LOC646762 Is Involved in Adipogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells DOI Creative Commons

Yifan Shao,

Minqi Xiong,

Jianyun Liu

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 6, 2024

Long noncoding RNA (lncRNA) has been shown to participate in adipogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). In this study, we aimed investigate the role lncRNA-LOC646762 BMSCs. Transcriptome sequencing revealed a positive correlation between LOC646762 transcription and expression marker genes differentiation. Moreover, overexpression did not negatively impact cell proliferation Besides, plays crucial differentiation, as evidenced by its with gene expression. Its possible interaction proposed target C/EBPβ suggests involvement essential pathways governing adipogenesis. Collectively, our study outcomes provide valuable insights into molecular mechanisms underlying BMSCs lay strong foundation for further research regenerative medicine.

Language: Английский

MVP-LCN2 axis triggers evasion of ferroptosis to drive hepatocarcinogenesis and sorafenib resistance DOI
Jiawen Xu, Bo Wang, Qiaoyu Liu

et al.

Drug Resistance Updates, Journal Year: 2025, Volume and Issue: 81, P. 101246 - 101246

Published: April 17, 2025

Language: Английский

Citations

1
Targeting cell death mechanisms: the potential of autophagy and ferroptosis in hepatocellular carcinoma therapy DOI Creative Commons
Beibei Liu,

Ling Liu,

Yang Liu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 9, 2024

Ferroptosis is a type of cell death that plays remarkable role in the growth and advancement malignancies including hepatocellular carcinoma (HCC). Non-coding RNAs (ncRNAs) have considerable impact on HCC by functioning as either oncogenes or suppressors. Recent research has demonstrated non-coding ability to control ferroptosis cells, hence impacting tumors resistance these cells drugs. Autophagy mechanism conserved throughout evolution maintaining balance body under normal settings. Nevertheless, occurrence dysregulation autophagy evident progression various human disorders, specifically cancer. dual roles cancer, potentially influencing both survival death. prevalent kind liver genetic mutations changes molecular pathways might worsen its advancement. The subject debate, it capacity repress promote tumor growth. activation can apoptosis, proliferation glucose metabolism, facilitate spread through EMT. Inhibiting hinder enhance respond treatment. regulated several signaling pathways, such STAT3, Wnt, miRNAs, lncRNAs, circRNAs. Utilizing anticancer drugs target may advantageous implications for efficacy cancer

Language: Английский

Citations

6
5-methylcytosine methylation of MALAT1 promotes resistance to sorafenib in hepatocellular carcinoma through ELAVL1/SLC7A11-mediated ferroptosis DOI Creative Commons
Chuan-Jian Shi,

Fengxiang Pang,

Yuhe Lei

et al.

Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 78, P. 101181 - 101181

Published: Dec. 4, 2024

Emerging evidence demonstrates that long non-coding RNAs (lncRNAs) play a crucial role in sorafenib resistance hepatocellular carcinoma (HCC), and lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is dysregulated sorafenib-resistant HCC cells. However, the underlying regulatory mechanisms of MALAT1 cells remain unclear. In present study, we demonstrated 5-methylcytosine (m5C) methylation catalyzed by NSUN2 ALYREF contributed to RNA stability upregulation MALAT1. The NSUN2/ALYREF/MALAT1 signaling axis was activated cells, inhibited sorafenib-induced ferroptosis drive resistance. Mechanistically, maintained mRNA SLC7A11 directly binding ELAVL1 stimulating its cytoplasmic translocation. Furthermore, explored new synergetic strategy for treatment combining inhibitor MALAT1-IN1 with sorafenib. results significantly enhanced efficacy both vitro vivo. Collectively, our work brings insights into epigenetic offers an alternative therapeutic targeting patients.

Language: Английский

Citations

5
Phase separation of RNF214 promotes the progression of hepatocellular carcinoma DOI Creative Commons
Zhengjie He, Ke He, Songwang Cai

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(7)

Published: July 5, 2024

Abstract Hepatocellular carcinoma (HCC) is one of the most common malignant tumors, and expression function an uncharacterized protein RNF214 in HCC are still unknown. Phase separation has recently been observed to participate progression HCC. In this study, we investigated expression, function, phase We found that was highly expressed associated with poor prognosis. functioned as oncogene promote proliferation, migration, metastasis Mechanically, underwent separation, coiled-coil (CC) domain mediated its separation. Furthermore, CC necessary for oncogenic Taken together, our data favored promoted may be a potential biomarker therapeutic target

Language: Английский

Citations

4
The liver-specific long noncoding RNA FAM99B inhibits ribosome biogenesis and cancer progression through cleavage of dead-box Helicase 21 DOI Creative Commons
Yifei He, Hongquan Li,

Qili Shi

et al.

