Activatable prodrug for controlled release of an antimicrobial peptide via the proteases overexpressed inCandida albicansandPorphyromonas gingivalis DOI Open Access
Lubna Amer, Maurice Retout, Jesse V. Jokerst

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 27, 2023

ABSTRACT We report the controlled release of an antimicrobial peptide using enzyme-activatable prodrugs to treat and detect Candida albicans Porphyromonas gingivalis . Our motivation lies in prevalence these microorganisms subgingival area where frequency fungal colonization increases with periodontal disease. This work is based on that both therapeutic induces a color change nanoparticle reporter. was then built into zwitterionic prodrug quenches its activity until activation by protease inherent pathogens interest: SAP9 or RgpB for C. P. , respectively. first confirmed intact has negligible toxicity fungal, bacterial, mammalian cells absent trigger. Next, impact assessed via disk diffusion viability assays showed minimum inhibitory concentration 3.1 – 16 µg/mL, which comparable alone (absent integration prodrug). Finally, design exploited colorimetric detection proteases. When were cleaved, plasmonic nanoparticles aggregated causing limit 10 nM gold 3 silver nanoparticles. approach value as convenient selective sensing protease-induced treatment mechanism bioinspired peptides. Abstract Figure

Language: Английский

Nanocounter on cell membrane: In-situ quantification of membrane-bound enzymes with high simplicity and sensitivity via the assembly of branched peptides DOI

Yiwei Han,

K. Wang,

Jianyang Lu

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 410, P. 135649 - 135649

Published: March 16, 2024

Language: Английский

Citations

0
Activatable prodrug for controlled release of an antimicrobial peptide via the proteases overexpressed inCandida albicansandPorphyromonas gingivalis DOI Open Access
Lubna Amer, Maurice Retout, Jesse V. Jokerst

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 27, 2023

ABSTRACT We report the controlled release of an antimicrobial peptide using enzyme-activatable prodrugs to treat and detect Candida albicans Porphyromonas gingivalis . Our motivation lies in prevalence these microorganisms subgingival area where frequency fungal colonization increases with periodontal disease. This work is based on that both therapeutic induces a color change nanoparticle reporter. was then built into zwitterionic prodrug quenches its activity until activation by protease inherent pathogens interest: SAP9 or RgpB for C. P. , respectively. first confirmed intact has negligible toxicity fungal, bacterial, mammalian cells absent trigger. Next, impact assessed via disk diffusion viability assays showed minimum inhibitory concentration 3.1 – 16 µg/mL, which comparable alone (absent integration prodrug). Finally, design exploited colorimetric detection proteases. When were cleaved, plasmonic nanoparticles aggregated causing limit 10 nM gold 3 silver nanoparticles. approach value as convenient selective sensing protease-induced treatment mechanism bioinspired peptides. Abstract Figure

Language: Английский

Citations

0