Self-Assembled DNA/SG-I Nanoflower: Versatile Photocatalytic Biosensors for Disease-Related Markers

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

DNA nanostructures have recently attracted more attention with functionalities, programmability, and biocompatibility. Herein, a novel self-assembled photocatalytic DNA/SYBR Green I (SG-I) nanoflower (DSNF) was successfully synthesized by rolling circle amplification. DSNF through liquid crystallization of high concentration in the RCA products, without relying on Watson-Crick base-pairing principle. Interestingly, not only possessed larger specific surface area good stability but also exhibited excellent activity that generates singlet oxygen superoxide anion to oxidate 3,3',5,5'-tetramethylbenzidine. Meanwhile, combined an enzyme-linked immunosorbent assay develop new colorimetric sensor for highly specific, sensitive, visual detection carcinoembryonic antigens (CEAs). The achieved sensitive low-cost quantitative CEA linear range 0.5-80.0 ng/mL, LOD 0.5 ng/mL. In addition, three negative seven positive clinical serum samples were obtained 100% accuracy using proposed sensor, showing great potential application cancer diagnosis. We envision this is expected provide important perspectives fluorescence imaging, photosensitizing therapy, diagnosis fields.

Language: Английский

Exploring therapeutic and diagnostic potential of cysteine cathepsin as targets for cancer therapy with nanomedicine

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 144324 - 144324

Published: May 1, 2025

Language: Английский