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 14, 2025

Abstract Emerging evidence has demonstrated that long noncoding RNAs (lncRNAs) are promising targets or agents for the treatment of human cancers. Most liver-specific lncRNAs exhibit loss expression and act as tumor suppressors in liver cancer. Modulating these is a potential approach lncRNA-based gene therapy hepatocellular carcinoma (HCC). Here, we report lncRNA FAM99B significantly decreased HCC tissues suppresses cell proliferation metastasis both vitro vivo. promotes nuclear export DDX21 through XPO1, leading to further cleavage by caspase3/6 cytoplasm. inhibits ribosome biogenesis inhibiting ribosomal RNA (rRNA) processing RPS29/RPL38 transcription, thereby reducing global protein synthesis downregulation cells. Interestingly, 65-146 truncation exhibits tumor-suppressive effects vivo vitro. Moreover, GalNAc-conjugated growth orthotopic xenografts, providing new strategy HCC. This first use an agent rather than target treatment.

Language: Английский

Citations

0
Crosstalk Between Phase-Separated Membraneless Condensates and Membrane-Bound Organelles in Cellular Function and Disease DOI

Aydan Torun,

Hoşnaz Tuğral,

Sreeparna Banerjee

et al.

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
The role of non-coding RNA in ferroptosis of liver cancer and its impact on lipid peroxidation DOI Creative Commons

Minglu Ding,

Keyuan Huo,

Xiaojie Chen

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

Ferroptosis is an iron-dependent programmed death caused by the imbalance of lipid peroxides in cells. Unlike apoptosis, autophagy and necrosis, ferroptosis mainly induced small molecule compound erastin. The main characteristics were glutathione (GSH) depletion, inactivation peroxidase 4 (GPX4) reactive oxygen species (ROS) promoting peroxidation. Eventually, peroxidation regulation cells leads to ferroptosis. metabolic pathway ultimately contributes through production peroxides. In addition, other cellular pathways can also regulate ferroptosis, such as antioxidant pathway, which inhibits clearing reducing cell membrane damage. Long non-coding RNAs (lncRNAs) are transcripts more than 200 nucleotides length a less classified group RNA that associated with tumorigenesis metastasis tissue or type specific protein-coding genes. Studies on molecular profile lncRNAs plasma samples from liver cancer patients show differentially expressed concentrated biological functions related tumorigenesis, metastasis, immune response regulation. With different physiological pathological environments, expression patterns coordinate state, development, differentiation, disease.

Language: Английский

Citations

0
Membraneless Organelles and Phase Separation in Tumours: Mechanisms and Prospects DOI Creative Commons

Yang Hao,

Chu Zhong,

Shuwen Han

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

ABSTRACT Membraneless organelles (MLOs) are a type of subcellular compartment structure discovered in eukaryotes recent years. They mainly formed through the liquid–liquid phase separation (LLPS) and aggregation macromolecular substances such as proteins or nucleic acids cells. When cells stimulated, they initiate series stress responses including gene transcription, RNA metabolism, translation, protein modification signal transduction to maintain homeostasis. The dysregulation these cellular processes is key event occurrence development cancer. This article provides an overview function membraneless organelles, well mechanisms separation, summarise latest research progress on tumours. It focuses role molecular mechanism LLPS tumours, with aim providing new theoretical references for developing drug action targets innovative treatment strategies.

Language: Английский

Citations

0
Epigenetic and Post-Translational Modifications in Ferroptosis Regulation and Hepatocellular Carcinoma: New Frontiers in Therapeutic Targeting DOI

Soheil Bolandi,

Samaneh Dodge,

Zahra Zahed

et al.

Pathology - Research and Practice, Journal Year: 2025, Volume and Issue: unknown, P. 155991 - 155991

Published: April 1, 2025

Language: Английский

Citations

0
SP1 undergoes phase separation and activates RGS20 expression through super-enhancers to promote lung adenocarcinoma progression DOI Creative Commons

Liying Shan,

Wenmeng Wang, Lijuan Du

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(29)

Published: July 8, 2024

Lung adenocarcinoma (LUAD) is the leading cause of cancer-related death worldwide, but underlying molecular mechanisms remain largely unclear. The transcription factor (TF) specificity protein 1 (SP1) plays a crucial role in development various cancers, including LUAD. Recent studies have indicated that master TFs may form phase-separated macromolecular condensates to promote super-enhancer (SE) assembly and oncogene expression. In this study, we demonstrated SP1 undergoes phase separation its zinc finger 3 DNA-binding domain essential for process. Through Cleavage Under Targets & Release Using Nuclease (CUT&RUN) using antibodies against H3K27ac, found significant correlation between enrichment SE elements, identified regulator G signaling 20 (RGS20) gene as most likely target regulated by through mechanisms, verified finding different approaches. oncogenic activity relies on ability RGS20 activation, which can be abolished glycogen synthase kinase J4 (GSK-J4), demethylase inhibitor. Together, our findings provide evidence regulates expression thereby promoting LUAD cell progression. This study also revealed an innovative therapies intervening SP1-mediated formation.

Language: Английский

Citations

3