overview its application. The aim this scoping review provide comprehensive current state knowledge on diseases, including methodological considerations A database search was conducted, considering only peer-reviewed articles. Data analyses included quantitative summary qualitative narrative synthesis. Of 2130 articles, we 278, most which were published since 2020. studies analyzed at building level (n = 203), especially healthcare 110) educational facilities 80), neighborhood scale 86). main analytical parameters viruses 178), notably SARS-CoV-2 161), antibiotic resistance (ABR) biomarkers 99), often by polymerase chain reaction (PCR), with DNA sequencing techniques being less common. terms sampling techniques, active dominated. frequent lack detailed information specification selection criteria characterization sites identified concern. conclusion, based large number studies, overarching strategic aspects WBE. An enabling environment requires inter- transdisciplinary sharing across countries. Promoting adoption will benefit common international conceptualization approach, standardized internationally accepted terminology. particular, development good practices different warranted. This includes establishment guidelines local sub-sewersheds, transparent reporting ensure comparability results.

Language: Английский

Citations

7
Comparison of Electronegative Filtration to Magnetic Bead-Based Concentration and V2G-qPCR to RT-qPCR for Quantifying Viral SARS-CoV-2 RNA from Wastewater DOI
Kristina M. Babler,

Ayaaz Amirali,

Mark Sharkey

et al.

ACS ES&T Water, Journal Year: 2022, Volume and Issue: 2(11), P. 2004 - 2013

Published: May 17, 2022

Methods of wastewater concentration (electronegative filtration (ENF) versus magnetic bead-based (MBC)) were compared for the analysis severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), beta-2 microglobulin, and human coronavirus OC43. Using ENF as method, two quantitative polymerase chain reaction (qPCR) analytical methods also compared: volcano second generation (V2G)-qPCR reverse transcriptase (RT)-qPCR measuring three different targets virus responsible COVID-19 illness (N1, modified N3, ORF1ab). Correlations between strong statistically significant SARS-CoV-2 (r = 0.77, p < 0.001) B2M 0.001). Comparison qPCR indicate that, on average, each method provided equivalent results with average ratios 0.96, 1.02 N3 to N1, ORF1ab, N1 ORF1ab supported by (p correlation coefficients 0.67 V2G (N3) RT (N1), r 0.74 (ORF1ab), 0.81 (N1) (ORF1ab)). Overall suggest that provide results, although variability is observed individual measurements. Given equivalency additional advantages disadvantages, described in discussion, are be considered when choosing an appropriate method.

Language: Английский

Citations

26
Wastewater-Based Epidemiology for SARS-CoV-2 Biomarkers: Evaluation of Normalization Methods in Small and Large Communities in Southern Germany DOI

Alexander Mitranescu,

Anna Uchaikina,

Anna‐Sonia Kau

et al.

ACS ES&T Water, Journal Year: 2022, Volume and Issue: 2(12), P. 2460 - 2470

Published: Oct. 10, 2022

In the context of COVID-19 pandemic, wastewater-based epidemiology (WBE) emerged as a useful tool to account for prevalence SARS-CoV-2 infections on population scale. this study, we analyzed wastewater samples from three large (>300,000 people served) and four small (<25,000 communities throughout southern Germany August December 2021, capturing fourth infection wave in dominated by Delta variant (B.1.617.2). As dilution can skew biomarker concentrations wastewater, normalization parameters improve relationship between data clinical data. investigated suitability performance various parameters. Influent flow showed strong relationships precipitation data; accordingly, flow-normalization reacted distinctly events. Normalization surrogate viruses CrAssphage pepper mild mottle virus varying different sampling sites. The best was achieved with mixed fecal indicator calculated both viruses. Analyzing temporal spatial variation proved be explain performance. Overall, our findings indicate that viruses, flow, hydro-chemical is site-specific. We recommend testing individually specific sewage systems.

Language: Английский

Citations

25
Beyond COVID-19: Wastewater-based epidemiology for multipathogen surveillance and normalization strategies DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Niva Sthapit

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 946, P. 174419 - 174419

Published: July 2, 2024

Wastewater-based epidemiology (WBE) is a critical tool for monitoring community health. Although much attention has focused on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causative agent of disease 2019 (COVID-19), other pathogens also pose significant health risks. This study quantified the presence SARS-CoV-2, influenza A virus (Inf-A), and noroviruses genogroups I (NoV-GI) II (NoV-GII) in wastewater samples collected weekly (n = 170) from July 2023 to February 2024 five treatment plants (WWTPs) Yamanashi Prefecture, Japan, by quantitative PCR. Inf-A RNA exhibited localized prevalence with positive ratios 59 %–82 % different WWTPs, suggesting regional outbreaks within specific areas. NoV-GI (94 %, 160/170) NoV-GII (100 170/170) were highly prevalent, (6.1 ± 0.8 log10 copies/L) consistently exceeding (5.4 0.7 concentrations. SARS-CoV-2 was detected 100 samples, mean concentrations 5.3 0.5 copies/L WWTP E 5.8 0.4 each WWTPs. Seasonal variability evident, higher all pathogenic viruses during winter. Non-normalized normalized fecal indicator bacteria (Escherichia coli total coliforms), an (pepper mild mottle (PMMoV)), turbidity revealed associations reported cases. + GII showed strong correlations gastroenteritis cases, particularly when E. (Spearman's ρ 0.70–0.81) coliforms (ρ 0.70–0.81), respectively. For non-normalized correlation 0.61, decreasing 0.31 PMMoV, that PMMoV unsuitable. Turbidity normalization yielded suboptimal results. underscored importance selecting suitable parameters tailored accurate trend using WBE, demonstrating its utility beyond COVID-19 surveillance.

Language: Английский

Citations

5
Piloting wastewater-based surveillance of norovirus in England DOI Creative Commons
David I. Walker,

Jessica Witt,

Wayne Rostant

et al.

Water Research, Journal Year: 2024, Volume and Issue: 263, P. 122152 - 122152

Published: July 29, 2024

Wastewater-based epidemiology (WBE) gained widespread use as a tool for supporting clinical disease surveillance during the COVID-19 pandemic. There is now significant interest in continued development of WBE other pathogens significance. In this study, approximately 3,200 samples wastewater from across England, previously collected quantification SARS-CoV-2, were re-analysed norovirus genogroup I (GI) and II (GII). Overall, GI GII detected 93% 98% respectively, at least one genogroups was 99% samples. found significantly lower concentrations than GII, but proportion each varied over time, with becoming more prevalent some areas towards end study period (May 2021 - March 2022). Using relative strength indices (RSI), it possible to trends genogroup, total time. Increases levels appeared coincide removal related lockdown restrictions within England. Local Moran's analyses indicated several localised outbreaks both notably outbreak north England early 2022. Comparisons national average against concomitant reported case numbers showed linear relationship. This highlights potential wastewater-based monitoring valuable approach support communities.

Language: Английский

Citations

5