Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: May 17, 2025
Language: Английский
Zoonoses, Journal Year: 2024, Volume and Issue: 4(1)
Published: Jan. 1, 2024
Disease X stands as a symbol for subset of emerging infectious diseases rising to prominence significant challenge global health security. This comprehensive review delves into the historical context, precise definition, and illustrative case studies disease X, including notable examples, such COVID-19, SARS, Ebola. The discussion then transitions an examination potential origins with particular focus on zoonotic pathways influence environmental factors. concludes suite proposed strategies aimed at prevention containment emphasizing critical role vigilant surveillance, swift response mechanisms, necessity fostering international cooperation.
Language: Английский
Citations
4
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 494, P. 152885 - 152885
Published: June 5, 2024
Language: Английский
Citations
4
European Journal of Medicinal Chemistry Reports, Journal Year: 2024, Volume and Issue: unknown, P. 100238 - 100238
Published: Oct. 1, 2024
Language: Английский
Citations
4
Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: unknown, P. 100736 - 100736
Published: Jan. 1, 2025
Language: Английский
Citations
0
Poultry Science, Journal Year: 2025, Volume and Issue: 104(4), P. 104924 - 104924
Published: Feb. 19, 2025
Since March 2013, the H7N9 subtype of avian influenza virus (AIV) has become an important zoonotic infectious disease, garnering significant global attention because its potential to affect human health. Establishing a rapid, effective, and sensitive method detect H7 AIVs is crucial for disease control. In this study, we developed graphene oxide multilayer quantum dot-based immunochromatographic strip ultrasensitive detection AIVs. The demonstrated excellent sensitivity, with limit 0.063 hemagglutinin units 0.016 ng/ml protein. exhibited remarkable specificity, no reaction other subtypes A andno cross-reactivity types virus. Additionally, reproducibility, both inter-group intra-group variations remaining below 10 %. Preliminary testing on clinical samples showed impressive consistency, underscoring method's reliability. These initial results suggest that approach widespread use in analyzing samples, indicating substantial promise future application various diagnostic settings.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: March 5, 2025
We designed, developed, and clinically tested two rapid antigen-based immunosensors for SARS-CoV-2 detection, enabling diagnosis viral load quantification under USD $2. In a first clinical study, screen-printed disposable carbon-based (SPC) sensor was assessed on prospectively recruited adult participants classified into three study groups: healthy donors (n = 46); SARS-CoV-2-infected symptomatic patients 58); co-habitants of without prior testing 38). Nasopharyngeal aspirates (NA), oropharyngeal swabs (OS), saliva (SA) samples were obtained from all participants. Performance measured in terms sensitivity specificity against reference diagnostic RT-qPCR kit analytical (limit LoD) using recombinant material lab tests. A second performed the same design, albeit with laser-induced graphene (LIG) electrodes, nasopharyngeal (NS) 224 patient at different stages pandemic, which 110 negative 114 positive via RT-qPCR. find OS most informative sample, when compared to NA SA. The SPC-based sensors had 93.8% 61.5% samples, while LIG-based NS lower 68.93%, significantly higher 86.17%. believe values SPC driven by results affected low (75.5%) high LoD (> 20,000 copies/mL) used, due reduced set effective antigen-binding sites caused non-covalent LIG-mAb ligands used. immunosensor's spike protein phosphate-buffered saline (PBS) both types near 1 fg/mL showed no cross-reactivity structural proteins Epstein-Barr Influenza. metrics time-to-result (5 < 12 min) provide proof-of-principle applicability as low-cost, technology determining infections. Changing working electrode LIG, instead SPC, improved even presence pathogen variants. Discordant between our immunosensor versions tests are attributed not only limited antibody effectiveness former but also quality probes used height pandemic.
Language: Английский
Citations
0
JOM, Journal Year: 2025, Volume and Issue: unknown
Published: March 14, 2025
Language: Английский
Citations
0
ChemistrySelect, Journal Year: 2025, Volume and Issue: 10(13)
Published: April 1, 2025
Abstract The COVID‐19 pandemic outbreak raised major concerns in public health globally, raising the crucial need for development of methods to monitor spread communities worldwide. Wastewater‐based epidemiology (WBE) surveillance has been used as a novel tool outbreaks because its affordability and efficiency tracking infectious contaminants. Unlike other means tracking, wastewater is independent individuals having accessibility healthcare, doctor visits, or infection testing availability. Consequently, considerable awareness complete infections, including at community level. In WBE studies, polymerase chain reaction‐based (PCR) techniques are referred “gold‐standard” method detecting SARS‐CoV‐2 many countries. Nevertheless, despite extensive sensitive selective PCR‐based methods, these have shown some limitations that hinder their application, such requirement repeated heating cooling cycles analysis time 3–4 h. alternative do not rely on same consumables conventionally employed electrochemical biosensing, environmental water samples offers favorable advantages improved turnaround times portability. However, currently highly focused clinical applications than wastewater. This review focuses disadvantages associated with conventional alternative: electrochemical‐bioreceptor‐based technique SARS‐CoV‐2. addition, highlights broad use WBE, binding affinity various bioreceptors toward viral proteins, enhancing analytical properties biosensors integration techniques. integrated systems, especially, electrochemical‐CRISPR based, high sensitivities (down concentrations atto‐molar), potential application low‐resource areas.
Language: Английский
Citations
0
Respiratory Research, Journal Year: 2025, Volume and Issue: 26(1)
Published: April 15, 2025
Pulmonary hypertension (PH) is a serious disease characterized by elevated pulmonary artery pressure, with its prevalence and incidence continuously increasing, posing threat to the lives of many patients worldwide. Due complex etiology PH lack specificity in clinical manifestations, there currently effective specific methods for early diagnosis practice. Biosensors hold significant promise detection, therapeutic monitoring, prognostic evaluation, personalized treatment PH, owing their rapid, sensitive, highly selective characteristics. The rapid development various types biosensors, such as electrochemical optical microfluidic wireless combined use nanomaterials, makes accurate detection PH-related biomarkers possible. Despite broad application prospects biosensors field challenges remain terms sensitivity, selectivity, stability, regulation. This article reviews main pathophysiological mechanisms commonly used principles summarizes progress research well current challenges, order promote further in-depth biosensor technology, thereby improving effects PH. Clinical trial number: Not applicable.
Language: Английский
Citations
0
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: April 22, 2025
The development of portable, cost-effective, and highly sensitive biosensors for real-time biomarker detection is crucial advancing point-of-care testing (POCT) wearable health monitoring. Here, we present an integrated portable electrochemical sensor (ip-ECS) that combines gold nanoparticles (AuNPs) MXene-modified screen-printed electrodes (SPEs) with a custom-designed, low-power electronic system monitoring serum biomarkers. AuNPs MXene nanocomposite significantly enhances the performance SPE by providing high density active sites, improved conductivity, catalytic activity. two model molecules (DA UA) validated feasibility ip-ECS, achieving limits as low 1.12 1.11 μM UA DA, respectively. Furthermore, ip-ECS was successfully applied to detect Cys C in human serum, showing linear response range 50-5000 ng/mL strong correlation (ρ = 0.9556) conventional latex immunoturbidimetry (LIA). Clinical validation using samples from pregnant women revealed elevated levels gestational diabetes mellitus (GDM) patients, highlighting sensor's potential early GDM risk prediction. represents significant step forward next-generation POCT, diagnostics, personalized medicine.
Language: Английский
Citations
0