High-Level De Novo Production of (2S)-Naringenin in Yarrowia lipolytica Using Metabolic and Enzyme Engineering

ACS Agricultural Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Language: Английский

Metabolic engineering ofAcinetobacter baylyiADP1 for naringenin production

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 6, 2024

Abstract Naringenin, a flavanone and precursor for variety of flavonoids, has potential applications in the health pharmaceutical sectors. The biological production naringenin using genetically engineered microbes is considered as promising strategy. synthesis pathway involving chalcone synthase (CHS) isomerase (CHI) relies on efficient supply key substrates, malonyl-CoA coumaroyl-CoA. In this research, we utilized soil bacterium, Acinetobacter baylyi ADP1, which exhibits several characteristics that make it suitable candidate biosynthesis; strain naturally tolerates can uptake metabolize coumarate, primary compound alkaline-pretreated lignin production. A. ADP1 also produces intracellular lipids, such wax esters, thereby being able to provide an excess biosynthesis. Moreover, genomic engineering notably straightforward. course construction naringenin-producing strain, coumarate catabolism was eliminated by single gene knockout (Δ hcaA ) various combinations plant-derived CHS CHI were evaluated. best performance obtained novel combination genes encoding from Hypericum androsaemum Medicago sativa, enabled 18 mg/L batch cultivations coumarate. Furthermore, implementation fed-batch system led significant 3.7-fold increase (66 mg/L) These findings underscore host biosynthesis well advancement lignin-based bioproduction.

Language: Английский

Metabolic engineering of Acinetobacter baylyi ADP1 for naringenin production

Metabolic Engineering Communications, Journal Year: 2024, Volume and Issue: 19, P. e00249 - e00249

Published: Nov. 1, 2024

Language: Английский