A novel fluorescence biosensor based on CRISPR/Cas12a integrated MXenes for detecting Aflatoxin B1

Talanta, Journal Year: 2022, Volume and Issue: 252, P. 123773 - 123773

Published: Aug. 6, 2022

Aflatoxin B1 (AFB1) contamination in food threatens global safety, and rapid quantitative detection of AFB1 remains a challenge. Herein, novel fluorescence biosensor was developed for based on CRISPR/Cas12a MXenes. Specifically, the well-designed activator locked by dual-AFB1 aptamers, Cas12a directly linked to crRNA form inactivated complexes, MXenes efficiently adsorbed FAM fluorophore-modified single-stranded DNA (ssDNA-FAM), quenching its fluorescence. In presence AFB1, released due preferential binding aptamer then activated trans-cleavage activity indiscriminately cleave ssDNA MXenes, leading recovery signal. The fluorescent had wide range from 0.001 80 ng mL-1, limit 0.92 pg ability detect within min. More importantly, platform demonstrates excellent performance real peanut samples.

Language: Английский

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Exogenous calcium oxide nanoparticles alleviate cadmium toxicity by reducing Cd uptake and enhancing antioxidative capacity in barley seedlings

Journal of Hazardous Materials, Journal Year: 2022, Volume and Issue: 438, P. 129498 - 129498

Published: June 29, 2022

Language: Английский

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Nanocellulose-based polymeric nanozyme as bioinspired spray coating for fruit preservation

Food Hydrocolloids, Journal Year: 2022, Volume and Issue: 135, P. 108138 - 108138

Published: Sept. 15, 2022

The growing concerns about microbial food safety and environmental contamination call for sustainable active packaging materials practices with high performance. This contribution proposed a biocatalytic spraying coating fruit preservation using carboxymethyl cellulose nanofibers grafting enzyme-like metal-organic frameworks ([email protected]) banana mango as model fruits. well-characterized biohybrid were verified to simultaneously mimic good oxidase-like apyrase-like activities, enabling effective sterilization against three typical foodborne pathogens, i.e. Escherichia coli O157: H7, Staphylococcus aureus, methicillin-resistant reaching killing rates 90.46%. biocidal •O2− generation adenosine triphosphate depletion the possible mechanisms causing nonspecific bacteria death. Such antibacterial performance, well additional protective properties of [email protected], facilitated quality during 12-day storage bananas mangos by spray biomimetic protected] It is expected that this work walks out first step nanozyme toward engineering fields food, agriculture, biomedicine.

Language: Английский

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Bioengineered chitosan-iron nanocomposite controls bacterial leaf blight disease by modulating plant defense response and nutritional status of rice (Oryza sativa L.)

Nano Today, Journal Year: 2022, Volume and Issue: 45, P. 101547 - 101547

Published: July 5, 2022

Language: Английский

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Mesoporous silica coated core-shell nanoparticles substrate for size-selective SERS detection of chloramphenicol

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2022, Volume and Issue: 284, P. 121817 - 121817

Published: Aug. 30, 2022

With the growing popularity of non-destructive technique, surface-enhanced Raman spectroscopy (SERS) demands a highly sensitive and reproducible plasmonic nanoparticles substrate. In this study, novel bimetallic core-shell (Au@Ag@mSiO2NP) substrate consisting gold core, silver shell, mesoporous silica coating was synthesized. The structure created by employing template molecules such as surfactant their subsequent removal allowing selective screening based on size analyte molecules. Results showed that could selectively enhance small preventing large macromolecules to reach exciting zone achieving detection chloramphenicol in milk samples with limit 6.68 × 10-8 M. Moreover, provided additional stability Au@Ag nanoparticles, leading reusability Thus, work offered simple smart Au@Ag@mSiO2NP for effective SERS analytes.

