Understanding
the
ecotoxicity
potential
of
heavily
used
herbicides
such
as
glyphosate,
glufosinate,
and
aminomethylphosphonic
acid
(AMPA)
is
critically
dependent
on
analytical
analysis
quantification.
This
study
investigated
techniques
for
enhancing
sample
matrix
enrichment,
optimizing
acquisition
protocols,
determining
limits
detection
quantitation
through
offline
solid-phase
extraction
coupled
with
liquid
chromatography
tandem
mass
spectrometry
(LC-MS/MS),
aiming
to
increase
selectivity
sensitivity.
The
most
effective
elution
solution
recovery
all
analytes
from
sorbent
was
100%
MeOH
using
an
Oasis
MCX
cartridge.
effect
flow
rate
at
0.3
mL/min
volume
2
mL
had
a
proportional
after
solid
phase
extraction.
use
optimized
experimental
conditions
resulted
in
about
80-95%
analytes.
simple
efficient
LC-MS/MS
method
routine
testing
glyphosate
AMPA
water
will
enhance
environmental
public
health
studies
usage,
drift,
exposure
these
pesticides.
Methods and Protocols,
Journal Year:
2024,
Volume and Issue:
7(3), P. 38 - 38
Published: May 2, 2024
The
European
Union’s
recent
decision
to
renew
the
authorization
for
use
of
glyphosate
until
15
December
2033
has
stimulated
scientific
discussion
all
around
world
regarding
its
toxicity
or
otherwise
humans.
Glyphosate
is
a
chemical
which
millions
tons
have
been
used
in
last
50
years
worldwide
dry
out
weeds
cultivated
fields
and
greenhouses
on
roadsides.
Concern
raised
many
areas
about
possible
presence
food
chain
consequent
adverse
effects
health.
Both
aspects
that
argue
favor
those
instead
may
indicate
limited
are
discussed
here.
widespread
debate
generated
requires
further
investigations
field
measurements
understand
glyphosate’s
fate
once
dispersed
environment
concentration
chain.
Hence,
there
need
validated
analytical
methods
available
analysts
field.
In
present
review,
determination
main
metabolite,
AMPA,
discussed,
with
specific
focus
chromatographic
techniques
applied
cereal
products.
experimental
procedures
explained
detail,
including
cleanup,
derivatization,
instrumental
conditions,
give
laboratories
involved
enough
information
proceed
implementation
this
line
analysis.
prevalent
LC-MS/MS,
GC-MS/SIM,
GC-MS/MS,
but
sufficient
indications
also
given
wish
better
performing
high-resolution
MS
simpler
HPLC-FLD,
HPLC-UV,
GC-NPD,
GC-FPD
screening
purposes.
concentrations
from
literature
measured
wheat,
corn,
barley,
rye,
oats,
soybean,
cereal-based
foods
reported,
together
regulatory
status
various
parts
accumulation
mechanism.
As
cereals,
data
show
tends
accumulate
more
wholemeal
flours
than
refined
ones,
product
strictly
depends
treatment
period
(the
closer
it
time
harvesting,
higher
concentration),
cold
climates,
herbicide
persist
soil
long
time.
The
European
Union's
recent
decision
to
renew
the
authorization
for
use
of
glyphosate
until
15
December
2033
stimulates
scientific
discussion
all
around
world
regarding
its
toxicity
or
otherwise
humans.
Glyphosate
is
a
chemical
used
by
millions
tons
in
last
50
years
worldwide
drying
weeds
cultivated
fields,
greenhouses,
roadsides.
Concern
has
been
raised
many
quarters
about
possible
presence
food
chain
and
consequent
adverse
effects
on
health:
both
aspects
that
argue
favor
those
instead
may
indicate
limited
are
here
discussed.
great
debate
generated
requires
further
investigations
field
measurements
understand
fate
once
dispersed
environment
concentration
chain.
Hence
need
validated
analytical
methods
available
analysts
field.
In
present
review,
determination
main
metabolite,
AMPA,
discussed,
with
specific
focus
chromatographic
techniques
applied
cereal
products.
experimental
procedures
explained
any
detail,
including
cleanup,
derivatization,
instrumental
conditions
give
laboratories
involved
enough
information
proceed
implementation
this
line
analysis.
prevalent
LC-MS/MS,
GC-MS/SIM,
GC-MS/MS
but
sufficient
indications
also
given
wish
simpler
HPLC-FLD,
HPLC-UV,
GC-NPD
GC-FPD
screening
purposes.
Concentrations
from
literature
measured
wheat,
corn,
barley,
rye,
oats,
soybean,
cereal-based
foods
reported
together
regulatory
status
various
parts
accumulation
mechanism.
As
cereals,
data
show
tends
accumulate
more
wholemeal
flours
than
refined
ones,
product
strictly
depends
treatment
period
(the
closer
it
time
harvesting,
higher
concentration)
cold
climates
herbicide
persist
soil
long
time.
