Coupling pathway prediction and fluorescence spectroscopy to assess the impact of auxiliary substrates on micropollutant biodegradation

Environmental Microbiology, Journal Year: 2024, Volume and Issue: 26(2)

Published: Jan. 17, 2024

Abstract Some bacteria can degrade organic micropollutants (OMPs) as primary carbon sources. Due to typically low OMP concentrations, these may benefit from supplemental assimilation of natural substrates present in the pool dissolved matter (DOM). The biodegradability such auxiliary and impacts on removal are tightly linked biotransformation pathways. Here, we aimed elucidate effect different DOM constituents for carbofuran degrader Novosphingobium sp. KN65.2, using a novel approach that combines pathway prediction, laboratory experiments, fluorescence spectroscopy. Pathway prediction suggested ring hydroxylation reactions catalysed by Rieske‐type dioxygenases flavin‐dependent monooxygenases determine transformability 11 aromatic compounds used model constituents. Our further identified two groups with distinct transformation mechanisms amongst four growth‐supporting selected mixed substrate biodegradation experiments pesticide (Group 1: 4‐hydroxybenzoic acid, 4‐hydroxybenzaldehyde; Group 2: p ‐coumaric ferulic acid). Carbofuran kinetics were stable presence both 1 2 substrates. However, would be preferable bioremediation processes, they showed constant under experimental conditions (pre‐growing KN65.2 vs. constituent). Furthermore, utilisable competitor ( Pseudomonas fluorescens P17). study thus presents simple cost‐efficient reveals mechanistic insights into OMP‐DOM biodegradation.

Language: Английский

Online monitoring of greywater reuse system using excitation-emission matrix (EEM) and K-PARAFACs

Water Research, Journal Year: 2024, Volume and Issue: 268, P. 122604 - 122604

Published: Oct. 11, 2024

Language: Английский

Analytical Challenges in the Water Reuse Scenario

Lecture notes in chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 367 - 391

Published: Jan. 1, 2024

Language: Английский