Language: Английский

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Bacillus spp. as Bioagents: Uses and Application for Sustainable Agriculture

Biology, Journal Year: 2022, Volume and Issue: 11(12), P. 1763 - 1763

Published: Dec. 5, 2022

Food security will be a substantial issue in the near future due to expeditiously growing global population. The current trend agriculture industry entails extravagant use of synthesized pesticides and fertilizers, making sustainability difficult challenge. Land degradation, lower production, vulnerability both abiotic biotic stresses are problems caused by usage these fertilizers. major goal sustainable is ameliorate productivity reduce pests disease prevalence such degree that prevents large-scale damage crops. Agriculture composite interrelation among plants, microbes, soil. Plant microbes play role growth promotion improve soil fertility as well. Bacillus spp. produces an extensive range bio-chemicals assist plant control, promote development, make them suitable for agricultural uses. support N fixation, P K solubilization, phytohormone synthesis, addition being most propitious biocontrol agent. Moreover, Bacilli excrete extracellular metabolites, including antibiotics, lytic enzymes, siderophores, demonstrate antagonistic activity against phytopathogens. boosts resistance toward pathogens inducing systemic (ISR). effective microbial insecticide insects thuringiensis (Bt). Additionally, incorporation toxin genes genetically modified crops increases pests. There constant increase identified species potential agents. they have been involved biosynthesis metallic nanoparticles. main objective this review article display uses application specie promising biopesticide agriculture. strains yield attributes could valuable developing novel formulations lead way

Language: Английский

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Nanocomposite hydrogel for daytime passive cooling enabled by combined effects of radiative and evaporative cooling

Chemical Engineering Journal, Journal Year: 2022, Volume and Issue: 457, P. 141231 - 141231

Published: Dec. 29, 2022

Language: Английский

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Green synthesis of metal-based nanoparticles for sustainable agriculture

Environmental Pollution, Journal Year: 2022, Volume and Issue: 309, P. 119755 - 119755

Published: July 12, 2022

Language: Английский

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Bio-interface engineering of MXene nanosheets with immobilized lysozyme for light-enhanced enzymatic inactivation of methicillin-resistant Staphylococcus aureus

Chemical Engineering Journal, Journal Year: 2022, Volume and Issue: 452, P. 139078 - 139078

Published: Sept. 8, 2022

Being a unique biocatalyst to hydrolyse structural polysaccharides on the cell wall of bacteria, lysozyme can slow down bacterial resistance caused by antibiotic overdose treatments, however, efficient activation activities under pathogenic microenvironments remains challenging. Herein, bio-interface engineering strategy was proposed for remote modulation thermoresistant upon rationally designed photothermal nanoplatforms. For this, Ti3C2TX MXene nanosheets were functionalized polydopamine (PDA) surface chemistry enhance effects and performance durability, during which biomacromolecules immobilized at such two-dimensional hybrid interface via intermolecular electrostatic affinity. The integrated nanoplatform (denoted as M@P@Lyso), with an optimal high light-to-heat conversion efficiency 46.88%, realized not only precision control local heat but also photo-responsive up-regulation bio-catalysis laden lysozyme. As result, in vitro vivo antibacterial experiments revealed that M@P@Lyso could effectively inhibit proliferation methicillin-resistant Staphylococcus aureus accelerated wound disinfection mice negligible biological toxicities. outstanding attributed photo-enhanced activity, assisted death mild hyperthermia physical destruction derived from M@PDA. This work exemplified solution threats stimuli-responsive enzymatic

Language: Английский

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Bio-Inspired Nanomaterials for Micro/Nanodevices: A New Era in Biomedical Applications

Micromachines, Journal Year: 2023, Volume and Issue: 14(9), P. 1786 - 1786

Published: Sept. 18, 2023

Exploring bio-inspired nanomaterials (BINMs) and incorporating them into micro/nanodevices represent a significant development in biomedical applications. Nanomaterials, engineered to imitate biological structures processes, exhibit distinctive attributes such as exceptional biocompatibility, multifunctionality, unparalleled versatility. The utilization of BINMs demonstrates potential diverse domains micro/nanodevices, encompassing biosensors, targeted drug delivery systems, advanced tissue engineering constructs. This article thoroughly examines the various BINMs, including those originating from proteins, DNA, biomimetic polymers. Significant attention is directed toward these entities subsequent ramifications that arise. review explores biomimicry’s structure–function correlations. Synthesis mosaics include bioprocesses, biomolecules, natural structures. These nanomaterials’ interfaces use functionalization geometric adaptations, transforming delivery, nanobiosensing, organ-on-chip cancer-on-chip models, wound healing dressing mats, antimicrobial surfaces. It provides an in-depth analysis existing challenges proposes prospective strategies improve efficiency, performance, reliability devices. Furthermore, this study offers forward-thinking viewpoint highlighting avenues for future exploration advancement. objective effectively utilize maximize application progression thereby propelling rapidly developing field its promising future.

Language: Английский

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