Defence Warriors: Exploring the crosstalk between polyamines and oxidative stress during microbial pathogenesis DOI Creative Commons
Abhilash Vijay Nair, Anmol Singh, Dipshikha Chakravortty

et al.

Redox Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103648 - 103648

Published: April 1, 2025

Microbial infections have been a widely studied area of disease research since historical times, yet they are cause severe illness and deaths worldwide. Furthermore, by pathogens not just restricted to humans; instead, diverse range hosts, including plants, livestock, marine organisms fish, significant economic losses pose threats humans through their transmission in the food chain. It is now believed that both pathogen host contribute outcomes pathology. Researchers unravelled numerous aspects host-pathogen interactions, offering valuable insights into physiological, cellular molecular processes factors development infectious diseases. Polyamines key regulating human ageing health. However, often overlooked context interactions despite playing dynamic role as defence molecule from perspective well pathogen. They form complex network interacting with several molecules within cell, reactive oxygen species being component. This review presents thorough overview current knowledge polyamines intricate infection multiple hosts. Interestingly, covers interplay commensals involving species, highlighting an unexplored this field. From future perspective, oxidative stress microbial pathogenesis fascinating widens scope developing therapeutic strategies combat deadly infections.

Language: Английский

Putrescine Depletion in Leishmania donovani Parasites Causes Immediate Proliferation Arrest Followed by an Apoptosis-like Cell Death DOI Creative Commons

John W. Johnston,

Jonathan Taylor,

Surbhi Nahata

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(2), P. 137 - 137

Published: Feb. 2, 2025

The polyamine pathway in Leishmania parasites has emerged as a promising target for therapeutic intervention, yet the functions of polyamines remain largely unexplored. Ornithine decarboxylase (ODC) and spermidine synthase (SPDSYN) catalyze sequential conversion ornithine to putrescine spermidine. We previously found that donovani Δodc Δspdsyn mutants exhibit markedly reduced growth vitro diminished infectivity mice, with effect being most pronounced putrescine-depleted mutants. Here, we report that, polyamine-free media, ∆odc arrested proliferation replication, while ∆spdsyn showed slow replication phenotype. Starved also exhibited marked reduction metabolism, which was not observed starved cells. In contrast, both displayed mitochondrial membrane hyperpolarization. Hallmarks apoptosis, specifically DNA fragmentation modifications, were incubated media. These results show depletion had an immediate detrimental on cell growth, metabolism caused apoptosis-like death Our findings establish ODC within biosynthetic treating leishmaniasis.

Language: Английский

Citations

0
Defence Warriors: Exploring the crosstalk between polyamines and oxidative stress during microbial pathogenesis DOI Creative Commons
Abhilash Vijay Nair, Anmol Singh, Dipshikha Chakravortty

et al.

Redox Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103648 - 103648

Published: April 1, 2025

Microbial infections have been a widely studied area of disease research since historical times, yet they are cause severe illness and deaths worldwide. Furthermore, by pathogens not just restricted to humans; instead, diverse range hosts, including plants, livestock, marine organisms fish, significant economic losses pose threats humans through their transmission in the food chain. It is now believed that both pathogen host contribute outcomes pathology. Researchers unravelled numerous aspects host-pathogen interactions, offering valuable insights into physiological, cellular molecular processes factors development infectious diseases. Polyamines key regulating human ageing health. However, often overlooked context interactions despite playing dynamic role as defence molecule from perspective well pathogen. They form complex network interacting with several molecules within cell, reactive oxygen species being component. This review presents thorough overview current knowledge polyamines intricate infection multiple hosts. Interestingly, covers interplay commensals involving species, highlighting an unexplored this field. From future perspective, oxidative stress microbial pathogenesis fascinating widens scope developing therapeutic strategies combat deadly infections.

Language: Английский

Citations

0