Microenvironment-adaptive nanomedicine MXene promotes flap survival by inhibiting ROS cascade and endothelial pyroptosis

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 7, 2025

In the field of large-area trauma flap transplantation, preventing avascular necrosis remains a critical challenge. Key mechanisms for improving viability include angiogenesis promotion, oxidative stress inhibition, and cell death prevention. Recently, two-dimensional ultrathin Ti3C2TX (MXene) nanosheets have gained attention their potential contributions to these processes, though MXene's physiological impact on survival had not been previously investigated. This study is first confirm biological effects ischaemic microenvironment post-skin transplantation. Findings indicated that MXene significantly decreased necrotic area in flaps (37.96% ± 2.00%), with reductions 30.40% 1.86% at 1 mg/mL 20.19% 2.11% 2 concentration-dependent manner. Mechanistically, facilitated situ angiogenesis, mitigated stress, suppressed pro-inflammatory pyroptosis, activated PI3K-Akt pathway, particularly influencing vascular endothelial cells. Comparative transcriptome analysis skin tissues without treatment provided additional evidence, highlighting such as ROS metabolic proliferation regulation, signaling pathway activation. Overall, demonstrated activity, effectively promoting presenting novel strategy addressing flaps.

Language: Английский

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Mussel-Inspired MXene/Antimicrobial Peptide-Integrated Photosensitive Poly(vinyl alcohol)-Based Hydrogel with Antibacterial, Anti-Inflammatory, and Electroactive Properties for Accelerated Wound Healing

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Backgrounds: The buildup of reactive oxygen species (ROS) in infected wounds triggers an excessive inflammatory response, while the overuse antibiotics has contributed to increased bacterial resistance. Therefore, developing wound dressings that effectively eliminate ROS and inhibit growth is crucial. Methods: Inspired by mussel-derived proteins, we developed a polydopamine (PDA)-grafted MXene (PDA@MXene) 3,4-dihydroxyphenylalanine-PonG1 (DOPA-PonG1)-modified photosensitive poly(vinyl alcohol) (PVA) hydrogel as dressing. PDA@MXene was synthesized through dopamine self-polymerization on surface, tyrosine hydroxylation used introduce DOPA into antibacterial peptide ponericin G1 (PonG1). its components were characterized, their morphology examined. hydrogel's hemostatic ability, mechanical properties, conductivity evaluated. In vitro studies systematically evaluated antioxidative effects, activity, biocompatibility, expression tissue regeneration-related factors. An full-thickness skin defect model established vivo, different treatments applied. wound-healing rate then measured, followed histological analysis using hematoxylin eosin, Masson, Sirius Red, immunofluorescence staining investigate healing mechanism. Results: sequence enhanced PonG1 stability leading sustained ability. significantly improved strength. Notably, combined effects DOPA-PonG1 ROS-scavenging properties. vivo findings demonstrated DOPA-PonG1/PDA@MXene/PVA accelerated promoting angiogenesis collagen deposition reducing inflammation. This study presents innovative approach for treating defects holds promise clinical applications.

Language: Английский

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Injectable dual-network hydrogel for phototherapy and immunomodulation in the treatment of MRSA-infected and diabetic wound

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163026 - 163026

Published: April 1, 2025

Language: Английский

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Pluripotent polysaccharide coordinated hydrogels remodel inflammation, neovascularization and reepithelization for efficient diabetic wound prohealing

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 377, P. 37 - 53

Published: Nov. 17, 2024

Language: Английский

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From conventional to cutting-edge: Exosomes revolutionizing nano-drug delivery systems

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 156685 - 156685

Published: Oct. 1, 2024

Language: Английский

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