Alzheimer's disease pathophysiology in the Retina

Progress in Retinal and Eye Research, Journal Year: 2024, Volume and Issue: 101, P. 101273 - 101273

Published: May 15, 2024

The retina is an emerging CNS target for potential noninvasive diagnosis and tracking of Alzheimer's disease (AD). Studies have identified the pathological hallmarks AD, including amyloid β-protein (Aβ) deposits abnormal tau protein isoforms, in retinas AD patients animal models. Moreover, structural functional vascular abnormalities such as reduced blood flow, Aβ deposition, blood-retinal barrier damage, along with inflammation neurodegeneration, been described mild cognitive impairment dementia. Histological, biochemical, clinical studies demonstrated that nature severity pathologies brain correspond. Proteomics analysis revealed a similar pattern dysregulated proteins biological pathways patients, enhanced inflammatory neurodegenerative processes, impaired oxidative-phosphorylation, mitochondrial dysfunction. Notably, investigational imaging technologies can now detect AD-specific deposits, well vasculopathy neurodegeneration living suggesting alterations at different stages links to pathology. Current exploratory ophthalmic modalities, optical coherence tomography (OCT), OCT-angiography, confocal scanning laser ophthalmoscopy, hyperspectral imaging, may offer promise assessment AD. However, further research needed deepen our understanding AD's impact on its progression. To advance this field, future require replication larger diverse cohorts confirmed biomarkers standardized retinal techniques. This will validate aiding early screening monitoring.

Language: Английский

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Ferritin nanocage-enabled detection of pathological tau in living human retinal cells

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 21, 2024

Tauopathies, including Alzheimer's disease and Frontotemporal Dementia, are debilitating neurodegenerative disorders marked by cognitive decline. Despite extensive research, achieving effective treatments significant symptom management remains challenging. Accurate diagnosis is crucial for developing therapeutic strategies, with hyperphosphorylated protein units tau oligomers serving as reliable biomarkers these conditions. This study introduces a novel approach using nanotechnology to enhance the diagnostic process tauopathies. We developed humanized ferritin nanocages, nanoscale delivery system, designed encapsulate transport tau-specific fluorophore, BT1, into human retinal cells detecting neurofibrillary tangles in tissue, key marker of The BT1 living was successfully achieved through demonstrating efficient encapsulation derived from induced pluripotent stem cells. Our experiments confirmed colocalization pathological forms cells, highlighting method's potential identifying Using nanocages represents contribution nanobiotechnology, particularly diagnostics. method offers promising tool early detection implications improving exemplifies integration biomedical science, expanding frontiers nanomedicine techniques.

Language: Английский

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BODIPY in Alzheimer's disease diagnostics: A review

European Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 276, P. 116682 - 116682

Published: July 20, 2024

Language: Английский

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Ferritin Nanocage-Enabled Detection of Pathological Tau in Living Human Retinal Cells

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Background Tauopathies, such as Alzheimer's disease and Frontotemporal Dementia, are debilitating neurodegenerative disorders characterized by cognitive decline. Despite extensive research, effective treatments significant advancements in managing symptoms have been challenging to achieve. Accurate diagnosis is critical for developing therapeutic strategies. Hyperphosphorylated protein units tau oligomers recognized reliable biomarkers these conditions. This study introduces an innovative approach using nanotechnology enhance the diagnostic process tauopathies. We focus on development application of humanized ferritin nanocages, a novel nanoscale delivery system, designed encapsulate transport tau-specific fluorophore, BT1, into human retinal cells, detection neurofibrillary tangles tissue, key marker Results The BT1 living cells was achieved through use system at nanoscale. nanocages demonstrated efficient encapsulation derived from induced pluripotent stem cells. Our experiments successful colocalization with pathological forms highlighting potential this method identifying Conclusions employment probe represents important contribution field nanobiotechnology, especially context diagnostics. offers promising tool early implications improving management exemplifies integration biomedical science, expanding frontiers nanomedicine techniques.

Language: Английский

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Isophorone-based crystallization-induced-emission sensors detect proteome aggregation in live cells and tissues with breast cancer

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1317, P. 342916 - 342916

Published: June 26, 2024

Protein misfolding and aggregation can lead to various diseases. Recent studies have shed light on the aggregated protein in breast cancer pathology, which suggests that it is crucial design chemical sensors visualize aggregates cancer, especially clinical patient-derived samples. However, most reported are constrained cultured cell lines.

Language: Английский

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